Detection of a True Breeding Homeotic Gene Mutant Pps-1 with Partially Petaloid Sepals in Opium Poppy (Papaver somniferum L.) and Its Genetic Behavior

Dhawan, O. P.; Dubey, Mukesh; Khanuja, S. P. S.

doi:10.1093/jhered/esm037

Cited by 10 publications

(10 citation statements)

References 27 publications

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“…Previously, the segregation of Pps-1 mutation at a ratio of 3∶1 has been shown [20], confirming the involvement of single recessive gene in regulating the partially petaloid character. For segregation analysis of the second mutant, the parent genotype I-268 having normal white petals was crossed with OM mutant having sepaloid petals.…”

Section: Methodsmentioning

confidence: 69%

“…In the present investigation we analyzed different genes involved in flower development by utilizing partially petaloid sepal (Pps-1) and sepaloid petal (OM) mutants that were obtained from the normal sepal and petal phenotypes of I-14 and I-268, respectively. The development of Pps-1 has been described earlier [20], [21]. In the Pps-1 mutant, a part of the sepal is converted into petal rather than forming a complete sepal ( Figure 1 ) whereas in OM the whole petal is converted into a sepal ( Figure 2 ).…”

Section: Introductionmentioning

confidence: 80%

“…The parent genotype I-14 is characterized by narrow leaves with very deep leaf incisions and white flower petals [20]. In Pps-1, the margins of the sepals are modified into petal-like characters ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

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Recessive Loci Pps-1 and OM Differentially Regulate PISTILLATA-1 and APETALA3-1 Expression for Sepal and Petal Development in Papaver somniferum

et al. 2014

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The involvement of PISTILLATA (PI) and APETALA (AP) transcription factors in the development of floral organs has previously been elucidated but little is known about their upstream regulation. In this investigation, two novel mutants generated in Papaver somniferum were analyzed - one with partially petaloid sepals and another having sepaloid petals. Progeny from reciprocal crosses of respective mutant parent genotypes showed a good fit to the monogenic Mendelian inheritance model, indicating that the mutant traits are likely controlled by the single, recessive nuclear genes named “Pps-1” and “OM” in the partially petaloid sepal and sepaloid petal phenotypes, respectively. Both paralogs of PISTILLATA (PapsPI-1 and PapsPI-3) were obtained from the sepals and petals of P. somniferum. Ectopic expression of PapsPI-1 in tobacco resulted in a partially petaloid sepal phenotype at a low frequency. Upregulation of PapsPI-1 and PapsAP3-1 in the petal and the petal part of partially petaloid sepal mutant and down-regulation of the same in sepaloid petal mutant indicates a differential pattern of regulation for flowering-related genes in various whorls. Similarly, it was found that the recessive mutation OM in sepaloid petal mutant downregulates PapsPI-1 and PapsAP3-1 transcripts. The recessive nature of the mutations was confirmed by the segregation ratios obtained in this analysis.

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Section: Methodsmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 80%

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Recessive Loci Pps-1 and OM Differentially Regulate PISTILLATA-1 and APETALA3-1 Expression for Sepal and Petal Development in Papaver somniferum

et al. 2014

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“…The significantly higher degree of variation in selected 35 accessions of opium poppy for individual traits was confirmed by multivariate (D 2 ) and canonical analysis based on the pooling of 10 yield contributing characters in point scale. The genotypes were (Dhawan et al 2007) and both the genotypes were found to be near isogenic lines. The cluster VII contained genotypes (I-266 and I-344) which were found to be short in height.…”

Section: Genetic Divergence Studies and Strategies For Crop Improvementmentioning

confidence: 95%

Downy mildew resistance in opium poppy: resistance sources, inheritance pattern, genetic variability and strategies for crop improvement

