Detection of Anti-CandidaalbcansIgE Antibodies in Vaginal Washes from Patients with Acute Vulvovaginal Candidiasis

Regúlez, P; Fernández, J.; Moragues, María Dolores; Schneider, J.; Quindós, G.; Pontón, José

doi:10.1159/000292536

Cited by 25 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…30 One study has described a higher level of non- C. albicans species occurrence over time. 31 As stated by Ferrazza et al 32 , almost 25% of VVC cases were caused by non- albicans species. Comparing the results of our study with those other studies which demonstrated, C. glabrata to be the second most significant species among VVC cases because of its higher frequency and increased rate of clinical resistance to antifungals.…”

Section: Discussionmentioning

confidence: 91%

Antifungal susceptibility testing of vulvovaginal Candida species among women attending antenatal clinic in tertiary care hospitals of Peshawar

Khan

Ahmed

Gul

et al. 2018

IDR

View full text Add to dashboard Cite

BackgroundVulvovaginal candidiasis (VVC) is considered as a pervasive gynecological problem among women worldwide. Owing to this fact, in the current study, we aimed at assessing the prevalence rate of Candida spp. causing VVC in symptomatic pregnant women and their antifungal susceptibility pattern.MethodsThis study was carried out in the tertiary care hospitals of Peshawar during the period of July 1, 2016 to December 31, 2016. The study group included 450 pregnant women in the age group of 17–44 years with symptoms of excessive vaginal discharge, pain and pruritis. In all, 108 pregnant women were culture positive for Candida. Antimicrobial susceptibility testing (AST) was conducted on specimens against various azoles and polyene F group of antifungals.ResultsOut of 108 Candida spp. isolated from vaginal swabs, there were 45 (41.7%) Candida albicans, 18 (16.7%) Candida tropicalis, 18 (16.7%) Candida krusei, 16 (14.8%) Candida glabrata and 11 (10.2%) Candida dubliniensis. According to age distribution, 27 years was the mean age. Pregnancy trimester distribution among patients was as follows: 21 (19.4%) patients were in their first trimester, 65 (60.2%) patients were in their second trimester and 22 (20.4%) patients were in the third trimester. Susceptibility of fluconazole was determined as follows: 33.3% of the Candida isolates were sensitive, 4.6% were susceptible dose dependent (SDD) and 62% were resistant. Susceptibility of Candida spp. with respect to nystatin in patients with VVC was as follows: 25% were sensitive, 16.7% were SDD and 58.3% were resistant. Susceptibility of clotrimazole was analyzed, and it was sensitive in 21.3% of patients, SDD in 19.4% of patients and resistant in 59.3% of patients. Voriconazole susceptibility was recorded to be sensitive in 85.2% of patients, SDD in 4.6% of patients and resistant in 10.2% of patients suffering from VVC. Susceptibility results for itraconazole in patients with VVC were as follows: 42.6% of patients were sensitive, 16.7% of patients were SDD, and 40.7% of patients were resistant.ConclusionIn this study, frequency of VVC was noted to be high in the second trimester of pregnancy, with the highest frequency of C. albicans isolated, followed by C. tropicalis and C. krusei. Antifungal susceptibility testing revealed that fluconazole was exceedingly resistant against Candida species (62%), followed by clotrimazole (59.3%) and nystatin (58.3%). On the contrary, voriconazole had the highest antimicrobial activity against Candida species (85.2%).

show abstract

Section: Discussionmentioning

confidence: 91%

Antifungal susceptibility testing of vulvovaginal Candida species among women attending antenatal clinic in tertiary care hospitals of Peshawar

Khan

Ahmed

Gul

et al. 2018

IDR

View full text Add to dashboard Cite

show abstract

“…It appears as white patches known as “plaques” which resemble milk curds. [13–15] One way to distinguish candida plaques from milk curds is that if the surface of the plaque is scraped away, a sore and reddened area will be seen underneath, which may sometimes bleed. It occurs most commonly in babies, particularly in the first few weeks of life.…”

