Detection of a novel hemoplasma based on 16S rRNA gene DNA in captive and free-ranging capybaras (Hydrochaeris hydrochaeris)

Vieira, Rafael Felipe da Costa; Molento, Marcelo Beltrão; Santos, Leonilda Correia dos; Moraes, Wanderlei de; Cubas, Zalmir Silvino; Santos, Andrea Pires dos; Guimarães, Ana M. S.; Mohamed, Ahmed; Filho, Ivan Roque de Barros; Biondo, Alexander Welker; Messick, Joanne B.

doi:10.1016/j.vetmic.2009.06.018

Cited by 31 publications

(29 citation statements)

References 12 publications

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“…(hemoplasmas, formerly classified as Haemobartonella and Eperythrozoon spp.) (4,12,28,29) appear to have coevolved with animals, including dogs, cats, humans, alpacas, capybaras, and sea lions (1,10,13,(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40). The development of molecular assays, which target primarily the 16S rRNA gene of these microbes, has resulted in the more recent recognition of several novel animal hemoplasmas (5,12,37,41).…”

Section: Discussionmentioning

confidence: 99%

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“Candidatus Mycoplasma haemomacaque” and Bartonella quintana Bacteremia in Cynomolgus Monkeys

et al. 2013

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Here, we report latent infections with Bartonella quintana and a hemotropic Mycoplasma sp. in a research colony of cynomolgus monkeys (Macaca fascicularis). Sequence alignments, evolutionary analysis, and signature nucleotide sequence motifs of the hemotropic Mycoplasma 16S rRNA and RNase P genes indicate the presence of a novel organism. Hemotropic Mycoplasma spp. (hemoplasmas) are obligate epierythrocytic bacteria that infect numerous animal species, including Homo sapiens. Infections are often chronic and subclinical; however, some animals and humans develop hemolytic anemia, particularly when stressed or immunosuppressed (1, 2). Phylogenetic analyses of 16S rRNA gene sequences have defined two major subclusters of hemoplasmas, namely, the Mycoplasma haemosuis and Mycoplasma haemofelis groups (3-7).Historically, diagnosis of hemoplasma infections has relied on cytological examination of stained blood smears. In 1994, Dillberger and colleagues described Haemobartonella-like parasites in five wild-caught anemic cynomolgus monkeys (Macaca fascicularis) that originated from the Philippines; however, the organisms were not characterized phylogenetically (8). For some animal species, the diagnostic sensitivity of a blood smear examination is very poor and unspecific (3, 4, 9). The development of molecular assays, primarily targeting the 16S rRNA and the RNase P genes of these cell wall-deficient uncultivable microbes, has resulted in the recent recognition of several novel animal hemoplasmas (4, 5, 10-13).Bartonella spp. are facultative intracellular bacteria that also infect erythrocytes in numerous animal species, including Homo sapiens. Previously, Bartonella quintana DNA was amplified, cloned, and sequenced from lysed erythrocytes, and cultured colonies were grown from peripheral blood collected from a captivebred cynomolgus monkey (Macaca fascicularis) (14). Bartonella quintana was subsequently isolated from 2 of 36 captive rhesus macaques in China, of which 12 of 33 were B. quintana seroreactive (15).Hemotropic Mycoplasma and Bartonella organisms often cause persistent occult infection in immunocompetent hosts. The extent to which an infection with these bacteria in cynomolgus monkeys involved in a research study might influence assessments or outcomes associated with drug development studies is poorly characterized. This report describes PCR amplification and DNA sequence characterization of a novel hemotropic Mycoplasma sp. found in the blood of 44 of 52 cynomolgus research monkeys (Macaca fascicularis) and the isolation of Bartonella quintana from one monkey. These animals were in a chronic toxicity study, and data from the pretest phase of the study are presented. The animals were tested for Mycoplasma and Bartonella on the basis of the findings in a previous toxicity study that raised the possibility of latent infections. Based on the analysis of the 16S rRNA and RNase P gene sequences, we propose "Candidatus Mycoplasma haemomacaque" as the name for the novel hemotropic Mycoplasma sp. identified in this ...

