Detection of a novel microsporidium with intranuclear localization in farmed Penaeus vannamei from Latin America

Dhar, Arun K.; Cruz‐Flores, Roberto; Hùng, Nguyễn Hữu; Aranguren, Luis Fernando; Intriago, Pablo; Romero, Xavier

doi:10.1016/j.jip.2023.107968

Cited by 6 publications

(1 citation statement)

References 24 publications

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“…We decided to test different primers because of our experience that histological findings sometimes did not match the PCR results, e.g., the lack of correspondence between histology and PCR for intracellular bacteria, microsporidia and DHPV. We also tested different sets of primers such as those for the spore wall protein (SWP) gene of EHP, because of reports of cross reactions from existing PCR detection methods that target the EHP small subunit ribosomal RNA gene (Jaroenlak et al, 2016; Tang et al, 2017; Dhar et al 2023). We did tests with DNA from closely related microsporidia and form other aquatic organisms from different regions in Latin America.…”

Section: Introductionmentioning

confidence: 99%

Passive surveillance for shrimp pathogens inPenaeus vannameisubmitted from 3 Regions of Latin America

Intriago,

Medina,

Cercado

et al. 2023

Preprint

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Multiple PCR analyzes were performed using 19 different primer sets to open and broaden the search spectrum for shrimp pathogens. In addition, multiple primer pairs for 10 pathogens were compared to see if there were differences in selectivity or sensitivity among them. Some pathogens that did not present histological lesions were detected. The most important outcome was that selection of appropriate primers was the most critical factor in obtaining reliable results. We found high variability in results among primers and we learned it was prudent to seasonally assess among them for the best set selection. It is important to understand that a PCR positive test result alone does not confirm the presence of a viable pathogen or a disease state. Nor, as might be expected, does it mean that the positive PCR test results will be necessarily accompanied by histological lesions characteristic of the targeted pathogen. However, the use of appropriately selected primers sets can reveal whether there is an evolution in the result spectrum over time and if some pathogens disappear or reappear or new ones emerge. In general, most shrimp presented coinfections that consisted of the presence of WzSV8, DHPV, chronic midgut inflammation and tubule distension/epithelial atrophy consistent with Pir A/B toxicity. Also included were RLB/NHPB, microsporidia, striated muscle necrosis, gregarines in the hindgut caecum (gametocyte stage, and not associated with tegumental glands but glands that line the mouth and anus) and encysted, presumed nematode larvae. WzSV8 was newly discovered in gonads. Histological changes and the presence of spheroids in the lymphoid organ were considered as healthy host responses of often unidentified cause.

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Section: Introductionmentioning

confidence: 99%

Passive surveillance for shrimp pathogens inPenaeus vannameisubmitted from 3 Regions of Latin America

Intriago,

Medina,

Cercado

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Passive surveillance for shrimp pathogens in Penaeus vannamei submitted from 3 regions of Latin America

Intriago,

Medina,

Cercado

et al. 2024

Aquaculture Reports

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Detection of a novel microsporidium with intranuclear localization in farmed Penaeus vannamei from Latin America

Cited by 6 publications

References 24 publications

Passive surveillance for shrimp pathogens inPenaeus vannameisubmitted from 3 Regions of Latin America

Passive surveillance for shrimp pathogens inPenaeus vannameisubmitted from 3 Regions of Latin America

Passive surveillance for shrimp pathogens in Penaeus vannamei submitted from 3 regions of Latin America

Growth performance and growth model fitting of Litopenaeus vannamei cultured in pond and factory modes

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