Detection of a mixed-backbone oligonucleotide (GEM 231) in liver and tumor tissues by capillary electrophoresis

Bansal, Reeta; Chen, Helen X; Marshall, John L.; Tan, Jie; Glazer, Robert I.; Wainer, Irving W.

doi:10.1016/s0378-4347(00)00436-9

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Polyacrylamide gel electrophoresis and high-performance liquid chromatography (reversed-phase and anionexchange mode) have long been applied for various antisense analyses [5][6][7][8]. Capillary zone electrophoresis of antisense was recently demonstrated [9,10]. Also, electrospray ionization mass spectrometry (ESI-MS) [11], capillary gel electrophoresis on-line to ESI-MS [12] and matrix-assisted laser desorption/ionization mass spectrometry [13,14] are frequently used for such analyses and adds other analytical information such as, e.g.…”

Section: Introductionmentioning

confidence: 99%

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On-column electroextraction and separation of antisense oligonucleotides in human plasma by capillary gel electrophoresis

Palm

Markó-Varga

2004

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

“…Extraction of antisense from tissue, plasma, or serum is mostly devoted to liquid-liquid extractions, e.g. by phenol/chloroform, and/or solid-phase extraction techniques like ion-exchange and reversed-phase [8,9,11,13,[15][16][17][18][19][20][21]. These techniques often affords adequate yield and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

On-column electroextraction and separation of antisense oligonucleotides in human plasma by capillary gel electrophoresis

Palm

Markó-Varga

2004

Journal of Pharmaceutical and Biomedical Analysis

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“…Sound quality control is of major importance to give the ensuing analytical procedure, using these oligonucleotides, the needed degree of certainty. The therapeutic use of antisense oligonucleotides requires not only a high degree of quality control but also identification of minor impurities, as well as monitoring of metabolic products generated in vivo [88][89][90][91][92][93][94][95].…”

Section: Oligonucleotidesmentioning

confidence: 99%

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

et al. 2005

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This review is focused on the capillary electrophoresis-mass spectrometric (CE-MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE-MS interfaces and the difficulties associated with quantitative CE-MS are briefly discussed. A large section is finally devoted to field-oriented applications.

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Capillary electrophoresis with on-line enrichment for the analysis of biological samples

Sentellas

Puignou

Galcerán³

2002

J. Sep. Science

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“On‐Line” preconcentration in capillary electrophoresis (CE) is a good strategy to overcome the low concentration sensitivity of the technique, which is the main limitation when trace‐level compounds are to be determined in complex matrices such as biological samples. Here we discuss the use of at‐, on‐, and in‐line preconcentration procedures in combination with CE in the analysis of biological fluids. The preconcentration strategies discussed are classified into two groups depending on the mechanism involved: chromatographic or electrophoretic techniques. Recent applications are included and the advantages and drawbacks of the techniques are examined.

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Detection of a mixed-backbone oligonucleotide (GEM 231) in liver and tumor tissues by capillary electrophoresis

Cited by 4 publications

References 20 publications

On-column electroextraction and separation of antisense oligonucleotides in human plasma by capillary gel electrophoresis

On-column electroextraction and separation of antisense oligonucleotides in human plasma by capillary gel electrophoresis

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

Capillary electrophoresis with on-line enrichment for the analysis of biological samples

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