Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

Hasmiwati, Hasmiwati; Rusjdi, Selfi Renita; Nofita, Eka

doi:10.13057/biodiv/d190105

Cited by 20 publications

(21 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aegypti populations in India and Indonesia has also been suggested. 82 , 83 However, it is intriguing that for the mutations reported in Aedes mosquitoes, only a small difference in the inhibition profile of ACE has been suggested, which is different from the impacts of similar mutations observed in Culex and Anopheles species. The present study detected changes in total ACE activity (AChE) in 17% of the analysed samples, but in no case did the remaining ACE activity after insecticide inhibition (AChI) signal the alteration of this endpoint.…”

Section: Discussionmentioning

confidence: 99%

Resistance to temephos and deltamethrin in Aedes aegypti from Brazil between 1985 and 2017

Valle

Bellinato

Viana-Medeiros

et al. 2019

Mem. Inst. Oswaldo Cruz

116

View full text Add to dashboard Cite

BACKGROUND Aedes aegypti populations in Brazil have been subjected to insecticide selection pressures with variable levels and sources since 1967. Therefore, the Brazilian Ministry of Health (MoH) coordinated the activities of an Ae. aegypti insecticide resistance monitoring network (MoReNAa) from 1999 to 2012. OBJECTIVES The objective of this study was to consolidate all information available from between 1985 and 2017 regarding the resistance status and mechanisms of Brazilian Ae. aegypti populations against the main insecticide compounds used at the national level, including the larvicide temephos (an organophosphate) and the adulticide deltamethrin (a pyrethroid). METHODS Data were gathered from two sources: a bibliographic review of studies published from 1985 to 2017, and unpublished data produced by our team within the MoReNAa between 1998 and 2012. A total of 146 municipalities were included, many of which were evaluated several times, totalling 457 evaluations for temephos and 274 for deltamethrin. Insecticide resistance data from the five Brazilian regions were examined separately using annual records of both the MoH supply of insecticides to each state and the dengue incidence in each evaluated municipality. FINDINGS Ae. aegypti resistance to temephos and deltamethrin, the main larvicide and adulticide, respectively, employed against mosquitoes in Brazil for a long time, was found to be widespread in the country, although with some regional variations. Comparisons between metabolic and target-site resistance mechanisms showed that one or another of these was the main component of pesticide resistance in each studied population. MAIN CONCLUSIONS (i) A robust dataset on the assessments of the insecticide resistance of Brazilian Ae. aegypti populations performed since 1985 was made available through our study. (ii) Our findings call into question the efficacy of chemical control as the sole methodology of vector control. (iii) It is necessary to ensure that sustainable insecticide resistance monitoring is maintained as a key component of integrated vector management. (iv) Consideration of additional parameters, beyond the supply of insecticides distributed by the MoH or the diverse local dynamics of dengue incidence, is necessary to find consistent correlations with heterogeneous vector resistance profiles.

show abstract

Section: Discussionmentioning

confidence: 99%

Resistance to temephos and deltamethrin in Aedes aegypti from Brazil between 1985 and 2017

Valle

Bellinato

Viana-Medeiros

et al. 2019

Mem. Inst. Oswaldo Cruz

116

View full text Add to dashboard Cite

show abstract

“…Both the non-synonymous mutation G119S (GGC/AGC) and silent mutation T506T on ace-1 [42] have not been detected in our sequences in Ae. aegypti samples from Jeddah.…”

Section: Discussionmentioning

confidence: 57%

“…aegypti, the primer set IIS5-6(3)F and IIS5-6(3)R were used to amplify the IIS6 domain of the VGSC, spanning the codons 989, 1011, 1016, and the introns between exons 20 and 21; while the codon 1534 domain IIIS6 was ampli ed by the primer set AaNa31F and AaNa31R [41]. The ace-1 gene region spanning the position 119 was ampli ed by the primer set Ace-1F and Ace-1R [42]. For Cx.…”

Section: Dna Extraction and Target Gene Mutation Sequencingmentioning

confidence: 99%

Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia

Fang¹,

Ernest²,

Xue³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Gene mutations on target sites can be a valuable reference to the status of insecticide resistance. Jeddah, a global commercial and major port-of-entry city, is bearing the most (>70%) dengue disease burden and the population of the dengue vector Aedes aegypti (Linnaeus, 1762) in Saudi Arabia. Culex quinquefasciatus Say, 1823 is a second example as one of the major arboviral vectors in the region. However, the status of insecticide resistance and targeted site mutations on the responsible genes are not fully characterized.Methods: We randomly sampled both mosquito species, Ae. aegypti (25 individuals) and Cx. quinquefasciatus (24 individuals) across Jeddah by daily mosquito surveillance in 2016 to detect the resistance-associated target site mutations on the voltage gated sodium channel (VGSC) and acetylcholinesterase 1 (ace-1) genes by PCR amplification and direct sequencing. Results: Our findings showed that Ae. aegypti resistance-associated VGSC gene mutations revealed polymorphic mutations on the 989, 1016, and 1534 sites. Additionally, we documented two types of introns between exons 20 and 21, however, the I1011M point mutation was undetected. Linkage disequilibrium associations were shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. Furthermore, no mutation on ace-1 was identified in Ae. aegypti. In Cx. quinquefasciatus, homozygous L1014F/L1014F (95.23%) on the VGSC and heterozygous G119/G119S (100%) on ace-1 were widely distributed in the samples studied. Analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (P = 0.000) allele type, and may reflect the history of insecticide treatment in different continents.Conclusions: To the best of our knowledge, this is the first record of the intron types between exons 20 and 21 on VGSC of Ae. aegypti populations. It can serve as a reference marker to evaluate the local insecticide resistance status. In addition, the first report of insecticide resistance gene mutation being present in field caught Cx. quinquefasciatus in Saudi Arabia. High prevalence of insecticide resistance gene mutations in local primary mosquito vector species alert the urgent need to carry out a comprehensive insecticide resistance surveillance.

