Detection by polmerase chain reaction-amplification and sequencing of an archaeon in a commercial-scale copper bioleaching plant

Vásquez, Mónica; Moore, Edward R. B.; Espejo, Romilio T.

doi:10.1111/j.1574-6968.1999.tb13500.x

Cited by 13 publications

(4 citation statements)

References 20 publications

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“…Archaeal organisms are also important members of microbial communities that are found in hot, extremely acidic, metal-rich environments (Vasquez et al 1999;Burton and Norris 2000). There are three divisions: Thermoplasma, Ferroplasma and Sulfolobales which have been detected to be prevalent in natural AMD environments (Bond et al 2000b;Baker and Banfield 2003;Druschel et al 2004a).…”

Section: Bacelarmentioning

confidence: 99%

Microbial populations in acid mineral bioleaching systems of Tong Shankou Copper Mine, China

Xie

Xiao

et al. 2007

Journal of Applied Microbiology

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Aims: To understand the composition and structure of microbial communities in different acid mineral bioleaching systems, and to present a more complete picture of microbially mediated acid mine drainage production. Methods and Results: In Tong Shankou Copper Mine, China, two samples (named K1 and K2) from two different sites with bioleaching were studied. A bacterial 16S rDNA library and an archaeal 16S rDNA library of the sample from each site were constructed by 16S rDNA polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and sequencing. A total of 18 bacterial representative sequences and 12 archaeal representative sequences were obtained. Phylogenetic analysis indicated that 77·09% of the total bacterial clones were affiliated with Proteobacteria, and 21·22% of the total bacterial clones were closely related to Nitrospira. The rest of the bacterial clones were related to Firmicutes (1·68%). Sequences affiliated with the archaea of the Thermoplasma and Ferroplasma lineages were detected abundantly in the two samples. Unexpectedly, sequences affiliated with Sulfolobales and Methanothermus genera were also detected. Conclusions: The molecular studies appear to be consistent with the environmental conditions existing at the sites, which coincides with previous studies. High concentrations of some elements (such as copper, iron and sulfur) seemed to be the key factors resulting in the diverse distribution of typical iron‐oxidizing bacteria such as Leptospirillum species and Acidithiobacillus ferrooxidans. Significance and Impact of the Study: Research on micro‐organisms present in bioleaching systems especially archaea is not abundant. The acidophiles in the two bioleaching sites obtained from Tong Shankou Copper Mine, China, have not been reported until now. These results may expand our knowledge of the microbial diversity in the acid mineral bioleaching systems.

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Section: Bacelarmentioning

confidence: 99%

Microbial populations in acid mineral bioleaching systems of Tong Shankou Copper Mine, China

Xie

Xiao

et al. 2007

Journal of Applied Microbiology

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“…One is the previously mentioned F. acidiphilum isolate, obtained from a semi-industrial bioleaching reactor in Eastern Europe [Kazakhstan (12)l. In the other study (Chile; no isolate was obtained), Thermoplasmales were the predominate phylotype in a bioleaching reactor when it was operated under high-sulfate (low-pH) conditions (16). The high metal and sulfate conditions under which some bioleaching reactors are operated may be more representative of highly concentrated subsurface AMD sites than the diluted run-off waters that are more commonly studied.…”

mentioning

confidence: 94%

An Archaeal Iron-Oxidizing Extreme Acidophile Important in Acid Mine Drainage

Edwards

Bond

Gihring

et al. 2000

Science

500

258

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A new species of Archaea grows at pH approximately 0.5 and approximately 40 degrees C in slime streamers and attached to pyrite surfaces at a sulfide ore body, Iron Mountain, California. This iron-oxidizing Archaeon is capable of growth at pH 0. This species represents a dominant prokaryote in the environment studied (slimes and sediments) and constituted up to 85% of the microbial community when solution concentrations were high (conductivity of 100 to 160 millisiemens per centimeter). The presence of this and other closely related Thermoplasmales suggests that these acidophiles are important contributors to acid mine drainage and may substantially impact iron and sulfur cycles.

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“…Rawlings, unpublished data). More recently, a related archaeon was found in copper ore column/heap-type plants operating at 30 • C at high salt concentration (95). Although different from each other, 16S rRNA sequence data indicated that the two archaea isolates were most closely related to Picrophilus oshimae and Thermoplasma acidophilum.…”

Section: Potentially Important Bacteria In Mineral Biooxidation Procementioning

confidence: 98%

Heavy Metal Mining Using Microbes

Rawlings

2002

Annu. Rev. Microbiol.

568

310

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The use of acidiphilic, chemolithotrophic iron- and sulfur-oxidizing microbes in processes to recover metals from certain types of copper, uranium, and gold-bearing minerals or mineral concentrates is now well established. During these processes insoluble metal sulfides are oxidized to soluble metal sulfates. Mineral decomposition is believed to be mostly due to chemical attack by ferric iron, with the main role of the microorganisms being to reoxidize the resultant ferrous iron back to ferric iron. Currently operating industrial biomining processes have used bacteria that grow optimally from ambient to 50 degrees C, but thermophilic microbes have been isolated that have the potential to enable mineral biooxidation to be carried out at temperatures of 80 degrees C or higher. The development of higher-temperature processes will extend the variety of minerals that can be commercially processed.

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Detection by polmerase chain reaction-amplification and sequencing of an archaeon in a commercial-scale copper bioleaching plant

Cited by 13 publications

References 20 publications

Microbial populations in acid mineral bioleaching systems of Tong Shankou Copper Mine, China

Microbial populations in acid mineral bioleaching systems of Tong Shankou Copper Mine, China

An Archaeal Iron-Oxidizing Extreme Acidophile Important in Acid Mine Drainage

Heavy Metal Mining Using Microbes

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