Detection of Antibodies Against Hog Cholera Virus and Bovine Viral Diarrhea Virus in Porcine Serum

Jensen, Merethe Holm

doi:10.1186/bf03547210

Cited by 99 publications

(9 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The NPLA assay for scFv-Fc fusions and complete IgG antibodies was performed by a modified version of the procedure described by Jensen. 77 All assays were performed in 96-well microtiter plates, with Vero cells used as the host for infection. A dilution series (50 µL) of scFv-Fc fusions or mAbs were incubated with an equal volume of WEE virus strain 160/99, with a TCID 50 /mL of 5 × 10 4 , for 2 h at 37 °C, in 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

Human-like antibodies neutralizing Western equine encephalitis virus

Hülseweh

Rülker

Pelat

et al. 2014

mAbs

View full text Add to dashboard Cite

This study describes the development of the first neutralizing antibodies against Western equine encephalitis virus (WEEV), a member of the genus Alphavirus. WEEV is transmitted by mosquitoes and can spread to the human central nervous system, causing symptoms ranging from mild febrile reactions to life-threatening encephalitis. WEEV has been classified as a biological warfare agent by the US Centers for Disease Control and Prevention. No anti-WEEV drugs are currently commercially available. Neutralizing antibodies are useful for the pre- and post-exposure treatment of WEEV infections. In this study, two immune antibody gene libraries were constructed from two macaques immunized with inactivated WEEV. Four antibodies were selected from these libraries and recloned as scFv-Fc, with a human Fc part. These antibodies bound WEEV specifically in ELISA with little or no cross-reaction with other alphaviruses. They were further analyzed by immunohistochemistry. All binders were suitable for the intracellular detection of WEEV particles. Neutralizing activity was determined in vitro. Three of the four antibodies were found to be neutralizing; about 1 ng/mL of the best antibody (ToR69–3A2) neutralized 50% of 5x104 TCID50/mL. Due to its human-like nature with a germinality index of 89% (VH) and 91% (VL), the ToR69–3A2 antibody is a promising candidate for future passive vaccine development.

show abstract

Section: Methodsmentioning

confidence: 99%

Human-like antibodies neutralizing Western equine encephalitis virus

Hülseweh

Rülker

Pelat

et al. 2014

mAbs

View full text Add to dashboard Cite

show abstract

“…For serum antibody detection, the CTB‐ELISA was used as a complete kit (Wensvoort and others 1988) and the virus neutralisation test was used, as described by Holm‐Jensen (1981).…”

Section: Methodsmentioning

confidence: 99%

Experimental infection of slaughter pigs with classical swine fever virus: transmission of the virus, course of the disease and antibody response

et al. 1999

View full text Add to dashboard Cite

The spread of classical swine fever virus was investigated in an isolation unit containing four pens, each containing six slaughter pigs. One pig in the middle pen of three adjacent pens was inoculated intramuscularly and intranasally with the virus. The fourth pen was located in a separate compartment. The pens were visited in a strict order to study, first, the effect of indirect contact via contaminated clothing and footwear on the spread of the virus to adjacent pens and, secondly, the airborne transmission of the virus between compartments. The pigs were examined and blood samples were taken every other day for 62 days for virological and serological analyses. The virus was highly contagious for the five pigs that were in direct contact with the inoculated pig, but spread to the other pens only after all the pigs in the originally infected pen had become viraemic. The spread of the virus was promoted by contaminated clothing and footwear, but airborne transmission contributed considerably to the spread of the virus within the pighouse. The first clinical signs observed after the virus was introduced into a pen were decreased feed intake, increased mean rectal temperature and apathy. Neither the clinical course of the infection, nor the pattern of seroconversion observed over time, was affected by the differences in the intensity of contact with the virus between the pigs in the different pens.

show abstract

“…Standard gel diffusion precipitin and virus neutralisation tests (Kirkland and Richards 1993) were used to detect antibody to pestivirus. The virus neutralisation test was read after immunoperoxidase staining (Holm Jensen 1981, Hyera and others 1987) and used homologous non‐cytopathogenic virus that had originally been used to infect the donor bull (Kirkland and others 1991).…”

Section: Methodsmentioning

confidence: 99%

Insemination of cattle with semen from a bull transiently infected with pestivirus

et al. 1997

View full text Add to dashboard Cite

When 73 heifers (60 of which were seronegative to pestivirus) were inseminated with pestivirus-contaminated semen from a transiently infected bull, the conception rate to a single insemination was found to be normal (65 per cent). Only three animals became systemically infected, as determined by viraemia and seroconversion. Pestivirus was isolated from the reproductive tracts of two of these heifers when they were slaughtered 42 or 43 days after insemination. Although the initial incidence of infection was low, a cycle of secondary transmission occurred approximately 29 days after insemination, with a further eight heifers (all seronegative) becoming infected from one group of 11 seronegative and four seropositive animals.

show abstract

Detection of Antibodies Against Hog Cholera Virus and Bovine Viral Diarrhea Virus in Porcine Serum

Cited by 99 publications

References 7 publications

Human-like antibodies neutralizing Western equine encephalitis virus

Human-like antibodies neutralizing Western equine encephalitis virus

Experimental infection of slaughter pigs with classical swine fever virus: transmission of the virus, course of the disease and antibody response

Insemination of cattle with semen from a bull transiently infected with pestivirus

Contact Info

Product

Resources

About