Recent Progress in Electrochemical Immunosensors

Kim, JeeYoung; Park, Min

doi:10.3390/bios11100360

Cited by 53 publications

(38 citation statements)

References 111 publications

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“…It records the variation of EIS with time for different volume percentages of MPA, and its semicircle diameter indicates the magnitude of the impedance value. The impedance value of the MPA/ME hybrid self-assembled membrane gradually increased with the extension of the assembly time and reached saturation adsorption at 24 h. As the volume percentage of MPA decreases, the impedance value becomes smaller when the MPA/ME hybrid self-assembled membrane reaches saturation adsorption [ 27 ], and the results are consistent with the CV characterization results.…”

Section: Resultssupporting

confidence: 70%

Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

et al. 2022

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A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrodes were placed in a 3-mercaptopropionic acid (MPA)/β-mercaptoethanol (ME) mixture for 24 h for self-assembly. The electrodes were then placed in an EDC/NHS mixture for 1 h. Layer modification was performed by stepwise dropwise addition of CA19-9 antibody, BSA, and antigen. Differential pulse voltammetry was used to characterize this immunosensor preparation process. The assembled electrochemical immunosensor enables linear detection in the concentration range of 0.05–500 U/mL of CA19-9, and the detection limit was calculated as 0.01 U/mL. The results of the specificity measurement test showed that the signal change of the interfering substance was much lower than the response value of the detected antigen, indicating that the sensor has good specificity and strong anti-interference ability. The repeatability test results showed that the relative standard deviations were less than 5%, showing good accuracy and precision. The CA19-9 electrochemical immunosensor was used for the actual sample detection, and the experimental results of the standard serum addition method showed that the RSD values of the test concentrations were all less than 10%. The recoveries were 102.4–115.0%, indicating that the assay has high precision, good accuracy, and high potential application value.

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Section: Resultssupporting

confidence: 70%

Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

et al. 2022

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“…The sample enrichment, very frequently observed in the case of solid-phase immobilization, is limited to the sensors based on surface modification [174], making the combination of capture and derivatization common for both approaches [175]. The magnetic nanoparticles, due to the additional separation feature, could be used in the preparation of electrodes for complex matrices, such as whole blood [176], since immunosensors with multilayer electrodes are gaining interest resulting in several papers with a title "Recent Advances in Electrochemical Immunosensors" [177][178][179].…”

Section: Discussion: Peptide Biosensors-advantages and Applicationsmentioning

confidence: 99%

Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

et al. 2022

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Analysis of peptide biomarkers of pathological states of the organism is often a serious challenge, due to a very complex composition of the cell and insufficient sensitivity of the current analytical methods (including mass spectrometry). One of the possible ways to overcome this problem is sample enrichment by capturing the selected components using a specific solid support. Another option is increasing the detectability of the desired compound by its selective tagging. Appropriately modified and immobilized peptides can be used for these purposes. In addition, they find application in studying the specificity and activity of proteolytic enzymes. Immobilized heterocyclic peptide conjugates may serve as metal ligands, to form complexes used as catalysts or analytical markers. In this review, we describe various applications of immobilized peptides, including selective capturing of cysteine-containing peptides, tagging of the carbonyl compounds to increase the sensitivity of their detection, enrichment of biological samples in deoxyfructosylated peptides, and fishing out of tyrosine–containing peptides by the formation of azo bond. Moreover, the use of the one-bead-one-compound peptide library for the analysis of substrate specificity and activity of caspases is described. Furthermore, the evolution of immobilization from the solid support used in peptide synthesis to nanocarriers is presented. Taken together, the examples presented here demonstrate immobilized peptides as a multifunctional tool, which can be successfully used to solve multiple analytical problems.

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“…As we already briefly mentioned in the Introduction section, biosensors are mostly classified in accordance with the biorecognition element and transduction principle. Thus, enzyme [ 123 , 124 ], immuno- [ 125 , 126 ], and DNA sensors [ 127 , 128 ] are used with appropriate biorecognition elements attached to the transducer interface or placed near them. Aptasensors containing aptamers specific to a certain analyte belong to the DNA sensors family [ 129 ].…”

Section: Principles Of Biosensors and Electrochemical Methods Of Anti...mentioning

confidence: 99%

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Evtugyn

Porfireva

Tsekenis

et al. 2022

Sensors

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Antibiotics are often used in human and veterinary medicine for the treatment of bacterial diseases. However, extensive use of antibiotics in agriculture can result in the contamination of common food staples such as milk. Consumption of contaminated products can cause serious illness and a rise in antibiotic resistance. Conventional methods of antibiotics detection such are microbiological assays chromatographic and mass spectroscopy methods are sensitive; however, they require qualified personnel, expensive instruments, and sample pretreatment. Biosensor technology can overcome these drawbacks. This review is focused on the recent achievements in the electrochemical biosensors based on nucleic acid aptamers for antibiotic detection. A brief explanation of conventional methods of antibiotic detection is also provided. The methods of the aptamer selection are explained, together with the approach used for the improvement of aptamer affinity by post-SELEX modification and computer modeling. The substantial focus of this review is on the explanation of the principles of the electrochemical detection of antibiotics by aptasensors and on recent achievements in the development of electrochemical aptasensors. The current trends and problems in practical applications of aptasensors are also discussed.

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Recent Progress in Electrochemical Immunosensors

Cited by 53 publications

References 111 publications

Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

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