Detection of aflatoxin M1 in cheese samples by ELISA

Sarimehmetoğlu, Belgin; Küplülü, Özlem; Çelik, Tarık Halûk

doi:10.1016/s0956-7135(03)00006-9

Cited by 99 publications

(77 citation statements)

References 15 publications

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“…Therefore, AFM 1 level were low in the milk samples. These results did not support several previous studies that were performed in the same region or other regions of Turkey [12][13][14][15] , which reported that the milk samples contained more than 50 ng/kg of AFM 1 .…”

contrasting

confidence: 99%

Determination of Various Mycotoxin Concentrations in the Feedstuffs and Feed Produced by A Feed Manufacturer in Turkey

Oruç¹,

Sorucu²,

Turkmen³

et al. 2012

Kafkas Univ Vet Fak Derg

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SummaryThe aim of this study was to determine aflatoxin B1 (AFB1), T-2 toxin, fumonisin, deoxynivalenol (DON) and zearalenone (ZEN) levels in various feedstuffs (local and imported feed materials) and feed produced in a feed manufacturer. In addition, aflatoxin M1 (AFM1) levels were determined in raw cow milks (n = 24) collected from 10 certain dairy cattle farms. The samples consisted of dairy cattle (n = 26), fattening (n = 4), and calf starter (n = 4) feed, as well as feedstuffs (n = 40). All of the samples were analysed by ELISA. In the analysed samples, the incidence of AFB1, AFM1 and T-2 toxins, fumonisin, DON and ZEN were 100%, 80%, 75% and 33%, respectively. The results of this study in feedstuffs and feed revealed that the mycotoxin levels were lower than the mycotoxin limits of the European Union (EU) and Turkey. In previous studies, high concentrations of mycotoxins, especially AFB1 and AFM1, were identified in feedstuffs, feeds and milk in Turkey. In contrast, the mycotoxin levels in the current study were lower than that from the previous studies. The mycotoxin concentrations in imported feed materials (except fumonisin) were lower than those in feed materials obtained from Turkey. The results of this study indicate that the mean mycotoxin levels in feedstuff and feeds do not cause toxic effects in animals, and humans for AFM1. Keywords: Feedstuffs, Feed, Feed manufacturer, Mycotoxin levels, Multiple mycotoxin Bir Yem Fabrikası Tarafından Türkiye'de Üretilen Yem ve Yem Hammaddelerinde Çeşitli Mikotoksinlerin Miktarlarının Belirlenmesi ÖzetBu çalışmanın amacı bir yem fabrikası tarafından üretilen yemlerde ve çeşitli yem hammaddelerinde (yerli ve ithal) aflatoksin B1 (AFB1), T-2 toksin, fumonisin, deoksinivalenol (DON) ve zearalenone (ZEN) düzeylerinin belirlenmesidir. Ayrıca, seçilen 10 farklı süt işletmesinden toplanan inek sütlerinde (n = 24) aflatoksin M1 (AFM1) miktarlarının belirlenmesi amaçlanmıştır. Numuneler, süt yemi (n = 26), besi yemi (n = 4) ve buzağı başlangıç yemi (n = 4), ile yem hammaddelerinden (n = 40) oluşmaktadır. Mikotoksin miktarları ELISA ile analiz edildi. Mikotoksinlerin tespit edilme oranı AFB1, AFM1 ve T-2 toksin, fumonisin, DON ve ZEN için sırasıyla %100, %80, %75 ve %33'tür. Yem ve yem hammaddelerinde bulduğumuz mikotoksin miktarlarının Türkiye ve Avrupa Birliği'nde uygulanan limitlerin altında olduğu görüldü. Türkiye'de süt, yem ve yem hammaddelerinde daha önce yapılan çalışmalarda özellikle AFB1 ve AFM1 limitlerin üzerinde tespit edilmiştir. Bu çalışmanın sonuçları benzer olan önceki çalışmaların sonuçlarının aksine daha düşüktür. İthal yem hammaddeleri Türkiye'den sağlanan yem hammaddelerinden (fumonisin hariç) daha düşük miktarlarda mikotoksin içermektedir. Bu çalışmanın sonuçlarına göre, yem ve hammaddelerindeki ortalama mikotoksin düzeylerinin hayvan sağlığı; sütlerdeki AFM1 miktarlarının da insan sağlığı açısından toksik bir etkiye neden olamayacağı görüldü.