Section: Types Of Fungal Infectionsmentioning

confidence: 99%

Emerging fungal infections among children: A review on its clinical manifestations, diagnosis, and prevention

Jain¹,

Jain²,

Rawat

2010

J Pharm Bioall Sci

View full text Add to dashboard Cite

The incidence of fungal infections is increasing at an alarming rate, presenting an enormous challenge to healthcare professionals. This increase is directly related to the growing population of immunocompromised individuals especially children resulting from changes in medical practice such as the use of intensive chemotherapy and immunosuppressive drugs. Although healthy children have strong natural immunity against fungal infections, then also fungal infection among children are increasing very fast. Virtually not all fungi are pathogenic and their infection is opportunistic. Fungi can occur in the form of yeast, mould, and dimorph. In children fungi can cause superficial infection, i.e., on skin, nails, and hair like oral thrush, candida diaper rash, tinea infections, etc., are various types of superficial fungal infections, subcutaneous fungal infection in tissues under the skin and lastly it causes systemic infection in deeper tissues. Most superficial and subcutaneous fungal infections are easily diagnosed and readily amenable to treatment. Opportunistic fungal infections are those that cause diseases exclusively in immunocompromised individuals, e.g., aspergillosis, zygomycosis, etc. Systemic infections can be life-threatening and are associated with high morbidity and mortality. Because diagnosis is difficult and the causative agent is often confirmed only at autopsy, the exact incidence of systemic infections is difficult to determine. The most frequently encountered pathogens are Candida albicans and Aspergillus spp. But other fungi such as non-albicans Candida spp. are increasingly important.

show abstract

“…Some studies have demonstrated the IgE presence in serum and vaginal secretions in 87% of women with vaginal candidiasis (Witikin et al, 1988;Regúlez et al, 1994) increasing the premise that the immunoglobulin role in the vaginal candidiasis pathogenesis is associated to the histamine released by locally mast cells. Furthermore, histamine is able to locally induce the prostaglandin E2 production that may be responsible for the local immune response and immunomodulation process (Witikin et al, 1988;Noverr et al, 2001;Regúlez et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Immune Response, Detection of IgE and PGE2 during Vaginal Candidiasis in Mice

Lucas¹,

Taglialegna²,

França³

et al. 2016

American Journal of Immunology

View full text Add to dashboard Cite

Abstract:Vulvovaginal candidiasis is an opportunistic infection that affects most women in adult life, but the defense mechanism remains to be elucidated. Animals treated with estradiol were inoculated with Candida albicans having high virulence power. The experimental control consisted of animal groups treated with estradiol and animals without treatment that were inoculated with physiologic serum. The vaginal wall was collected, at different times. The material was destined to the counting of Colony Forming Units (CFU), detection of PGE2, IgE and histological staining (hematoxylin and eosin, silver and toluidine blue) for the study of the infected vaginal section. Experimental infection was predominant due to hyphae and pseudohyphae parasitism, involving the keratinized layer of the vaginal stratified squamous epithelium, without compromise submucosal or muscular layer. Furthermore, it was observed mast and polymorphonuclear cells on vaginal tissue in response to the infection. On the other hand, IgE and PGE2 participated in the response to experimental C. albicans vaginal infection. The raise in these mediators matches with the load fungal increase during the infection evolution and with the presence of mast cell. These results suggest a probable atopic component involved in the vaginal candidiasis pathogenesis.

show abstract

Detection of Anti-CandidaalbcansIgE Antibodies in Vaginal Washes from Patients with Acute Vulvovaginal Candidiasis

Cited by 25 publications

References 16 publications

Antifungal susceptibility testing of vulvovaginal <em>Candida</em> species among women attending antenatal clinic in tertiary care hospitals of Peshawar

Antifungal susceptibility testing of vulvovaginal <em>Candida</em> species among women attending antenatal clinic in tertiary care hospitals of Peshawar

Emerging fungal infections among children: A review on its clinical manifestations, diagnosis, and prevention

Immune Response, Detection of IgE and PGE2 during Vaginal Candidiasis in Mice

Contact Info

Product

Resources

About