Detection of anti-topoisomerase I autoantibody in patients with silicosis

Abstract

Objectives: The aim of this study was to detect anti-topoisomerase l (anti-topo I) autoantibodies, which are known to be limited in systemic sclerosis patients, in silicosis patients with no clinical symptoms of autoimmune disease.Methods: Serum anti-topo I autoantibodies were detected using ELISA. Differences in clinical parameters between patients with and without anti-topo I autoantibodies were analyzed.Results: Seven of 69 patients had anti-topo I autoantibodies. These 7 patients showed elevated PaCO 2 values (P=0.0212), and inverse correlations between serum soluble Fas levels and PaCO 2 values were found.Conclusion: Anti-topo I autoantibodies were detected in 10.1% of silicosis patients without any clinical symptoms of autoimmune disease. The findings here suggest that the genesis of anti-topo l autoantibodies might be related to pulmonary involvement or lung fibrosis associated with progression of silicosis.

Immunostimulation by Silica Particles and the Development of Autoimmune Dysregulation

“…In addition, silicosis patients without bullous diseases tested positive for anti-desmoglein autoantibodies and the frequencies of HLA-DRB1*04, HLA-DQB1*03, *0303, and *05, and HLA-DPB1*0402 and *0501 alleles were higher in these patients than in a healthy Japanese control population in the literature [29,30]. Moreover, the relationship between the autoantibodies in silicosis patients and HLA phenotypes was also analyzed in silicosis patients with anti-topoisomerase I autoantibodies [30][31][32][33][34], and the results obtained revealed that the allelic frequency of HLA-DQB1*0402 was significantly higher in anti-topoisomerase I positive Japanese silicosis patients than in anti-topoisomerase I negative patients or healthy donors [30][31][32][33][34].…”

Environmental factors and human health: fibrous and particulate substance-induced immunological disorders and construction of a health-promoting living environment

“…2b) [45], a certain fraction of helper T cells in SIL is thought to survive longer with prolonged activation [45]. On the other hand, among the various auto-antibodies, we found in SIL serum [46][47][48][49][50], we detected a functional anti-Fas autoantibody (Fig. 2c) [50].…”

Detection, epitope‐mapping and function of anti‐Fas autoantibody in patients with silicosis

“…1,2 Since autoantibodies against unknown antigens are present in the serum of these patients, their sera have been analysed for autoantibodies directed against the Fas molecule, and here we report the detection of anti-Fas autoantibodies. In previous studies, we found autoantibodies directed against DNA topoisomerase I, 3 desmogleins 4 and caspase-8. 5 The SPOTs system is a technology that allows the solidphase synthesis of an extensive series of short peptides for the systematic analysis of antibody epitopes.…”

“…Since autoantibodies against unknown antigens are present in the serum of these patients, their sera have been analysed for autoantibodies directed against the Fas molecule, and here we report the detection of anti‐Fas autoantibodies. In previous studies, we found autoantibodies directed against DNA topoisomerase I, 3 desmogleins 4 and caspase‐8 5 …”