Detection of 14 HLA-DQB1 alleles by oligotyping

Molkentin, Jeffery D.; Gorski, Jack; Baxter‐Lowe, Lee Ann

doi:10.1016/0198-8859(91)90014-z

Cited by 42 publications

(16 citation statements)

References 32 publications

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“…HLA-DQB1 was amplified using MCYFTNG.14 as the 5' primer (Molkentin et al, 1991) and as the 3' primer, 5'-GGTAGTTGTGTCTGCA-CAC-3' (data not shown). These primers generate a 210-bp fragment.…”

Section: Hla-dqbi Typingmentioning

confidence: 99%

Association between HLA‐DQB1 alleles and risk for cervical cancer in African‐American women

Grégoire

Lawrence

Kukuruga

et al. 1994

Intl Journal of Cancer

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Squamous-cell carcinoma of the cervix and its precursor lesions are associated with human papillomavirus (HPV) infection. Epidemiological studies indicate that HPV infection in itself is not sufficient for cervical-cancer induction, suggesting that other factors contribute to carcinogenesis. We have investigated the potential role of host genetic background as one such factor. We screened a series of squamous-cell carcinomas of the cervix for HLA-class-II DQB1* alleles by the polymerase chain reaction and site-specific oligonucleotide probe hybridization and for HPV type from African-American women using a local, ethnically matched control panel. Statistically significant associations for increase in relative risk for cervical cancer were seen for DQB1*0303 and DQB1*0604. DQB1*0201 and the heterozygote DQB1*0301/*0501 showed a decrease in relative risk for cervical cancer. HPV typing revealed no association between virus type and DQB1 alleles. Our results confirm other studies showing an increase in relative risk for cervical cancer associated with HLA-DQ3 alleles in Caucasians.

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Section: Hla-dqbi Typingmentioning

confidence: 99%

Association between HLA‐DQB1 alleles and risk for cervical cancer in African‐American women

Grégoire

Lawrence

Kukuruga

et al. 1994

Intl Journal of Cancer

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“…The preliminary application of SSOP/PCR methods to HLA typing of class I1 alleles has been extensively reported (8,21,31,32,33,34,35,36). We approached the task of desi,gning a typing system for DRw52-associated DRBl alleles with three goals in mind.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of HLA‐DRw52‐associated DRB1 genes as defined by sequence‐specific oligonucleotide probe hybridization and sequencing

et al. 1991

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We have used group-specific DNA amplification and sequence-specific oligonucleotide probe (SSOP) hybridization to study DRB1 sequence polymorphisms associated with DR3, DRw11(5), DRw12(5), DRw13(w6), DRw14(w6) and DRw8 alleles. Group-specific amplification of DRw52-associated DRB1 alleles was achieved using a 5' amplification primer designed to hybridize with a first hypervariable region (HVR) sequence common to all known alleles in this group, together with a 3' intron primer. Prospective SSOP typing of DR3, DRw11, DRw12, DRw13, DRw14 and DRw8 alleles was performed in 318 individuals, including 124 patients, 46 family members and 148 unrelated marrow donors. Among the 395 DRw52-associated DRB1 alleles tested in our study, a subtype corresponding to the previously defined alleles DRB1*0301-2 (DR3), DRB1*1101-4 (DR5), DRB1*1201-2 (DR5), DRB1*1301-5 (DRw6), DRB1*1401-2 and 1404 (DRw6), and DRB1*0801-4 (DRw8) could be assigned in all but 6 individuals (1.9%) tested. In addition to the 22 known alleles, we identified two new DRw6-associated alleles, DRB1*13.MW(1) and DRB1*14.GB(1). DRB1*13.MW typed serologically as DRw13 and was identical to DRB1*1301 except at codon 71 where AGG encodes arginine instead of GAG encoding glutamic acid. DRB1*14.GB represents a DRB1*1402 variant whose sequence at codon 86 encodes valine (GTG) instead of glycine (GGT). These results demonstrate that SSOP methods represent an efficient and precise approach for typing DRB1 alleles and for identifying potential novel variants previously unrecognized by conventional typing methods.

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“…commun.] or may be combined with dot-blot PCR-SSO typing [20,[43][44][45][46][47][48][49][50][51], reverse dot-blot PCR-SSO typing [25] or PCR-RFLP [29,33,34,36,381.…”

Section: Locus- Group-and Allele-specific Amplificationmentioning

confidence: 99%

Applications of the Polymerase Chain Reaction to HLA Class II Typing

Bidwell¹

1992

Vox Sanguinis

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Detection of 14 HLA-DQB1 alleles by oligotyping

Cited by 42 publications

References 32 publications

Association between HLA‐DQB1 alleles and risk for cervical cancer in African‐American women

Association between HLA‐DQB1 alleles and risk for cervical cancer in African‐American women

Polymorphism of HLA‐DRw52‐associated DRB1 genes as defined by sequence‐specific oligonucleotide probe hybridization and sequencing

Applications of the Polymerase Chain Reaction to HLA Class II Typing

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