show abstract

“…Both the non-synonymous mutation G119S (GGC/AGC) and silent mutation T506T on ace-1 [43] were not detected in our sequences in Ae. aegypti samples from Jeddah.…”

Section: Discussionmentioning

confidence: 59%

“…aegypti, the primer set IIS5-6(3)F and IIS5-6(3)R were used to amplify the IIS6 domain of the VGSC, spanning the codons 989, 1011, 1016, and the introns between exons 20 and 21; while the codon 1534 domain IIIS6 was ampli ed by the primer set AaNa31F and AaNa31R [42]. The ace-1 gene region spanning the position 119 was ampli ed by the primer set Ace-1F and Ace-1R [43]. For Cx.…”

Section: Dna Extraction and Target Gene Mutation Sequencingmentioning

confidence: 99%

Molecular Analysis of Targeted Insecticide Resistance Gene Mutations in field Aedes Aegypti and Culex Quinquefasciatus Mosquito Vectors of Arboviruses from Saudi Arabia

Fang

Tambo²,

Xue³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background In absence of effective and safe vaccines or drugs against mosquito-borne arboviral diseases (such as dengue, Zika and Rift Valley and West Nile), there have been an increasing pattern of insecticide use against the mosquito vectors of these diseases in integrated vector management (IVM) programs in Saudi Arabia. However, the efficacy of IVM programmes is threatened by the development of insecticide resistance in vectors. Gene mutations on target sites can be a valuable reference to the status of insecticide resistance. Jeddah, a global commercial and major port-of-entry city, is bearing the most (> 70%) dengue disease burden and the population of the dengue vector Aedes aegypti in Saudi Arabia. Culex quinquefasciatus is a second example as one of the major arboviral vectors in the region and a proven vector of Rift Valley fever and wide-spread in Jeddah and the rest of the western region of Saudi Arabia. However, the status of insecticide resistance and targeted site mutations on the responsible genes are not fully characterized.Methods We randomly sampled both mosquito species, Ae. aegypti and Cx. quinquefasciatus across Jeddah by daily mosquito surveillance in 2016 to detect the resistance-associated target site mutations on the voltage gated sodium channel (VGSC) and acetylcholinesterase 1 (ace-1) genes.Results Our findings showed that Ae. aegypti resistance-associated VGSC gene mutations revealed polymorphic mutations on the 989 (allele frequency = 65.91%), 1016 (allele frequency = 65.91%), and 1534 (allele frequency = 52.27%) sites. Additionally, we documented two types of introns between exons 20 and 21, however, the I1011M point mutation was undetected. Linkage disequilibrium associations were shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. Furthermore, no mutation on ace-1 was identified in Ae. aegypti. In Cx. quinquefasciatus, homozygous L1014F/L1014F (95.23%) on the VGSC and heterozygous G119/G119S (100%) on ace-1 were widely distributed in the samples studied.Conclusions To the best of our knowledge, this is the first record of the intron types between exons 20 and 21 on VGSC of Ae. aegypti populations, and the first report of insecticide resistance gene mutation being present in field caught Cx. quinquefasciatus in Jeddah, Saudi Arabia. High prevalence of insecticide resistance gene mutations in local primary mosquito vector species, implies that the local Ae. aegypti mosquitoes are resistant to both pyrethroid and DDT, while the resistance to all four insecticide groups may possessed in Cx. quinquefasciatus populations. Our findings alert the urgent need to carry out a comprehensive insecticide resistance gene mutation surveillance and monitoring to guide sustained and effective IVM planning and innovative guidelines in local predominate mosquito populations and mosquito-borne disease control and elimination in Saudi Arabia.

show abstract

Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

Cited by 20 publications

References 21 publications

Resistance to temephos and deltamethrin in Aedes aegypti from Brazil between 1985 and 2017

Resistance to temephos and deltamethrin in Aedes aegypti from Brazil between 1985 and 2017

Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia

Molecular Analysis of Targeted Insecticide Resistance Gene Mutations in field Aedes Aegypti and Culex Quinquefasciatus Mosquito Vectors of Arboviruses from Saudi Arabia

Contact Info

Product

Resources

About