Detection, Identification and Molecular Characterization of a Phytoplasma Associated with Arabian Jasmine (<i>Jasminum sambac</i> L.) Witches’ Broom in Oman

Al-Zadjali, A. D.; Natsuaki, Tomohide; Okuda, Shigenobu

doi:10.1111/j.1439-0434.2007.01219.x

Cited by 22 publications

(15 citation statements)

References 30 publications

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“…In China and Vietnam it is used to produce the flowers used in jasmine tea and is grown in plantations. There are relatively few reports of disease of jasmine; these include witches' broom caused by phytoplasma (Al-Zadjali et al 2007), tomato mosaic virus infection (Kamenova et al 2006), cercosporoid leaf spots (Braun and Sivapalan 1999) and several other folicolous taxa (Agarwal and Sahni 1965;Sahni 1966). Reports of Colletotrichum species on jasmine are rare.…”

Section: Introductionmentioning

confidence: 96%

Colletotrichum species from Jasmine (Jasminum sambac)

et al. 2010

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Colletotrichum species associated with leaf and flower anthracnose of jasmine (Jasminum sambac) in the Ho Chi Minh region of Vietnam are reported. The disease of jasmine plantations was considered serious as it likely reduced flower yield. Leaves were colonized by Colletotrichum species which formed chlorotic regions with light brown necrotic centres, which eventually covered the whole leaf and subsequently caused defoliation and dieback and whole flowers were blighted. Nine strains of Colletotrichum species were isolated from diseased leaves and flowers and partial ITS rDNA sequences were analysed and morphologies compared across similar species. Based on ITS sequence analysis and morphological characters, three strains were identified as C. truncatum, while one strain was identified as C. siamense. The remaining five strains did not cluster with any known species for which type sequences are available and therefore partial actin (ACT), β-tubulin (TUB2), calmodulin (CAL), glutamine synthetase (GS), glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes of the isolates were sequenced. Based on the reconstructed multiloci molecular phylogeny, two taxa are formally introduced as new species. Another strain was not well resolved in the phylogenetic tree and herein described as Colletotrichum sp. Further studies are needed to prove its distinctiveness. The morphology and growth rate of all taxa are described and compared with similar species.

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Section: Introductionmentioning

confidence: 96%

Colletotrichum species from Jasmine (Jasminum sambac)

et al. 2010

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“…Phytoplasma asteris' in Lithuania (Jomantiene et al 2000) caused alfalfa witches'-broom disease. In Asia, phytoplasmas of the 16SrII-D subgroup in Oman (Khan et al 2002, Alzadjali et al 2007, the elm yellows group in China (Li et al 2012) and a mycoplasma-like organism in Saudi Arabia (Cook & Wilton 1985) were identified as causal agents of alfalfa witches'-broom. Moreover, 'Ca.…”

Section: Introductionmentioning

confidence: 99%

Characterization of phytoplasmas related to peanut witches’-broom and stolbur groups associated with alfalfa diseases in Iran

2013

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In the central and south eastern provinces of Iran, alfalfa plants showing phytoplasma diseases-like symptoms categorized into three major types, namely witches'-brooms, little leaf and yellowing, were observed during 2008 and 2009. Phytoplasmas were detected by PCR assays using phytoplasma universal primer pair P1/P7 followed by nested PCR using primer pair R16F2n/R16R2. PCR results confirmed the phytoplasma infection in symptomatic alfalfa plants. Restriction fragment length polymorphism (RFLP) and sequence analysis of partial 16S rRNA gene from the Iranian alfalfa phytoplasmas indicated that phytoplasmas of the 16SrII and 16SrXII groups were associated with alfalfa witches'-broom and alfalfa yellows, respectively, while phytoplasmas of the 16SrII-D subgroup are associated with alfalfa little leaf disease. This research is the first study to report stolbur phytoplasmas as causal agents of alfalfa yellows and 16SrII-D phytoplasmas as causal agents of alfalfa little leaf diseases in Iran.

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“…2005). Phytoplasmas appear to be quasi‐isometric with a mean diameter of 200–300 nm (Al‐Zadjali et al. 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Plants infected with phytoplasmas exhibit a range of symptoms such as virescence, phyllody, yellows, witches’ broom, leaf rolling and general decline (Bertaccini et al. 2005) and also stunting, floral abnormalities, shoot proliferation, yellowing of foliage and reduced vigour, often leading to the death of the plant (Al‐Zadjali et al. 2007).…”

Section: Introductionmentioning

confidence: 99%

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Identification of New Members of Candidatus Phytoplasma asteris Affecting Tomato Plants in Poland

Krawczyk¹,

Pospieszny²,

Kamasa³

2010

Journal of Phytopathology

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Two symptomatic tomato plants exhibiting dwarfing, twisting of shoots and leaves, virescence and phyllody of flowers were collected, respectively, from a greenhouse (Soly07fi) or the field (Soly06gh) in the western region of Poland. Direct and nested polymerase chain reactions (PCR) were performed using universal phytoplasma primers P1 ⁄ P7 and R16F2n ⁄ R16R2. Restriction fragment length polymorphism (RFLP) analysis of the PCR products showed that the RFLP profiles of both tested phytoplasma isolates are the same and that they belong to the phytoplasma 16S rRNA I-C subgroup. The homology between the two strains was 99%. Phylogenetic analysis of the 16S rRNA gene sequences of the phytoplasma isolates and other phytoplasma sequences available in the GenBank database indicated that the Polish phytoplasma isolates are most closely related to the phytoplasma 16S rRNA I-C subgroup.

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Detection, Identification and Molecular Characterization of a Phytoplasma Associated with Arabian Jasmine (Jasminum sambac L.) Witches’ Broom in Oman

Cited by 22 publications

References 30 publications

Colletotrichum species from Jasmine (Jasminum sambac)

Colletotrichum species from Jasmine (Jasminum sambac)

Characterization of phytoplasmas related to peanut witches’-broom and stolbur groups associated with alfalfa diseases in Iran

Identification of New Members of Candidatus Phytoplasma asteris Affecting Tomato Plants in Poland

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