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Section: Discussionmentioning

confidence: 99%

“…For some animal species, the diagnostic sensitivity of a blood smear examination is very poor and unspecific (3,4,9). The development of molecular assays, primarily targeting the 16S rRNA and the RNase P genes of these cell wall-deficient uncultivable microbes, has resulted in the recent recognition of several novel animal hemoplasmas (4,5,(10)(11)(12)(13).…”

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confidence: 99%

“Candidatus Mycoplasma haemomacaque” and Bartonella quintana Bacteremia in Cynomolgus Monkeys

et al. 2013

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“…Recently, a novel hemoplasma species was detected in capybaras (Hydrochaeris hydrochaeris) (VIEIRA et al, 2009). A total of 10 captive and 21 free-ranging animals were surveyed by PCR for hemoplasma infection.…”

Section: Wildlife Animalsmentioning

confidence: 99%

A review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in Brazil

Biondo¹,

Santos²,

Guimarães³

et al. 2009

RBPV

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Recent studies have been conducted in Brazil using molecular techniques for the detection of hemotrophic mycoplasmas in several mammals. In domestic cats, Mycoplasma haemofelis, 'Candidatus M. haemominutum', and 'Candidatus M. turicensis' infections have been identified. These species have also been found in free-ranging and captive neotropical felid species. Two canine hemoplasmas, Mycoplasma haemocanis and 'Candidatus Mycoplasma haematoparvum', have been identified in dogs. In commercial swine populations, Mycoplasma suis was found to be highly prevalent, especially in sows. Moreover, novel mycoplasma species have been identified in Brazilian commercial pigs and domestic dogs. A hemoplasma infection in a human patient infected with the human immunodeficiency virus (HIV) was also recently documented. In conclusion, hemoplasma species are common and important infectious agents in Brazil. Further studies should be conducted to better understand their impact on pets, production animals, and wildlife fauna, as well as their role as zoonotic agents, particularly in immunocompromised patients.Keywords: Hemoplasma, Mycoplasma haemofelis, Mycoplasma suis, HIV. ResumoEstudos recentes utilizando técnicas moleculares para a detecção de micoplasmas hemotróficos em diferentes mamíferos têm sido conduzidos no Brasil. Em gatos domésticos, infecções por Mycoplasma haemofelis, 'Candidatus M. haemominutum' e 'Candidatus M. turicensis' foram identificadas. Estas espécies também foram encontradas em felídeos neotropicais de vida livre e de cativeiro. Dois hemoplasmas caninos, Mycoplasma haemocanis e 'Candidatus Mycoplasma haematoparvum', foram identificados em cães domésticos. Em populações comerciais de suínos, Mycoplasma suis possui alta prevalência, especialmente em porcas. Além disso, novas espécies de hemoplasmas foram detectadas em suínos comercias e cães. Infecção por um hemoplasma em um paciente humano infectado com o vírus da imunodeficiência humana (HIV) foi recentemente documentada. Em conclusão, espécies de hemoplasmas são comuns e importantes agentes de infecções no Brasil. Estudos futuros devem ser conduzidos para melhor entender seu impacto em cães e gatos, animais de produção e na fauna silvestre, e também para determinar o seu papel como agentes zoonóticos, particularmente em pacientes imunocomprometidos.Palavras-chave: Hemoplasma, Mycoplasma haemofelis, Mycoplasma suis, HIV.

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“…Hemoplasmas são encontrados por todo mundo e no Brasil já foram identificados em gatos , cães , gado (Girotto et al, 2012), capivaras (Vieira et al, 2009), leões (Guimaraes et al, 2007) e veados (Grazziotin et al, 2011). Recentemente, o potencial zoonótico dos hemoplasmas foi reportado após a identificação molecular de hemoplasmas em um homem com imunossupressão e outro com exposição frequente a animais infectados por hemoplasmas (dos Santos et al, 2008;Steeret al, 2011;Sykes et al, 2010).…”

