Identification of New Members of <i>Candidatus</i> Phytoplasma asteris Affecting Tomato Plants in Poland

Krawczyk, Krzysztof; Pospieszny, H.; Kamasa, Joanna

doi:10.1111/j.1439-0434.2009.01648.x

Cited by 7 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phytoplasma diseases of tomato are attributed to different bacterial hosts worldwide, including "Ca. P. asteris" (Krawczyk et al, 2010), "Ca. P. aurantifolia" (Dong et al, 2013), "Ca.…”

Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch's broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the "Canditatus Phytoplasma solani" (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the "Canditatus Phytoplasma trifolii" (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as 'Bingöl D11' and 'Bingöl D90' isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong. This current paper is documented in the first record of "Ca. P. solani" (16SrXII-A) and "Ca. P. trifolii" (16SrVI-A) in naturally diseased tomato in Bingöl of Turkey.

show abstract

“…Phytoplasma diseases of tomato are attributed to different bacterial hosts worldwide, including "Ca. P. asteris" (Krawczyk et al, 2010), "Ca. P. aurantifolia" (Dong et al, 2013), "Ca.…”

Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

show abstract

“…Total nucleic acid of leaf midribs and petioles was extracted using the cetyl trimethyl ammonium bromide (CTAB) extraction protocol (Krawczyk et al. ). For PCR, universal phytoplasma R16F2n/R16R2 primers were used to amplify a portion of the 16S rDNA (Gundersen and Lee ).…”

Section: Methodsmentioning

confidence: 99%

Detection and Characterization of Phytoplasma Associated with Big Bud Disease of Tomato in China

Mou

Zhu

et al. 2012

Journal of Phytopathology

View full text Add to dashboard Cite

Symptomatic tomato plants exhibiting big bud, proliferation and small leaves of lateral shoots, purplish top leaves, phyllody, enlarged pistils, hypertrophic calyxes and small and polygonal fruit were collected in Yunnan Province of China. Pleomorphic phytoplasma-like bodies were observed in the phloem sieve tube elements of symptomatic plants by transmission electron microscopy. The presence of phytoplasma in collected samples was further analysed and identified by PCR and virtual computer-simulated restriction fragment length polymorphism (virtual RFLP). A 1.2 kb product was amplified by PCR with universal primers R16F2n/R16R2. Sequence comparisons revealed that the tested strains shared 99% 16S rRNA gene sequence similarity with members of 'Candidatus Phytoplasma aurantifolia' (16SrII group). Phylogenetic and virtual RFLP analysis of the 16S rRNA gene sequences confirmed that the phytoplasma is a member of the 16SrII group. This is the first report of 16SrII group phytoplasma infecting tomato in China.*These two authors contributed equally to this study.

show abstract

“…The principal phytoplasma groups infecting tomato are in group 16SrI, 16SrII, 16SrIII, 16SrV, and 16SrVI (Zamora et al, 2014;Singh et al, 2012;Amaral-Mello et al, 2006;Del Serrone et al, 2001;Anfoka et al, 2003), which induce mostly similar symptoms on their respective host (Krawczyk et al, 2010;Pracros et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey

Usta

Güller

2020

Turkish JAF Sci.Tech.

View full text Add to dashboard Cite

The tomato plant exhibiting leaf rolling, witches’ broom, distorted and elongated ﬂower’s sepals in Iğdır province, Turkey, was observed. Total DNA extraction was performed from the symtomatic fresh tomato sample. All DNAs were subjected to Direct and Nested polymerase chain reaction (PCR) with universal primer sets that amplified the 16S rRNA of phytoplasmas. PCR products were purified from agarose gel and cloned into the pGEM T-Easy cloning vector. Recombinant plasmids were introduced into the prokaryotic cloning bacteria by electroporation. Plasmid isolation was performed by selecting one of the positive clones randomly and sequencing was performed by Next Generation Sequencing (NGS). Sequencing results revealed that the 16S rRNA gene associated with phytoplasma was 1251 nucleotides in size, and this sequence was denominated as ‘Iğdır 10’ isolates and recorded in the GenBank under the MT344968 accession number. The virtual restriction fragment length polymorphism (V-RFLP) and phylogenetic analysis of the 16S rDNA sequence confirmed that the cause of disease in infected tomato plants was 'Candidatus Phytoplasma trifolii' ('Ca. P. trifolii') (16SrVI-A, Clover proliferation group), with a 1.00 similarity coefficient. This present study is the first report of ‘Ca. P. trifolii’ and its nucleotide sequence analysis in naturally infected tomato in Iğdır province.

show abstract

Identification of New Members of Candidatus Phytoplasma asteris Affecting Tomato Plants in Poland

Cited by 7 publications

References 22 publications

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Detection and Characterization of Phytoplasma Associated with Big Bud Disease of Tomato in China

Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey

Contact Info

Product

Resources

About