Detection by PCR of wild-type canine parvovirus which contaminates dog vaccines

Senda, M.; Parrish, Colin R.; Harasawa, Ryô; Gamoh, Koichiro; Muramatsu, Masatake; Hirayama, Norio; Itoh, Osamu

doi:10.1128/jcm.33.1.110-113.1995

Cited by 65 publications

(39 citation statements)

References 13 publications

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“…A PCR-based approach has been proposed by Senda et al (1995) to address this point, which takes advantage of two SNPs, A3045T and C3685G, that determine the replacement of Met by Leu at position 87 and of Ala by Gly at position 300, in old-and wild-type strains, respectively (Table 1). Two primers specific for the wild types (types 2a and 2b) were selected to have one such mutation at the very 3 end, as nucleotide mismatches that occur at the 3 end of a primer are highly detrimental to primer extension and strongly decrease PCR amplification.…”

Section: Discussionmentioning

confidence: 99%

A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus

Decaro¹,

Elia²,

Desario³

et al. 2006

Journal of Virological Methods

104

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A minor groove binder (MGB) probe assay was developed to discriminate between type 2-based vaccines and field strains of canine parvovirus (CPV). Considering that most of the CPV vaccines contain the old type 2, no longer circulating in canine population, two MGB probes specific for CPV-2 and the antigenic variants (types 2a, 2b and 2c), respectively, were labeled with different fluorophores. The MGB probe assay was able to discriminate correctly between the old type and the variants, with a detection limit of 10(1) DNA copies and a good reproducibility. Quantitation of the viral DNA loads was accurate, as demonstrated by comparing the CPV DNA titres to those calculated by means of the TaqMan assay recognising all CPV types. This assay will ensure resolution of most diagnostic problems in dogs showing CPV disease shortly after CPV vaccination, although it does not discriminate between field strains and type 2b-based vaccines, recently licensed to market in some countries.

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Section: Discussionmentioning

confidence: 99%

A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus

Decaro¹,

Elia²,

Desario³

et al. 2006

Journal of Virological Methods

104

View full text Add to dashboard Cite

show abstract

“…To address this point, a PCR-based approach has been proposed by Senda et al (1995), taking advantage of two SNPs, A3045T and C3685G, that determine the replacement of methionine by leucine at position 87 and of alanine by glycine at position 300, in old and wild-type strains, respectively (Table 1). However, in our experience, such mutations were not sufficient to prevent completely amplification of the old-type virus (vaccine) (personal observation).…”

Section: Discrimination Between Vaccine and Field Strainsmentioning

confidence: 99%

Canine parvovirus—A review of epidemiological and diagnostic aspects, with emphasis on type 2c

Decaro

Buonavoglia

2012

Veterinary Microbiology

420

458

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Canine parvovirus type 2 (CPV-2) emerged in late 1970s causing severe epizootics in kennels and dog shelters worldwide. Soon after its emergence, CPV-2 underwent genetic evolution giving rise consecutively to two antigenic variants, CPV-2a and CPV-2b that replaced progressively the original type. In 2000, a new antigenic variant, CPV-2c, was detected in Italy and rapidly spread to several countries. In comparison to the original type CPV-2, the antigenic variants display increased pathogenicity in dogs and extended host range, being able to infect and cause disease in cats. Epidemiological survey indicate that the newest type CPV-2c is becoming prevalent in different geographic regions and is often associated to severe disease in adult dogs and also in dogs that have completed the vaccination protocols. However, the primary cause of failure of CPV vaccination is interference by maternally derived immunity. Diagnosis of CPV infection by traditional methods has been shown to be poorly sensitive, especially in the late stages of infections. New diagnostic approaches based on molecular methods have been developed for sensitive detection of CPV in clinical samples and rapid characterisation of the viral type. Continuous surveillance will help assess whether there is a real need to update currently available vaccines and diagnostic tests.

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“…Genomic DNA was extracted from the fecal samples by phenol and chloroform method. Specific primer pair as reported by Senda et al (1995) was used with slight modification to identify CPV2a/2b variants. These primers amplified a portion of VP1/VP2 gene of both CPV2a and CPV2b variants to yield a product size of 681 bp.…”

Section: Collection and Processing Of Fecal Samplesmentioning

confidence: 99%

Oxidative stress indices in gastroenteritis in dogs with canine parvoviral infection

Panda

Patra

Nandi

et al. 2009

Research in Veterinary Science

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Gastroenteritis of viral origin has emerged as a major cause of morbidity and mortality in dogs during the last two decades. Amongst the viral etiologies responsible for gastroenteritis in dogs, canine parvovirus (CPV) is considered as the most pathogenic. The disease is characterized by hemorrhagic enteritis, bloody diarrhoea and myocarditis in young pups. The present study was carried out to examine alterations in oxidative stress indices in the erythrocytes from dogs suffering from gastroenteritis with or without canine parvoviral infection as confirmed by CPV-DNA amplification from faeces using specific primers for CPV-2 as well as CPV-2a and CPV-2b variants by polymerase chain reaction (PCR). The present investigation utilized clinical cases of dogs with signs of acute diarrhea (n=56), and 14 more apparently healthy dogs of similar age group. Erythrocytic oxidative stress indices such as lipid peroxides level and antioxidant enzymes like superoxide dismutase and catalase activity, and blood micro-mineral (iron, copper, cobalt and zinc) status were analyzed in each dog (n=70). The acute cases of gastroenteritis in dogs were associated with altered erythrocytic lipid peroxidation as evident by estimation of malonaldehyde (MDA) concentration. The activities of antioxidant enzymes catalase and superoxide dismutase, the first line of antioxidant defense against damaging effects of free radicals, were also altered. The alterations in oxidative stress indices were more pronounced in cases with involvement of canine parvovirus as compared to parvo-negative cases. Our results also revealed decreased blood zinc level in diarrhoea in dogs irrespective of involvement of canine parvovirus.

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Detection by PCR of wild-type canine parvovirus which contaminates dog vaccines

Cited by 65 publications

References 13 publications

A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus

A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus

Canine parvovirus—A review of epidemiological and diagnostic aspects, with emphasis on type 2c

Oxidative stress indices in gastroenteritis in dogs with canine parvoviral infection

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