Section: Estudo Das Infecções Por Hemoplasmas Em Cães E Gatosunclassified

“…Haemoplasmas are found throughout the world and have previously been identified in cats , dogs , cattle (Girotto et al, 2012), capybaras (Vieira et al, 2009), lions (Guimaraes et al, 2007) and deer (Grazziotin et al, 2011) in Brazil. Recently, the zoonotic potential of haemoplasmas has been reported after the molecular identification of haemoplasmas in immunosuppressed humans and professionals, some with frequent exposure to haemoplasma infected animals (dos Santos et al, 2008;Steer et al, 2011;Sykes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Estudo das infecções por hemoplasmas em cães e gatos domésticos

Aquino¹

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vii AGRADECIMENTOSMeu maior obrigada a minha mãe, Lourdes, por ser não só mãe, mas também pai, amiga, torcedora, guerreira e porto seguro. Ela é meu principal exemplo de ser humano. Dedico e ofereço a ela todas minhas conquistas.Muito obrigada por ser minha maior incentivadora e, principalmente, por acreditar na minha capacidade, até quando eu mesma não acredito.Obrigada ao meu marido Darien por ser grande exemplo de otimismo, bondade e força, por me apoiar incondicionalmente e acreditar em mim.Obrigada pela vida que decidimos trilhar juntos e por tanto amor! Obrigada vó Diva, minha segunda mãe, pelos cuidados e orações.Elas têm funcionado! Obrigada aos meus irmãos, Eduardo, Marlos e Rovena, por toda oportunidade de aprendizado, por serem exemplos tão diferentes e se preocuparem comigo. Aos meus sobrinhos Guilherme e Felipe, que em tão pouco tempo já conquistaram tanto amor em nossos corações e pelas fofuras diárias! Obrigada pela amizade verdadeira, Patrícia, Bruna e Daniela! E por me ensinarem em todos os momentos de convívio e pelo apoio incondicional a qualquer hora! Obrigada Tatiana por todo apoio moral, técnico e intelectual de sua mente brilhante! Obrigada pela paciência e pelas inúmeras ajudas! Obrigada aos colegas de laboratório por toda ajuda e apoio.Meus agradecimentos a minha orientadora, Giane, pela oportunidade oferecida e pela orientação ao longo de tantos anos. Obrigada por ter acreditado no que eu podia oferecer e pela liberdade que tive ao longo dessa pesquisa.Obrigada à CAPES pelo apoio financeiro para que eu pudesse me dedicar exclusivamente a pesquisa e para que eu realizasse o doutorado viii sanduíche na Universidade de Bristol. Obrigada à FAPDF por financiar a apresentação desse projeto em congressos internacionais.Por fim, sou muito grata ao que o doutorado me proporcionou:conhecimento, descobertas, oportunidades e novos horizontes! Espero que esse conteúdo possa contribuir de alguma forma àqueles que se interessam pelas hemoplasmoses. ' (CMhp) are the most prevalent. The aims of the present study were to determine the occurrence and the phylogeny of haemoplasma species from cats in Brasilia and surrounding cities and from dogs in Nigeria, Africa; and assess weather any correlation existed between haemoplasma infection and haematological abnormalities. Blood samples were collected from 451 cats in different origins of Brasília, DF and from 246 dogs from Jos, Plateau State, Nigeria; submitted to erythrocyte evaluation and DNA extraction for PCR analysis and sequencing. In the cats study, haemoplasmas were found in 13.8% of 432 cats. CMhm was the most prevalent species (in 13.8% of cats), followed by Mhf (11.1%) and CMt (4.4%). Over 80% of haemoplasma-infected cats harboured two or more feline haemoplasma species: 7.1% of cats were coinfected with Mhf/CMhm, 0.4% with CMhm/CMt and 3.9% with Mhf/CMhm/CMt. Male gender was significantly associated with haemoplasma infections. No association was found between qPCR haemoplasma status and haematological variables, however CMhm relative copy nu...

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Detection of a novel hemoplasma based on 16S rRNA gene DNA in captive and free-ranging capybaras (Hydrochaeris hydrochaeris)

Cited by 31 publications

References 12 publications

“Candidatus Mycoplasma haemomacaque” and Bartonella quintana Bacteremia in Cynomolgus Monkeys

“Candidatus Mycoplasma haemomacaque” and Bartonella quintana Bacteremia in Cynomolgus Monkeys

A review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in Brazil

Estudo das infecções por hemoplasmas em cães e gatos domésticos

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