Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

Mongruel, Anna Cláudia Baumel; Benevenute, Jyan Lucas; Ikeda, Priscila; André, Marcos Rogério; Machado, Rosangela Zacarias; Carrasco, Adriano de Oliveira Torres; Seki, Meire Christina

doi:10.1590/s1984-29612017042

Cited by 9 publications

(7 citation statements)

References 51 publications

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“…(1.67%; 7/418). Noteworthy, even though sequences similar to A. phagocytophilum have been detected in several wild animals from Brazil, negative results have been reported when a qPCR specific for this agent and based on msp-2 gene was used [6,55]. Together, these findings suggest that a putative new Anaplasma species is circulating among wild animals from Brazil.…”

Section: Discussionmentioning

confidence: 96%

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

et al. 2021

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The Anaplasmataceae family (order Rickettsiales) encompasses obligately intracellular bacteria of the genera Anaplasma, Ehrlichia, and Neorickettsia. Together with Coxiella burnetii (Coxiellaceae family, order Legionellales), these bacteria represent important causative agents of diseases in humans and animals. The scarcity of studies that investigated the occurrence of these agents in bats and their associated ectoparasites, emphasizes the need to achieve a better understanding of the role of these animals in the maintenance of such bacteria. Herein, 418 samples (133 blood, 135 spleen, and 150 ectoparasites) are collected from 135 non-hematophagous bats belonging to 12 species in a periurban area of Campo Grande city, Mato Grosso do Sul state, midwestern Brazil. In the results, 1.65% (7/418), 12.04% (50/418), and 13.63% (57/418) of samples are positive in PCR assays for Anaplasma spp. (16S rRNA gene), Ehrlichia spp. (dsb gene), and Neorickettsia spp. (16S rRNA gene), respectively. Anaplasma spp. and Neorickettsia spp. are detected in one (5.26%) Ornithodoros hasei tick larva. Ehrlichia spp. is detected in 14% of bat flies (represented by Megistopoda aranea, Trichobius costalimai, and Strebla hertigi), 6% of tick larvae (O. hasei), 12% of Spinturnicidae mites (represented by Periglischrus sp., P. torrealbai, and P. acutisternus), and 38% of Macronyssidae mites (Steatonyssuss sp.). The obtained sequences are observed to be similar to Anaplasma phagocytophilum (97.42–97.6% identified), Ehrlichia minasensis (96.73–100% identified), Neorickettsia risticii (96.7–100% identified), and Neorickettsia findlayensis (95.07–100% identified) by BLASTn analyses, and closely related to Ehrlichia ruminantium by phylogenetic analyses based on the gltA gene. No bat samples (blood/spleen) are positive in the qPCR assay for C. burnetii based on the IS1111 gene. The present work shows, for the first time, the occurrence of Anaplasmataceae in bats and associated ectoparasites (ticks, mites, and bat flies) from Brazil.

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Section: Discussionmentioning

confidence: 96%

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

et al. 2021

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“…Several Anaplasma strains have been detected using the small-subunit rRNA (16S rRNA) which has proven to be a sensitive molecular tool to confirm the presence of these pathogens' DNA in the investigated ticks and/or animal hosts. However, 16S rRNA gene is highly conserved with few polymorphic positions, therefore, closely related species and strains cannot be distinguished (Mongruel et al 2017). On the other hand, it has been reported that the major surface protein 4 (msp 4)-that is encoded by msp4 gene-is associated with faster evolution than other nuclear genes and involved in interactions with host cells (Yang et al 2015).…”

Section: Impactsmentioning

confidence: 99%

“…However, 16S rRNA gene is highly conserved with few polymorphic positions, therefore, closely related species and strains cannot be distinguished (Mongruel et al . ). On the other hand, it has been reported that the major surface protein 4 (msp 4)‐that is encoded by msp4 gene‐is associated with faster evolution than other nuclear genes and involved in interactions with host cells (Yang et al .…”

Section: Introductionmentioning

confidence: 97%

Molecular characterization of Anaplasma and Ehrlichia in ixodid ticks and reservoir hosts from Palestine: a pilot survey