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contrasting

confidence: 99%

Determination of Various Mycotoxin Concentrations in the Feedstuffs and Feed Produced by A Feed Manufacturer in Turkey

Oruç¹,

Sorucu²,

Turkmen³

et al. 2012

Kafkas Univ Vet Fak Derg

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“…Furthermore, the results of the present study were observed to be in parallel with another study conducted by Karakaya and Atasever (2010) Egmond, 1989;Wood, 1991;Bakırcı, 2001;Özkaya et al, 2002;Akdemir and Altıntaş, 2004;Kamkar, 2005). Due to the concentrate feed either being added at lower levels or not being incorporated into the feed ration of dairy animals in summer (Wood, 1991;Sarımehmetoğlu et al, 2003) and in view of dairy cattle consuming greater amounts of compound feed in winter and spring compared to summer and autumn, it has been reported that higher levels of AFLM1 are indicated in milk during the cold seasons, compared to the warm seasons.…”

supporting

confidence: 87%

Aflatoxin Levels in Roughage, Concentrates, Compound Feed and Milk Samples from Dairy Farms in Erzurum Province

Polat

Gül

2014

Atatürk Üniversitesi Vet. Bil. Derg.

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Aflatoxin in roughage, concentrates and compound feed from dairy farms located in Erzurum province, and the presence of Aflatoxin M1 (AFLM1) in the milk of animals fed with these feeds were determined in four different seasons. The mean level of Aflatoxin M1 detected in milk samples was 0.03 ppb. Aflatoxin M1 levels in the milk samples taken from the holdings were lower in autumn and summer (0.02 ppb) compared to winter and spring (0.04 ppb). The total aflatoxin levels in feed samples were higher in spring and summer, compared to autumn and winter. Furthermore, Aflatoxin B1 (AFLB1) levels in roughage, concentrate and compound feed were significantly higher in spring and summer, compared to autumn and winter (P<0.01). The results obtained in this study demonstrated that Aflatoxin B1 received with compound feed by cattle led to a carry-over of Aflatoxin M1 into milk at a level of 1.219±0.4139%. The mycotoxin levels detected were below the maximum residue limits laid down in the Turkish Food Codex.

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“…Authors in 5 reported 96.25% of the yoghurt samples (40 industrial, 40 traditional) were contaminated with AFM1 in concentration levels ranging from <5 to 91ng/kg. According to 9 , in Ankara detected AFM1 contamination in 62.88% yoghurt samples, ranging from 50-800ng/kg. In another study 10 , in Turkey, 177 yoghurt samples (104 ordinary yoghurt, 21 fruit yoghurtand 52 of strained (Torba) yoghurt) were tested for AFM1 by ELISA method.…”

Section: Resultsmentioning

confidence: 99%

Aflatoxin M1 Contamination in Traditional Yoghurts Produced in Guilan Province, Iran

Nikbakht¹,

Lachiniyan²,

Rahbar³

et al. 2016

AJPRHC

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Aflatoxin M1 is a major carcinogenic compound that may be existed in dairy products. The aim of this study was to determine the occurrence of AFM1 in traditional yoghurt samples in Guilan Province (Northern Iran). Ninety samples of traditional yoghurts were collected during summer and autumn 2014. Enzyme linked Immunosorbentassay (ELISA) which is a rapid and sensitive method was used to determine the presence and levels of AFM1. 100% of the yoghurt samples were contaminated with 5 and 83 ng/kg of AFM1. In general, AFM1 in 20 samples (22.22%) were higher than the maximum tolerance limit (50 ng/kg) accepted by the European Union. It was therefore concluded that, high occurrence of AFM1 in yoghurt is a serious risk for public health.

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Detection of aflatoxin M1 in cheese samples by ELISA

Cited by 99 publications

References 15 publications

Determination of Various Mycotoxin Concentrations in the Feedstuffs and Feed Produced by A Feed Manufacturer in Turkey

Determination of Various Mycotoxin Concentrations in the Feedstuffs and Feed Produced by A Feed Manufacturer in Turkey

Aflatoxin Levels in Roughage, Concentrates, Compound Feed and Milk Samples from Dairy Farms in Erzurum Province

Aflatoxin M1 Contamination in Traditional Yoghurts Produced in Guilan Province, Iran

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