Detection of Aeromonas salmonicida from water using a filtration method

Ford, Larisa A.

doi:10.1016/0044-8486(94)90328-x

Cited by 17 publications

(4 citation statements)

References 12 publications

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“…Dashed horizontal line : limit of enumeration in water ammonium acetate precipitation followed by further purification using SpinBind columns was found to be highly effective for removal of humic contaminants. Detection limits of less than 1 cfu ml-' water have been reported using non-selective media (Maheshkumar et al 1990;Ford 1994) but for the samples used in this study the limits were more in agreement with those found by Morgan et al (1993) of around 300 cfu ml-'. Even with the aid of catechol spraying, accurate enumeration on permissive media was not possible at cell densities of < lo4 ml-' (day 3 onwards) due to overgrowth by the indigenous microflora.…”

Section: Genome Numbers In Sediment Layerssupporting

confidence: 89%

Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

Deere

Porter

Pickup

et al. 1996

Journal of Applied Bacteriology

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The survival of the bacterial fish pathogen Aeromonas salmonicida, and persistence of its DNA, were monitored in aquatic microcosms using selective culture and most probable number PCR. Bacterial cells and naked DNA were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. Two different types of surface sediment were used. One was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. Inoculated cells and naked DNA became undetectable from water overlayers within 4 weeks of release. Colony counts of Aer. salmonicida declined below detectable limits after 4 weeks in loamy sediment or 7 weeks in sandy sediment; however, naked DNA and DNA from released cells remained detectable for more than 13 weeks.

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Section: Genome Numbers In Sediment Layerssupporting

confidence: 89%

Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

Deere

Porter

Pickup

et al. 1996

Journal of Applied Bacteriology

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“…This research was originally motivated by a need for nonlethal sampling procedures to evaluate disease status in valuable threatened or endangered fish. Procedures developed by Ford (1994) to isolate A. salmonicida from the water and those de- scribed by Cipriano et al (1992) to isolate the pathogen from mucus enable hatchery biologists to detect A. salmonicida at early states of infection. When incorporated into a regular fish health monitoring program, these procedures provide biologists with proactive tools to minimize the deleterious effects of disease.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteria were quantified in dilutions containing 10-30 colony-forming units (cfu). In addition, water samples from the White River (the source of water for the culture units) were processed weekly for the presence of the bacterium as described by Ford (1994). Individual bacterial colonies derived from water and fish samples were subcultured onto tryptic soy agar (Difco Laboratories, Detroit.…”

Section: Methodsmentioning

confidence: 99%

Monitoring for Early Detection ofAeromonas salmonicidato Enhance Antibiotic Therapy and Control Furunculosis in Atlantic Salmon

Cipriano

Ford

Nelson

et al. 1996

The Progressive Fish-Culturist

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“…Les analyses bactériologiques ont été faites suivant la technique des membranes filtrantes (HAVELAAR ef al., 1987 ;FORD, 1994), en utilisant des membranes en ester de cellulose -Millipore, Bedford, MA 01730 -de porosité 0,45 nm. La recherche et l'isolement de P. aeruginosa ont été faits sur milieu Pyocyanosel (MARCHAL et al, 1991).…”

Section: Prélèvement Et Analyse Des éChantillonsunclassified

Distribution de Pseudomonas aeruginosa et Aeromonas hydrophila dans les eaux de la nappe phréatique superficielle en zone équatoriale au Cameroun et relations avec quelques paramètres chimiques du milieu.

Nola¹,

Njiné²,

Sikati³

et al. 2005

rseau

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Une étude microbiologique et chimique a été menée pendant un an sur les eaux de sources et de puits de Yaoundé (Cameroun). Les analyses microbiologiques ont été faites suivant la technique des membranes filtrantes, et les analyses chimiques, suivant les techniques analytiques usuelles. Les abondances maximales mensuelles de Pseudomonas aeruginosa et de Aeromonas hydrophila varient respectivement de 1 à 22x103 UFC.100 ml-1 d'eau, et de 1 à 7,8x103 UFC.100 ml-1. Ces abondances bactériennes subissent d'amples fluctuations spatio-temporelles. Les eaux de sources et de puits analysées sont faiblement bicarbonatées, douces et présentent une minéralisation faible à moyenne. Le pH varie de 3 à 5 et les concentrations en CO2 dissous, de 300 à 532 mg.l-1. Un bon nombre des caractéristiques chimiques de ces eaux est relativement stable au cours du temps, en dépit des fluctuations spatiales apparentes. Le degré de corrélation entre les paramètres chimiques et la dynamique d'abondance des bactéries isolées, est hétérogène. Cela est nettement plus marqué dans les eaux de puits, en raison des conséquences de l'exploitation humaine de ces eaux, à l'origine d'apports allochtones divers.

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Detection of Aeromonas salmonicida from water using a filtration method

Cited by 17 publications

References 12 publications

Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

Monitoring for Early Detection ofAeromonas salmonicidato Enhance Antibiotic Therapy and Control Furunculosis in Atlantic Salmon

Distribution de Pseudomonas aeruginosa et Aeromonas hydrophila dans les eaux de la nappe phréatique superficielle en zone équatoriale au Cameroun et relations avec quelques paramètres chimiques du milieu.

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