Zaid

Ereqat

Nasereddin

et al. 2019

Veterinary Medicine & Sci

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Tick‐borne anaplasmosis and ehrlichiosis are clinically important emerging zoonoses usually overlooked by veterinarians and physicians alike. This study aimed at detecting and genetically characterizing Ehrlichia and Anaplasma species in ixodid ticks and their animal hosts from the West Bank, Palestine. A total of 723 ixodid ticks belonging to three genera ( Rhipicephalus, Hyalomma, Haemaphysalis ) were collected from dogs, sheep, goats and camels. In addition, 189 blood samples were collected from dogs, sheep, camels, horses and a goat from the West Bank, Palestine. All tick and blood samples were investigated for the presence of Anaplasma and Ehrlichia targeting a 345 bp fragment of the 16S rRNA gene followed by sequence analysis. The infection rate of Anaplasma spp. in ticks was 6.5% (47/723). Anaplasma platys was identified in 28% (13/47) of them. Whereas, based on a partial sequence (851 bp) of msp4 gene, 38% (18/47) were identified as A. ovis . The species of the remaining 16 positive samples (16/47, 34%) could not be identified. Simultaneously, the infection rate of Ehrlichia spp. in the ticks was 0.6% (4/723). Three of which were E. canis and one was Ehrlichia spp. The infection rate of A. platys in dogs’ blood samples was 10% (13/135), while it was 1.5% (2/135) for E. canis . The infection rate of Anaplasma in sheep blood samples was 40% (19/47), out of which 26% (5/19) were caused by A. ovis as revealed by msp4 ‐ PCR . Implementation of purely‐spatial analysis by sa TS can for all cases of Anaplasma revealed two statistically significant clusters in two districts; Tubas town and Majdal‐Bani‐Fadil village on the western hills of the Jordan Valley. Most cases of Anaplasma (83%) were from rural areas where life cycle components (vector, host and reservoir) abundantly interact. This study is the first in Palestine to reveal the presence of Anaplasma and Ehrlichia in ticks, dogs and sheep providing crucial platform for future epidemiological surveys and control strategies in the country and region.

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“…Anaplasma phagocytophilum has a wide host range and complex reservoir hosts especially in rural and pastoral communities which have a shared ecosystem between humans, livestock, and wildlife [5,6]. In addition to the A. phagocytophilum livestock-human cycle, there is evidence of the bacterium circulating in wildlife vertebrates and reports show that small mammals such as mice, wood rats, and birds can harbor A. phagocytophilum without manifesting clinical signs [7][8][9][10]. Tese small mammals are reservoir hosts for many tick-borne pathogens as well as feeding hosts for various stages of tick vectors [11].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Anaplasma phagocytophilum in Small Mammals and Infesting Ticks in Laikipia County, Kenya

Mosha,

Kuria,

Otiende

et al. 2024

Veterinary Medicine International

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Anaplasmosis is a set of disease conditions of various mammals caused by bacteria species of the genus Anaplasma. These are sub-microscopic, Gram-negative, obligate intracellular pathogens that infect both vertebrate and invertebrate hosts. Significant species that infect domestic and wildlife animals include Anaplasma marginale, Anaplasma ovis, Anaplasma mesaeterum, Anaplasma platys, and Anaplasma phagocytophilum. Although A. phagocytophilum has a widespread distribution, there are only a few epidemiological reports from sub-Saharan Africa. This study focused on molecular detection and characterization of A. phagocytophilum in small mammals and their infesting ticks in Laikipia County, Kenya. A total of 385 blood and 84 tick archival samples from small mammals (155 females and 230 males) were analyzed. The blood samples were subjected to a nested PCR-HRM melt analysis using species-specific primers to amplify the 16S ribosomal RNA genes. The ticks were also subjected to nested PCR-HRM involving 16S rRNA gene primers. Anaplasma phagocytophilum DNA was detected in 19 out of 385 samples using species-specific 16S rRNA gene primers giving a prevalence of 4.9% for A. phagocytophilum. Analysis of the tick’s samples using 16S rRNA gene species-specific primers also detected A. phagocytophilum in 3 samples from Haemaphysalis leachi ticks (3/84) equivalent to prevalence of 3.6%. Sequencing of 16S rRNA PCR products confirmed A. phagocytophilum in small mammals and ticks’ samples. Phylogenetic analysis of the haplotype from this study demonstrated a close ancestral link with strains from Canis lupus familiaris, Alces alces, Apodemus agrarius, and ticks (Haemaphysalis longicornis) reported in Europe, China, and Africa. Comparison was also made with a known pathogenic A. phagocytophilum variant HA and a nonpathogenic variant 1 that were clustered into a distinctive clade different form haplotypes detected in this study. All the haplotype sequences for A. phagocytophilum from this study were submitted and registered in GenBank under the accession numbers OQ308965–OQ308976. Our study shows that small mammals and their associated ticks harbor A. phagocytophilum. The vector competence for H. leachi in A. phagocytophilum transmission should further be investigated.

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Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

Cited by 9 publications

References 51 publications

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

Molecular characterization of Anaplasma and Ehrlichia in ixodid ticks and reservoir hosts from Palestine: a pilot survey

Molecular Detection of Anaplasma phagocytophilum in Small Mammals and Infesting Ticks in Laikipia County, Kenya

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