Detection of amyloid β protein in the urine of Alzheimer's disease patients and healthy individuals

Takata, Makoto; Nakashima, Mitsuyoshi; Takehara, Taro; Baba, Hideyo; Machida, Kazuyuki; Akitake, Yoshiharu; Ono, Kazuhiko; Hosokawa, Masato; Takahashi, Mitsuo

doi:10.1016/j.neulet.2008.02.019

Cited by 26 publications

(21 citation statements)

References 35 publications

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“…The biomarkers that have been strongly associated with the diagnosis of Alzheimer's are Aβ-40, Aβ-42 and tau proteins [30][31][32][33][34][35]. All these biomarkers have been isolated from the cerebrospinal fluid and provide a fair estimation of Alzheimer's disease.…”

Section: Review Of Literaturementioning

confidence: 99%

“…Hence, the focus for diagnosis of Alzheimer's has been shifted towards less invasive procedures. Assay of blood-based biomarkers has received great attention for the diagnosis and detection of Alzheimer's [30][31][32]. Studies have indicated that the plasma concentrations of Aβ-40 and Aβ-42 are high in individuals suffering from Alzheimer's.…”

Section: Review Of Literaturementioning

confidence: 99%

“…Other blood-based biomarkers have also been implicated in Alzheimer's diseases. These biomarkers include conformational alterations in p53 protein, IL-1 receptor antagonist, C-reactive protein, alpha-1 chymotrypsin and tumor necrosis factor-alpha [31,32]. Studies have suggested that the etiology Alzheimer's is extremely complex and diagnosis should not be based on any one specific biomarker [30][31][32][33][34][35].…”

Section: Review Of Literaturementioning

confidence: 99%

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Evaluation of Different Factors Leading to the Genesis of Alzheimer’s Disease: Research Proposal on Dementia

Pei¹

2017

Epidemiology (Sunnyvale)

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Alzheimer's disease is a neurodegenerative disorder that is featured by the momentary loss of memory and forgetfulness. Alzheimer's disease and dementia affect millions of individuals all over the globe. Individuals suffering from Alzheimer's or dementia are prone to accidental falls and are at increased risk of life-threatening injuries. Identification of the predisposing risk factors would help to reduce the prevalence and complications of both these diseases in the near future. The present article portrayed a research proposal for evaluating the role of probable factors leading to the genesis of Alzheimer's disease. The present study would be conducted as a cohort and crosssectional study. The study would be based on quantitative analysis and end-points would be reflected through quantitative data. The study would be conducted on 5000 participants belonging to 4 cities. The cities selected for the present study would be Shanghai, Beijing, Guangzhou, and Wuhan. MMSE would be used to classify different grades of dementia. However, it could not be used to portray the presence of Alzheimer's disease amongst the study participants. Therefore, plasma Aβ-42/Aβ-40 ratio and plasma TNF-alpha/IL-1β-40 ratio would be implemented for confirming the presence of Alzheimer's disease. Regression analysis would be conducted for dementia and Alzheimer's with the individual risk factors. Based on the evaluation of risk factors, a public health model would be planned and implemented. The public health model would involve the family members of affected individuals. Implementation of a public health model is speculated to reduce the progression of Alzheimer's or dementia.

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Section: Review Of Literaturementioning

confidence: 99%

Section: Review Of Literaturementioning

confidence: 99%

Section: Review Of Literaturementioning

confidence: 99%

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Evaluation of Different Factors Leading to the Genesis of Alzheimer’s Disease: Research Proposal on Dementia

Pei¹

2017

Epidemiology (Sunnyvale)

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“…Amyloid β peptide is a risk factor for AD and can be found in the urine of AD patients. When analyzed by Western blotting, 0.003 to 1.11 ng in 1 ml of amyloid β peptide was obtained from a urine sample [55]. (Table 1) Summarized the other biomarkers of AD and their sample sources [56-65].…”

Section: Fish-based Detection Of Chromosomal Abnormalitiesmentioning

confidence: 99%

Fish-on-a-chip: a sensitive detection microfluidic system for alzheimer's disease

Devadhasan

Kim

2011

J Biomed Sci

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Microfluidics has become an important tool in diagnosing many diseases, including neurological and genetic disorders. Alzheimer's disease (AD) is a neurodegenerative disease that irreversibly and progressively destroys memory, language ability, and thinking skills. Commonly, detection of AD is expensive and complex. Fluorescence in situ hybridization (FISH)-based microfluidic chip platform is capable of diagnosing AD at an early stage and they are effective tools for the diagnosis with low cost, high speed, and high sensitivity. In this review, we tried to provide basic information on the diagnosis of AD via FISH-based microfluidics. Different sample preparations using a microfluidic chip for diagnosis of AD are highlighted. Moreover, rapid innovations in nanotechnology for diagnosis are explained. This review will provide information on dynamic quantification methods for the diagnosis and treatment of AD. The knowledge provided in this review will help develop new integration diagnostic techniques based on FISH and microfluidics.

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“…It will be more attractive and practical for β-amyloid 42 to be detected in other physiological fluid including plasma, serum, and others. Consequently, research efforts have been devoted to detecting β-amyloid 42 in physiological fluid other than CSF [17][18][19][20][21]. Therefore, it is desirable to have a simple-use detection method for β-amyloid 42 in a physiological fluid other than CSF, such as serum and others.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-effective Thin Gold Film Biosensor

Dai¹,

Molazemhosseini²,

Liu³

2017

Preprint

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A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffer saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The biorecognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K 4 Fe (CN) 6 and K 3 Fe (CN) 6 . Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction. The biosensor was a three-electrode electrochemical system, and the working and counter electrodes were 50 nm thin gold film deposited by sputtering technique. The reference electrode was a thick-film printed Ag/AgCl electrode. Laser ablation technique was used to define the size and structure of the biosensor. Cost-effective roll-to-roll manufacturing process was employed in the fabrication of the biosensor making it simple and relatively inexpensive. Self-assembled monolayers (SAM) of 3-Mercaptopropionic acid (MPA) was employed to covalently immobilize the thiol group on the gold working electrode. A carbodiimide conjugation approach using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was undertaken for cross-linking antibody of β-amyloid 42 to the carboxylic groups on one end of the MPA. The antibody concentration of β-amyloid 42 used was 18.75µg/mL. The concentration range of β-amyloid 42 in this study was from 0.0675µg/mL to 0.5µg/mL for both PBS and undiluted human serum. DPV measurements showed excellent response in this antigen concentration range. Interference study of this biosensor was carried out in the presence of Tau protein antigen. Excellent specificity of this β-amyloid 42 biosensor was demonstrated without interference by other species such as T-tau protein.Keywords β-amyloid 42; differential pulse voltammetry; Neuro-degenerative disorders; ferrocyanide/ferricyanide redox couple Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Detection of amyloid β protein in the urine of Alzheimer's disease patients and healthy individuals

Cited by 26 publications

References 35 publications

Evaluation of Different Factors Leading to the Genesis of Alzheimer’s Disease: Research Proposal on Dementia

Evaluation of Different Factors Leading to the Genesis of Alzheimer’s Disease: Research Proposal on Dementia

Fish-on-a-chip: a sensitive detection microfluidic system for alzheimer's disease

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-effective Thin Gold Film Biosensor

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