Detection, Identification, and Inactivation of Histamine-forming Bacteria in Seafood: A Mini-review

Au@Ag Core–Shell Nanoparticles for Colorimetric and Surface-Enhanced Raman-Scattering-Based Multiplex Competitive Lateral Flow Immunoassay for the Simultaneous Detection of Histamine and Parvalbumin in Fish

“…The supernatant was filtered with a 0.22 μm filter and diluted 10 times with 1× PBS at pH = 7.4. Later, different concentrations of histamine (0.04, 0.4, 2, 4, 10, 20, 30, 40, 100, 300, and 4000 mg/kg) and/or parvalbumin (4,10,20,30,40,100,200,300,400,2000, and 4000 mg/kg) were spiked in the sample. Then, in a 96-well assay plate were mixed 20 μL of the sample, 4 μL of each SERS tag, and 80 μL of running buffer (1% casein (w/w), 3% Tween 20 (w/w) in phosphate buffer 10 mM and pH 7.4), and a strip is introduced in the mixture.…”

“…However, its application demands highly trained personnel on bulky and costly instrumentation typically found in centralized laboratories, making it unsuitable for on-site testing. Numerous studies have focused on detecting histamine and parvalbumin in fish, with each study examining these molecules individually − However, simultaneous detection of both parvalbumin , and histamine , can offer a more comprehensive assessment of the safety and freshness of fish for human consumption , …”

“…However, its application demands highly trained personnel on bulky and costly instrumentation typically found in centralized laboratories, making it unsuitable for on-site testing. Numerous studies have focused on detecting histamine and parvalbumin in fish, with each study examining these molecules individually 14−17 However, simultaneous detection of both parvalbumin 18,19 and histamine 20,21 can offer a more comprehensive assessment of the safety and freshness of fish for human consumption 22,23 Colorimetric lateral flow immunoassays (LFIAs) are analytical devices widely used for on-site diagnostics and environmental monitoring 24,25 that fulfill the WHO's AS-SURED criteria (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free, and Deliverable to end users). 26 Thus, LFIAs have been widely used in pregnancy tests, infectious disease detection, and drug and food safety testing, as well as for environmental monitoring.…”

Structural Model Comparison of Histidine Decarboxylase (Hdc) of Histamine-Producing Bacteria Morganella Morganii Constructed by Alphafold2 and Swissmodel

“…Morganella morganii which can grow on fishery products containing the amino acid histidine in high concentrations is reported to be responsible for producing the biogenic amine histamine. The histamine content in fishery products is a significant problem in fishery products, especially in the Scombridae family, because the accumulation of histamine can pose serious health risks for consumers (Nevado et al, 2023). Previous research has shown the presence of histamine in various fishery products, such as lemuru fish (Azzamudin et al, 2024), mackarel tuna (Pertiwi et al, 2020), and tuna (Witria et al, 2020).…”

“…HDC is one part of the decarboxylase in group II pyridoxal 5'-phosphate (PLP), catalyzing the synthesis of histamine from L-histidine with high substrate specificity (Takeshima et al, 2020). Various efforts have been made to inhibit the growth of HDC-producing bacteria, including temperature control through cold chains and high pressure processing (HPP) (Lee et al, 2020;Nevado et al, 2023). However, specific enzyme inhibition needs to be carried out with precise targeting based on the structure of the Morganella morganii HDC protein.…”