Detection of active oxygen generated from ceramic powders having antibacterial activity.

Sawai, Jun; Kawada, Emiko; Kanou, Fumio; Igarashi, Hideo; Hashimoto, Atsushi; Kokugan, Takao; Shimizu, Masaru

doi:10.1252/jcej.29.627

Cited by 301 publications

(193 citation statements)

References 23 publications

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“…Previous studies by our group showed that active oxygen species were generated from CaO Sawai et al, 1996 andHSSP Kubo et al, 2013 , so we NBRC1950 growth with the addition of HESP. Similar measurements were conducted for other fungi and bacteria.…”

Section: Active Oxygen Generated From Hespmentioning

confidence: 92%

Antimicrobial Characteristics of Heated Eggshell Powder

et al. 2015

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Eggshells have high bioavailability and can be used as a source of calcium. The main component is CaCO 3 , which, when heated, is converted to CaO. Seashells are also mainly composed of CaCO 3 and were previously found to exhibit antimicrobial activity after being heated. In this study, heated eggshell powder HESP was found to have antimicrobial activity against bacterial vegetative cells, fungi and bacterial spores. Parameters, such as the minimum inhibitory concentration, were determined with kinetic analysis using an indirect conductimetric assay. Moreover, HESP was able to kill the Bacillus subtilis spores. There were no significant differences in the activity between HESP, heated scallop-shell powder and pure CaO. The MIC values for HESP against bacteria and fungi were 0.29-0.43 and 1.3-1.5 mg/mL, respectively. Against B. subtilis spores, a reduction of two orders of magnitude of viability was confirmed following 20 min of treatment at 10 mg/mL at 60 . The active oxygen generated from the HESP slurry was examined with chemiluminescence. The intensity of this increased with increasing concentrations of the HESP slurry. This suggests that HESP could be used as a natural antimicrobial agent. Although a high pH is the main contributor to this antimicrobial activity, active oxygen species generated from HESP are likely to be the main antimicrobial agents..

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Section: Active Oxygen Generated From Hespmentioning

confidence: 92%

Antimicrobial Characteristics of Heated Eggshell Powder

et al. 2015

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“…The conductimetric assay yielded identical results. In our previous study [26], active oxygen species were generated from CaO, the main constituent of HSSP. We therefore investigated whether active oxygen species were generated from the HSSP slurry.…”

Section: Cells (Red) Most Of the E Coli Cells In This Biofilmmentioning

confidence: 99%

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7

Kubo¹,

Ohshima²,

Irie³

et al. 2013

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The ability of heated scallop-shell powder (HSSP) to disinfect Escherichia coli ATCC 25922 biofilm was investigated. On account of its cryotolerance and cell surface characteristics, the E. coli strain is reportedly a useful surrogate for E. coli O157: H7 in surface attachment studies. In this study, an E. coli ATCC 25922 biofilm was formed on a glass plate, and immersed in a slurry of HSSP. Following treatment, the disinfection ability of the HSSP toward the biofilm was non-destructively and quantitatively measured by conductimetric assay. The disinfection efficacy increased with HSSP concentration and treatment time. HSSP treatment (10 mg/mL, pH 12.5) for 20 min completely eliminated biofilm bioactivity (approximately 10 8 CFU/cm 2 in non-treated biofilms). In contrast, treatment with NaOH solution at the same pH, and treatment with sodium hypochlorite (200 g/mL) reduced the activity by approximately one to three log 10 . Fluorescence microscopy confirmed that no viable cells remained on the plate following HSSP treatment (10 mg/mL). Although alkaline and sodium hypochlorite treatments removed cells from the biofilm, under these treatments, many viable cells remained on the plate. To elucidate the mechanism of HSSP activity against E. coli ATCC 25922, the active oxygen generated from the HSSP slurry was examined by chemiluminescence analysis. The luminescence intensity increased with increasing concentration of HSSP slurry. The results suggested that, besides being alkaline, HSSP generates active oxygen species with sporicidal activity. Thus, HSSP treatment could also be effective for controlling biofilms of the toxic strain E. coli O157: H7, implicated in food poisoning.

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“…Moreover, it can be applied to treat different skin conditions, in products like baby powder and creams against diaper rashes, antiseptic ointments, anti-dandruff shampoos, and as a component in tape (called ''Zinc oxide tape'') used by athletes as a bandage to prevent soft tissue damage during workouts. Swai et al (1996) stated that the inorganic compound ZnO exhibits strong resistance against microorganisms. The advantage of using ZnO as antimicrobial agent is due to the fact that its mineral elements are essential to humans and also exhibit strong activity even when asmall amount is administrated into human body.…”

Section: Introductionmentioning

confidence: 99%

Characterization and study of antibacterial activity of spray pyrolysed ZnO:Al thin films

et al. 2015

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Aluminum-doped zinc oxide (ZnO:Al) thin films were deposited onto glass substrates using spray pyrolysis technique with the substrate temperature of 400°C. X-ray diffraction analysis indicated that the films were polycrystalline with hexagonal wurtzite structure preferentially oriented along (002) direction. Surface morphology of the films obtained by scanning electron microscopy showed that the grains were of nanoscale size with porous nature for 6 at.% of Al. Atomic force microscopy observations revealed that the particles size and surface roughness of the films decreased with Al-doping. Optical measurements indicated that ZnO:Al (6 at.%) exhibited a band gap of 3.11 eV, which is lower than that of pure ZnO film, i.e. 3.42 eV. Photoluminescence analysis showed weak NBE emission at 396 nm for Al-doped films. The low resistivity, high hall mobility and carrier concentration values were obtained at a doping ratio of 6 at.% of Al. The effective incorporation of 6 at.% of Al into ZnO lattice by occupying Zn sites yielded a well-pronounced antibacterial activity against Staphylococcus aureus.

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Detection of active oxygen generated from ceramic powders having antibacterial activity.

Cited by 301 publications

References 23 publications

Antimicrobial Characteristics of Heated Eggshell Powder

Antimicrobial Characteristics of Heated Eggshell Powder

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7

Characterization and study of antibacterial activity of spray pyrolysed ZnO:Al thin films

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Detection of active oxygen generated from ceramic powders having antibacterial activity.

Cited by 301 publications

References 23 publications

Antimicrobial Characteristics of Heated Eggshell Powder

Antimicrobial Characteristics of Heated Eggshell Powder

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of &lt;i&gt;Escherichia coli&lt;/i&gt; ATCC 25922 Surrogated for &lt;i&gt;E. coli&lt;/i&gt; O157:H7

Characterization and study of antibacterial activity of spray pyrolysed ZnO:Al thin films

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Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7