Detection of Antibodies to Sendai Virus in Mouse Sera by Enzyme-Linked Immunosorbent Assay (Elisa)

Suzuki, Emiko; Noguchi, Akira; Takeda, Hiroyasu

doi:10.7883/yoken1952.40.109

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…Serological methods, including ELISA, have been employed by different groups to detect anti-SeV antibodies (Ertl et al, 1979;Suzuki et al, 1987;Yanabe and Yoshida, 1986). The quality of antigens coated on plates is the most important factor affecting the accuracy of ELISA.…”

Section: Discussionmentioning

confidence: 99%

“…Serological methods, such as ELISA, are used commonly to detect antibodies against SeV. SeV complete virions are used as antigens for these methods (Ertl et al, 1979;Parker et al, 1979;Suzuki et al, 1987). However, the complexity of SeV virion preparations can lead to cross-reactivity of sera antibodies with nonspecific antigens (Ito et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

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Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array

Tong

Qin

et al. 2013

Journal of Virological Methods

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Sendai virus (SeV) is strictly monitored in laboratory rodents. Currently, complete virions have been used as antigens in SeV serological tests. However, the complexity of SeV virion antigen limits the accuracy of the diagnostic method. In the current study, complete SeV virion antigen was separated on SDS-PAGE and analyzed, with nucleocapsid protein (NP) showing predominant antigenicity. A peptide array containing overlapping 14-mer peptides covering the entire NP was developed. The array used SeV positive serum and resulted in four antigenic linear peptides being identified, which were located in the carboxyl-terminus of NP. The four peptides were coated on ELISA plates and tested with SeV positive and SeV negative sera, and the antigenicity of three peptides, NP413-428, NP473-490 and NP507-524, was confirmed. Mixture of the three peptides showed comparable sensitivity and better specificity in clinical rat sera ELISA tests compared with complete SeV virion antigen. In conclusion, the three peptides, NP413-428, NP473-490 and NP507-524, would be good candidates as linear antigens for SeV detection.

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Section: Discussionmentioning

confidence: 99%