Detection of aflatoxin M1 in powdered milk and sweetened condensed milk products in several cities in Java with HPLC-fluorescence method

Wijaya, Hanny; Wardayanie, Ning Ima Arie; Widjajanti, Rochmi; Silitonga, Rhoito Frista

doi:10.1088/1755-1315/102/1/012028

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…However, only six samples of branded milk products were analyzed in this study that is not sufficient to give general conclusion. High frequent of AFM1 contamination in branded milk products was also reported by Wijaya et al (2017) who found AFM1 contaminations in 85% of powdered milk samples and 68% of sweetened condensed milk samples that were collected in several cities in Java. Their survey indicated 55% of powdered milk samples contain AFM1 between 50-200 ng/kg.…”

Section: Discussionsupporting

confidence: 67%

Estimation of Aflatoxin M1 Exposure through Consumption of Various Dairy Milk Products in Yogyakarta, Indonesia (ESTIMASI PAPARAN AFLATOKSIN M1 MELALUI KONSUMSI BERBAGAI PRODUK SUSU DI YOGYAKARTA, INDONESIA)

Sumantri

Purwanti

Nuryono

et al. 2019

JVet

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This study was conducted to investigate the occurrence of aflatoxin M1 (AFM1) in various milk products marketed in Yogyakarta Province (Indonesia) and to estimate the exposure of aflatoxin through contaminated milk consumption. Fresh milk (n=20), pasteurized milk (n=16), and recombined milk products (n=6) were sampled for AFM1 concentration testing by a competitive ELISA test using ELISA kit for AFM1 assay. A survey was conducted to interview consumers (n=88) on milk consumption habit (milk type and amount of consumption). ELISA assays showed 92.5% of samples werecontaminated with AFM1 in a range of 24-570 ng/L (average: 216 ng/L). The highest average AFM1 concentration was detected in pasteurized milk sample (244 ng/L), followed by fresh milk (219 ng/L), and the lowest was in recombined milk sample (131 ng/L). However, 100% of recombined milk samples had AFM1 concentration >50-500 ng/L. Thus, recombined milk product was most likely the main source of AFM1 intake due to its high daily consumption in all age groups. Based on AFM1 levels found in milk and consumption of corresponding milk sample, it was estimated that the overall AFM1 exposure ranges from 1.23 ng/kg body weight/day (in 6-15 year-old children) up to 5.26 ng/kg body weight/day (in 3-5 year-old children). In conclusion, this study revealed high occurrences of AFM1 dairy milk marketed in Yogyakarta. Although levels of AFM1 contamination were in Indonesian regulatory limit, high exposure of aflatoxin found in all age groups of consumer. Thus, this preliminary study provides evidence that AFM1 contaminated milk is a serious public health hazard in Indonesia.

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Section: Discussionsupporting

confidence: 67%

Estimation of Aflatoxin M1 Exposure through Consumption of Various Dairy Milk Products in Yogyakarta, Indonesia (ESTIMASI PAPARAN AFLATOKSIN M1 MELALUI KONSUMSI BERBAGAI PRODUK SUSU DI YOGYAKARTA, INDONESIA)

Sumantri

Purwanti

Nuryono

et al. 2019

JVet

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“…Wijaya et al (7) detected AFM1 in powdered and sweetened condensed milk available in several cities in Java. The study reported that the concentration of AFM1 in powdered milk ranged from undetectable to 0.549μg/kg, with 55% of the contaminations ranged between lest than 0.05 to 0.2μg/kg.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the AFM1 levels in sweetened condensed milk ranged from undetectable to 0.056 μg/kg, with 43.7% of the contaminations ranged between 0.025 and 0.05μg/kg. All of the powdered and sweetened condensed milk samples met the maximum allowable level of AFM1, according to Indonesian standards (7). Nor Shifa Shuib et al determined the AFM1 concentration in milk and dairy products using HPLC fluorescence with postcolumn photochemical derivatization.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the presence of AFM1 in milk and dairy products is a major risk to humans (6). AFM1 can be found in dairy products because it is resistant to heat during processing (7). AFM1 is quite stable during normal milk processing, such as pasteurization, and if present in raw milk, it may survive and appear in the final products available for human consumption.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Aflatoxin M1 in Pasteurized Liquid and Powdered Milk Products Imported to Iran