2008

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Downy mildew (DM) caused by Peronospora arborescens is the most destructive disease of opium poppy which assumes considerable importance in India and other poppy growing countries. The present study was aimed at identification and evaluation of stable resistance sources of DM in opium poppy. Furthermore, genetic variability and inheritance pattern of DM resistance has also been studied which can help in making strategy for crop improvement. Evaluation of 35 selected germplasm accessions of opium poppy under glasshouse and field conditions during the three consecutive years (

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“…The information on homeotic mutants of other nonmodel species also continues to accumulate (for example see Smith-Huerta 1992;Webster and Gilmartin 2003;Dhawan et al 2007).…”

Section: Homeotic Transformations In Tlb and The Petaloidy Of Floral mentioning

confidence: 99%

Genetic and morphological analysis of floral homeotic mutantstepal-like bractandfagopyrum apetalaofFagopyrum esculentum

Logacheva

Fesenko

Фесенко

et al. 2008

Botany

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The studies on floral homeotic mutants of the model plant species Arabidopsis thaliana (L.) Heynh. and Antirrhinum majus L. have clarified many important aspects of the genetic control of flower development. However, the details of this process can vary in species representing different lineages of flowering plants. The studies on floral homeotic mutants of nonmodel plant species may significantly improve the understanding of the mechanisms of morphological evolution of flowers. We report here the results of the genetic and morphological analysis of two floral homeotic mutants of common buckwheat (Fagopyrum esculentum Moench.). The mutant, tepal-like bract (tlb), is characterized by the transformation of bracts into petaloid organs, whereas fagopyrum apetala (fap), has a carpelloid perianth. Both mutant phenotypes are caused by a single recessive nuclear mutation. The double mutant fap tlb combines the features of tlb and fap. Our results show that single gene mutations are sufficient to convert the buckwheat bract into a tepal and to confer carpel identity on first whorl organs. These results are consistent with the premise that variations on the ABC model can be used to explain a wide range of floral architectures.Résumé : L'étude de mutants floraux homéotiques des plantes modèles Arabidopsis thaliana (L.) Heynh. et Anthirrhinium majus L. ont clarifié plusieurs aspects du contrôle génétique du développement floral. Cependant, les détails de ce processus peuvent varier selon les espèces représentant différentes lignées de plantes à fleurs. Les études sur les mutants floraux homéotiques portant sur des espèces de plantes non-modèles peuvent améliorer significativement la compréhension des mécanismes de l'évolution morphologique des fleurs. Les auteurs rapportent les résultats de l'analyse morphologique et gé-nétique de deux mutants floraux homéotiques du sarrasin (Fagopyrum esculentum Moench.). Le mutant, bractées tépaloï-des (tlb), se caractérise par la transformation des bractées en organes pétaloïdes, alors que le fagopyrum apetala (fap) possède un périanthe carpelloïde. Ces deux phénotypes mutants sont causés par une mutation nucléique récessive unique. Le double mutant fap tlb combine les caractéristiques des tlb et des fap. Les résultats montrent que des mutations à gène unique suffisent pour convertir la bractée du sarrasin en tépale et à conférer une identité de carpelle au premier verticille d'organes. Ces résultats concordent avec la prémisse que les variations sur le modèle ABC peuvent être utilisées pour expliquer un ensemble d'architectures florales.

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Detection of a True Breeding Homeotic Gene Mutant Pps-1 with Partially Petaloid Sepals in Opium Poppy (Papaver somniferum L.) and Its Genetic Behavior

Cited by 10 publications

References 27 publications

Recessive Loci Pps-1 and OM Differentially Regulate PISTILLATA-1 and APETALA3-1 Expression for Sepal and Petal Development in Papaver somniferum

Recessive Loci Pps-1 and OM Differentially Regulate PISTILLATA-1 and APETALA3-1 Expression for Sepal and Petal Development in Papaver somniferum

Downy mildew resistance in opium poppy: resistance sources, inheritance pattern, genetic variability and strategies for crop improvement

Genetic and morphological analysis of floral homeotic mutantstepal-like bractandfagopyrum apetalaofFagopyrum esculentum

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