Detection of Aleutian Disease Virus DNA in Tissues of Naturally Infected Mink

Haas, Ludwig; Löchelt, Martin; Kaaden, Oskar‐Rüger

doi:10.1099/0022-1317-69-3-705

Cited by 17 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During productive infection in mink (Alexandersen & Bloom, 1987;Haas et al, 1988) or cell cultures (Bloom et al, 1983;L6chelt & Kaaden, 1987) ds replicative form (RF) DNA and ss virion DNA are detectable in cellular lysates. Due to the configuration of the terminal hairpins in either the extended (palindrome) or foldback (hairpin), forms, the ADV monomer (M) RF DNA displays a length heterogeneity of about 200 bp.…”

Section: Introductionmentioning

confidence: 99%

A Novel Replicative Form DNA of Aleutian Disease Virus: the Covalently Closed Linear DNA of the Parvoviruses

Löchelt

Delius²,

Kaaden³

1989

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

SUMMARYThe analysis of replicative form (RF) DNA of Aleutian disease virus (ADV) by alkaline gel electrophoresis revealed that all RF DNA species segregate into DNA single strands which represent integral multiples of a genome equivalent. This" demonstrates that as with other autonomous parvoviruses, the virion and complementary DNA strands are frequently linked by hairpin structures and that also, nicks are present at subterminal sites. Approximately 50 ~ of the Y-terminal hairpins contain a subterminal nick whereas no nick is detectable in the Y-terminal hairpin. This finding together with the presence of nicks in the 3' palindrome sequence of the dimer RF DNA (D RF DNA) bridge fragment is the first experimental proof for the so far hypothetical substrate specificity of a nickase. A novel DNA structure was identified in the monomer (M) RF DNA population. This molecule, designated 'monomer covalently closed linear RF DNA' (Mccl RF DNA), consists of a continuous, selfcomplementary, circular polynucleotide chain of twice the genome length. It was directly visualized by electron microscopy that denatured ADV M RF DNA is a singlestranded circular molecule of twice the genome length with covalently closed terminal hairpins on either end. Alkaline gradient centrifugations, enzymic assays and electrophoretic techniques confirmed the proposed structure. Moreover, evidence was obtained that the D RF DNA species contains an analogous Dccl RF DNA. It is suggested that the newly described Mccl RF DNA form is an important intermediate common to the DNA replication of all autonomously replicating parvoviruses.

show abstract

Section: Introductionmentioning

confidence: 99%

A Novel Replicative Form DNA of Aleutian Disease Virus: the Covalently Closed Linear DNA of the Parvoviruses

Löchelt

Delius²,

Kaaden³

1989

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The most consistent histological finding is periportal lymphocytic infiltrates (Fig. This technique can also be used on formalin-fixed, paraffin-embedded biopsy samples (Haas et al, 1988). Bile duct hyperplasia and periportal fibrosis have also been reported.…”

Section: Treatment and Controlmentioning

confidence: 89%

Biology and Diseases of Ferrets

Mayer

Marini

Fox

2015

Laboratory Animal Medicine

View full text Add to dashboard Cite

“…7) known to contain ADV RF DNA (Haas et al, 1988). In organs with low amounts of RF DNA, such as spleens and the lymph nodes (Haas et al, 1988), Mccl RF DNA could not be demonstrated.…”

Section: Detection Of MCCL Rf Dna In Bone Marrow Samples Of Naturallymentioning

confidence: 99%

“…The targets in vivo are lymphoid cells in mesenteric and peripheral lymph nodes, spleen, bone marrow and peripheral blood (Bloom et al, 1985;Roth et al, 1984;Kaaden et al, 1986;Haas et al, 1988) and alveolar type II cells in mink kits infected neonatally (Alexandersen & Bloom, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…The isolation of ADV RF DNA from infected cell cultures (L6chelt & Kaaden, 1987) and organs of naturally infected mink (Haas et al, 1988) and the release of viral ssDNA from infectious virions (L6chelt & Kaaden, 1987) have been described. DNA electrophoresis on neutral or alkaline agarose gels was performed as published (L6chelt et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The monomer covalently closed linear replicative form DNA is an intermediate of Aleutian disease parvovirus DNA replication

Löchelt¹,

Haas²,

Kaaden³

1990

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

In this report we present data indicating that the recently detected monomer covalently closed linear replicative form DNA (Mccl RF DNA) is an intermediate of Aleutian disease virus (ADV) DNA replication. This DNA molecule is characterized by covalently closed terminal hairpins on either end of the linear ds genomic molecule. Its first detection early after infection in vitro, the association with ADV-specific replication complexes and the de novo synthesis of Mccl RF DNA by isolated replication complexes point to an important role in parvoviral DNA replication. The presence of the Mccl RF DNA in extracts of virusproducing bone marrow cells of naturally infected mink rules out the possibility that this DNA form represents a mere cell culture artefact. The detection of the ADV non-structural protein 1 (NS1) in replication complexes supports the view that this protein is involved in ADV DNA replication.

show abstract

Detection of Aleutian Disease Virus DNA in Tissues of Naturally Infected Mink

Cited by 17 publications

References 22 publications

A Novel Replicative Form DNA of Aleutian Disease Virus: the Covalently Closed Linear DNA of the Parvoviruses

A Novel Replicative Form DNA of Aleutian Disease Virus: the Covalently Closed Linear DNA of the Parvoviruses

Biology and Diseases of Ferrets

The monomer covalently closed linear replicative form DNA is an intermediate of Aleutian disease parvovirus DNA replication

Contact Info

Product

Resources

About