Maymand¹,

Mazaheri²,

Mehrdar

2019

IJT

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Background: Mycotoxins are the secondary metabolites of molds and have adverse effects on humans, animals, and crops, resulting in illnesses and economic losses. Aflatoxin M1 (AFM1) is a hepatocarcinogen found in the milk from animals that have consumed feeds contaminated with aflatoxin B1 (AFB1). Milk is a highly nutritious food and is a source of necessary macro- and micro-nutrients for the growth, development and maintenance of human health. Methods: The presence of AFM1 was investigated in 70 samples of imported pasteurized and powdered milk products available to the Iranian consumers. The level of AFM1 was determined by HPLC method equipped with immunoaffinity cleanup. Results: The results showed that 32% of the analyzed samples were positive for AFM1 at 0.05-3.31 μg/kg. Also, 16% of analyzed samples were positive for AFM1at concentrations higher than the limit permitted by the Iranian standards. Conclusion: The detection of AFM1 contamination in the analyzed samples indicates the importance of the health of animal feeds. Thus, monitoring the imported feed materials, especially those arriving at Iranian borders is crucial in the prevention of AFM1 and AFB1 contaminations spreading across the domestic market.

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“…Fillaeli (2007) observed 95% of milk samples from Yogyakarta were contaminated with AFM1 at concentration level of 33 to 113 ng/L, Nuryono et al (2009) reported 70% of the samples observed contaminated with AFM1 at concentration level ≤10 ng/L and Nurhayati 2014found 54 out of 57 pasteurised milk were consisted of AFM1 at concentration level of 20.77 to 458.87 ng/L. Aflatoxin M1 also was analysed on 20 powdered milk collected from Serang, Bandung, Semarang and Surabaya, and had been reported in the range from undetectable to 0.549 µg/kg and the highest data (55%) was distributed in concentration range of >0.05 µg/kg to 0.2 µg/kg (Wijaya et al 2018).…”

Section: Contamination Of Afm1 In Raw Milk Samplesmentioning

confidence: 99%

Aflatoxin M1 in fresh dairy milk from small individual farms in Indonesia

Widiastuti¹,

Anastasia²

2018

JITV

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<p>This present study was aimed to investigate the presence of aflatoxin M1 (AFM1) residue in fresh dairy milk collected from small dairyl farms. A total of 104 samples of fresh cow's milk were collected in Pengalengan-Bandung and Sukabumi (West Java province), and Tanggamus (Lampung province) in April and September 2012. All samples were analyzed by a high performance liquid chromatography and detected with fluorescence detector after extraction with organic solvents. Contamination of AFM1 was found on 1.96% (1/51) from the samples collected in April 2012 at concentration of 1.20 ng/L and 39.63% (21/53) from the samples collected in September 2012 at concentration of 1.20 ng/L 1.0 – 34.1 ng/L. Those positive samples were obtained from Pangalengan and Sukabumi, but none for those samples collected from Tanggamus both on collection time April nor September 2012. In those positive samples for AFM1, there is no sample contained AFM1 above the maximum level (ML) regulated in Indonesia (500 ng/L or 0.5 µg/L). Low contamination levels of AFB1 in the range of 0.38 to 6.64 µg/kg found in supplemental feed samples from the same sampling time and locations. The findings of AFM1 contamination in raw fresh milk from this study caused no harm to the consumers. However, regular monitoring on the presence of AFM1 in dairy milk and aflatoxin B1 (AFB1) in dairy cattle feed is necessary to ensure the protection of human health.</p>

show abstract

Detection of aflatoxin M1 in powdered milk and sweetened condensed milk products in several cities in Java with HPLC-fluorescence method

Cited by 5 publications

References 7 publications

Estimation of Aflatoxin M1 Exposure through Consumption of Various Dairy Milk Products in Yogyakarta, Indonesia (ESTIMASI PAPARAN AFLATOKSIN M1 MELALUI KONSUMSI BERBAGAI PRODUK SUSU DI YOGYAKARTA, INDONESIA)

Estimation of Aflatoxin M1 Exposure through Consumption of Various Dairy Milk Products in Yogyakarta, Indonesia (ESTIMASI PAPARAN AFLATOKSIN M1 MELALUI KONSUMSI BERBAGAI PRODUK SUSU DI YOGYAKARTA, INDONESIA)

Determination of Aflatoxin M1 in Pasteurized Liquid and Powdered Milk Products Imported to Iran

Aflatoxin M1 in fresh dairy milk from small individual farms in Indonesia

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