Detection of Aleutian disease virus by loop-mediated isothermal amplification

Zhang, Zhuo; Wang, Bin; Hu, Shouping; Zhang, Jiaoer; He, Xijun; Zheng, Shangen

doi:10.1007/s13337-015-0265-9

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Therefore, this method has been suggested to be a more reliable and specific approach to detect AMDV in mink [118]. In addition, loopmediated isothermal amplification (LAMP) has also been designed to recognize different regions of the VP2 gene of AMDV, and it was shown to outperform PCR test of AMDV positive mink, making it a rapid and reliable approach [119]. Taken together, the use of novel technologies to detect AMDV-positive mink could provide reinforcing tools to help limit viral spread.…”

Section: Amdv Preventative Measuresmentioning

confidence: 99%

AMDV Vaccine: Challenges and Perspectives

Markarian

Abrahamyan

2021

Preprint

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Aleutian mink disease virus (AMDV) is known to cause the most significant disease in the mink industry. It is globally widespread and manifested as a deadly plasmacytosis and hyperglobulinemia. So far, measures to control viral spread have been limited to manual serological testing for AMDV-positive mink. Further, due to the persistent nature of this virus, attempts to eradicate Aleutian disease (AD) have largely failed. Therefore, effective strategies to control viral spread are of crucial importance for wildlife protection. One potentially key tool in the fight against this disease is by immunization of mink against AMDV. Throughout many years, several researchers have tried to develop AMDV vaccines and demonstrated varying degrees of protection in mink by those vaccines. Despite these attempts, there are currently no vaccines available against AMDV, allowing the continuation of the spread of Aleutian disease. Herein, we summarize previous AMDV immunization attempts in mink as well as other preventative measures with the purpose to shed light on future studies designing such a potentially crucial preventative tool against Aleutian disease.

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Section: Amdv Preventative Measuresmentioning

confidence: 99%

AMDV Vaccine: Challenges and Perspectives

Markarian

Abrahamyan

2021

Preprint

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“…In the detection of foodborne pathogens, molecular biological detection technology as a novel identification method has been widely used, which mainly comprises nucleic acid probe techniques, PCR, real-time PCR, and loop-mediated isothermal amplification (LAMP; Wang et al, 2015;Zhang et al, 2015;Tomas et al, 2018;Liang et al, 2019). Among these, the LAMP platform is relatively simple, rapid, and sensitive, which demonstrates greater promise for practical applications in the field (Dhama et al, 2014;Wu et al, 2014;Sarowska et al, 2016;Fu et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

Screening of specific nucleic acid targets for Cronobacter sakazakii and visual detection by loop-mediated isothermal amplification and lateral flow dipstick method in powdered infant formula

Xue

Wang

et al. 2021

Journal of Dairy Science

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Due to the lack of specific genes for rapid detection methods of Cronobacter sakazakii in food samples, whole genome sequence analysis was performed in this investigation using the basic local alignment search tool. Forty-two DNA fragments unique to C. sakazakii were mined, then primers were designed and screened by PCR and loop-mediated isothermal amplification (LAMP). Two primer sets, CS1 and CS31, were found as specific and stable primers, with their corresponding nucleic acid targets the CSK29544_00235 gene and CSK29544_03484 gene, respectively. Furthermore, compared with 3 genes reported previously, these 2 genes were verified as more specific to C. sakazakii among Cronobacter species, by sequence similarity alignment using Cronobacter MLST databases (http: / / pubmlst .org/ cronobacter). The specificity of the LAMP reaction approached 100% by using 48 bacterial strains, which included 22 C. sakazakii strains. Subsequently, LAMP was combined with visual lateral flow dipstick (LFD) based on the above 2 nucleic acid targets, and was demonstrated as a rapid, efficient method with high specificity. Finally, the detection sensitivity of this assay system for pure cultures and artificially contaminated milk was measured as 4.5 × 10 0 cfu/mL and 5.7 × 10 1 cfu/g, respectively. Total time to detection for this assay was within 2 h. Thus, the establishment of this LAMP-LFD method shows great significance and potential for rapid detection of C. sakazakii in powdered infant formula.

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Detection of Aleutian disease virus by loop-mediated isothermal amplification

Cited by 2 publications

References 16 publications

AMDV Vaccine: Challenges and Perspectives

AMDV Vaccine: Challenges and Perspectives

Screening of specific nucleic acid targets for Cronobacter sakazakii and visual detection by loop-mediated isothermal amplification and lateral flow dipstick method in powdered infant formula

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