Detection Methods Using Chemiluminescence

Bronstein, Irena; Olesen, Corinne E.M.

doi:10.1016/b978-012748920-9/50008-x

Cited by 7 publications

(4 citation statements)

References 99 publications

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“…Thus, ECL has been used for various applications, such as clinical diagnosis, environmental monitoring, and food monitoring [94][95][96]. However, ECL has the disadvantage of requiring instruments, including emission detection systems [97,98]. Liu et al used AgNPs to form an immunoaffinity layer to detect cyclin D1 [99].…”

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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Biosensors used for medical diagnosis work by analyzing physiological fluids. Antibodies have been frequently used as molecular recognition molecules for the specific binding of target analytes from complex biological solutions. Electrochemistry has been introduced for the measurement of quantitative signals from transducer-bound analytes for many reasons, including good sensitivity. Recently, numerous electrochemical immunosensors have been developed and various strategies have been proposed to detect biomarkers. In this paper, the recent progress in electrochemical immunosensors is reviewed. In particular, we focused on the immobilization methods using antibodies for voltammetric, amperometric, impedimetric, and electrochemiluminescent immunosensors.

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Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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“…After hybridization, mRNA, small RNA, or miRNA expression can be detected using the biotin-streptavidin-HRP conjugation method. X-ray has been traditionally used for the detection of HRP-luminol [52]. HRP oxidizes its chemiluminescent substrate luminol in the presence of peroxide and emits light at 425 nm that can be detected using an X-ray film [52].…”

Section: Detection Of False-positive Signals After Using Machine-based Detectionmentioning

confidence: 99%

“…X-ray has been traditionally used for the detection of HRP-luminol [52]. HRP oxidizes its chemiluminescent substrate luminol in the presence of peroxide and emits light at 425 nm that can be detected using an X-ray film [52]. Nowadays, machine-based laser scanners are replacing the traditional X-ray technique and are being widely used for the detection of nucleic acids and proteins.…”

Section: Detection Of False-positive Signals After Using Machine-based Detectionmentioning

confidence: 99%

A Comprehensive Analysis of Northern versus Liquid Hybridization Assays for mRNAs, Small RNAs, and miRNAs Using a Non-Radiolabeled Approach

Ahmad

Gull

Baby

et al. 2021

CIMB

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Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity. With the emergence of the field of microRNAs (miRNAs), the necessity for sensitive and quantitative NBs has again emerged. Here, we developed highly sensitive yet non-radiolabeled, fast, economical NB, and liquid hybridization (LH) assays without radioactivity or specialized reagents like locked nucleic acid (LNA)- or digoxigenin-labeled probes for mRNAs/small RNAs, especially miRNAs using biotinylated probes. An improvised means of hybridizing oligo probes along with efficient transfer, cross-linking, and signal enhancement techniques was employed. Important caveats of each assay were elaborated upon, especially issues related to probe biotinylation, use of exonuclease, and bioimagers not reported earlier. We demonstrate that, while the NBs were sensitive for mRNAs and small RNAs, our LH protocol could efficiently detect these and miRNAs using less than 10–100 times the total amount of RNA, a sensitivity comparable to radiolabeled probes. Compared to NBs, LH was a faster, more sensitive, and specific approach for mRNA/small RNA/miRNA detection. A comparison of present work with six seminal studies is presented along with detailed protocols for easy reproducibility. Overall, our study provides effective platforms to study large and small RNAs in a sensitive, efficient, and cost-effective manner.

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“…These assays have been shown to be more sensitive and specific than ELISA, with fewer false positives and negatives. However, they may be more expensive and require specialized equipment ( Bronstein and Olesen 1995 ). Western blot is a confirmatory test used to verify the presence of anti-HIV antibodies in samples that have tested positive by ELISA or chemiluminescence.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Efficacy of Photostimulated Chemiluminescence Assay for Detecting Anti-HIV Antibodies: A Retrospective Study

Han,

Wang,

Wang

et al. 2023

Polish Journal of Microbiology

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The transmission of human immunodeficiency virus (HIV) through blood poses a slightly increased risk. As a result, patients requiring blood transfusions should be screened for HIV antibodies. This study examined the diagnostic effectiveness of the photostimulated chemiluminescence assay in detecting anti-HIV antibodies and determined the cut-off value for this method. The performance of the fully automated photostimulated chemiluminescence assay system was validated according to CNAS-GL038:2019 (2020) and CNAS-GL037:2019 (2019) guidelines. A retrospective study was conducted at the Department of Medical Laboratory, Nanjing Tongren Hospital, affiliated with Southeast University, from January 2020 to December 2022. A total of 77,386 cases were tested for anti-HIV antibodies using the photostimulated chemiluminescence assay, with 79 cases initially testing positive. The method’s performance in detecting anti-HIV antibodies was evaluated using the Receiver Operating Characteristic (ROC) curve and the average Coefficient of Variation (CV) value of 3-year in-house quality control. The precision, detection limit, coincidence rate, and critical value of the performance verification results met the requirements. Using Western blotting (WB) as the reference method, positive cases were initially screened using the light-induced chemiluminescence method to determine the cut-off index (COI) value and draw the ROC curve. The maximum area under the ROC curve using the chemiluminescence method was 0.997, with a cutoff value of < 28.56, sensitivity of 98%, specificity of 100%, Jordan index of 0.98, and an average CV value of 3.55%. In conclusion, the photostimulated chemiluminescence assay has good diagnostic efficacy in detecting anti-HIV antibodies and is suitable for rapid screening before blood transfusion and surgery.

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Detection Methods Using Chemiluminescence

Cited by 7 publications

References 99 publications

Recent Progress in Electrochemical Immunosensors

Recent Progress in Electrochemical Immunosensors

A Comprehensive Analysis of Northern versus Liquid Hybridization Assays for mRNAs, Small RNAs, and miRNAs Using a Non-Radiolabeled Approach

Diagnostic Efficacy of Photostimulated Chemiluminescence Assay for Detecting Anti-HIV Antibodies: A Retrospective Study

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