Detection of apoptotic cells in archival tissue from diffuse astrocytomas using a monoclonal antibody to single-stranded DNA

Korkolopoulou, Penelope; Konstantinidou, Anastasia; Patsouris, Efstratios; Christodoulou, Panayiotis; Thomas-Tsagli, Euphemia; Davaris, P

doi:10.1002/1096-9896(2000)9999:9999<::aid-path812>3.0.co;2-w

Cited by 27 publications

(16 citation statements)

References 23 publications

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“…Generally, the TUNEL method tends to detect necrotic cells, as well as apoptotic cells, and not to label the early stage apoptosis. Besides, ssDNAimmunostaining was reported to detect the early apoptotic status without a non-specific reaction for necrotic cells [6,13,28]. Watanabe et al [28] described that the positive Fig.…”

Section: Discussionmentioning

confidence: 99%

“…As a role, the detection of fragmented DNA in the tissue sections has been used by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling (TUNEL) assay. Recently, the immunohistochemical method using an antisingle stranded DNA (ssDNA) antibody has been applied to detect apoptotic nuclei in situ [13,28,29]. This antibody specifically recognizes DNA 3'-OH ends, which are fragmented single-stranded DNA.…”

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confidence: 99%

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Lymphoid Apoptosis in Acute Canine Distemper

et al. 2004

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ABSTRACT. The relationship between the canine distemper virus (CDV) infection and apoptosis in the canine lymphoid tissues was investigated using immunostaining for single stranded DNA (ssDNA), TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method, and electron microscopy. Twenty-six lymphoid tissues from 8 spontaneously CDV-infected dogs and 1 non-infected dog were used, and lesions were classified into 4 groups according to frequency of the CDV-antigen. Histologically, the degree of lymphoid depletion tended to depend on amount of CDV antigen. The numbers of ssDNA-and TUNEL-labeling cells were significantly high in the lymphoid tissues with abundant viral antigen. However, ssDNA-and TUNEL-positive lymphocytes were also frequently found even in the lymphoi d tissues where there was only a small amount of CDV-antigen in sinus histiocytes. The incidence and distribution of apoptotic cells in the CDV-antigens-negative lymphoid tissues from infected dogs were equal to those from a non-infected dog. Double labeling immunostaining using a ssDNA and a CDV nucleocapsid protein (CDV-NP) antibody revealed that there were ssDNA positive but CDV-NP negative cells besides those stained doubly positive. Ultrastructurally, lymphocytes in the CDV-infected lymphoid tissues frequ ently had characteristic morphological features of apoptosis such as apoptotic bodies. All these results suggest that CDV leads to lymphocytic apoptosis directly or indirectly, resulting in severe lymphoid depletion and immunosuppression in acute or subacute phase of CDV in fection. KEY WORDS: apoptosis, canine distemper virus, immunosuppression, ssDNA, TUNEL method.

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Section: Discussionmentioning

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confidence: 99%

Lymphoid Apoptosis in Acute Canine Distemper

et al. 2004

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“…More than 1000 hepatocytes were counted in several fields ( 400). The apoptotic index was calculated as the percentage of immunoreactive nuclei out of the total number of hepatocytes counted [19].…”

Section: Preparation Of Paramylon: Paramylon Isolated Frommentioning

confidence: 99%

Hepatoprotective Effects of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z, on Acute Liver Injury Induced by Carbon Tetrachloride in Rats

Sugiyama

Suzuki²,

Mitra³

et al. 2009

J. Vet. Med. Sci.

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ABSTRACT. Paramylon is a -(1-3)-D-glucan isolated from Euglena gracilis Z. This study was designed to evaluate the protective effects of paramylon on liver injury induced by carbon tetrachloride (CCl 4 ) in rats. Wistar stain male rats were orally administered paramylon (500, 1,000 and 2,000 mg/kg body weight) before treatment with a single intraperitoneal dose of 50% CCl 4 (2 ml/kg body weight). The rats were sacrificed 24 hr later, and blood samples were collected for assay of serum biochemical parameters. The livers were excised to evaluate the activity of antioxidant enzymes. Histopathological examination of the livers was also performed. The results showed that the treatment of paramylon prevented elevation of the serum levels of hepatic enzyme markers and inhibited fatty degeneration and hepatic necrosis induced by CCl 4 . Pre-administration of paramylon reduced the liver apoptotic index. The treatment of paramylon recovered reductions of activity of hepatic superoxide dismutase, catalase and glutathione peroxidase induced by CCl 4 . These results demonstrate that paramylon exhibits protective action on acute hepatic injury induced by CCl 4 via an antioxidative mechanism. To the best of our knowledge, this is the first report of a hepatoprotective effect based on the antioxidative action of paramylon.

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“…[19][20][21][22] Immunohistochemical staining of a-SMA was performed as described previously. 23 A DAKO EnVision system (Dako Cytomation) was used for collagen type I, fibronectin, and ssDNA immunostaining.…”

Section: Histological Examinationmentioning

confidence: 99%

Inhibition of transforming growth factor β decreases pancreatic fibrosis and protects the pancreas against chronic injury in mice

Nagashio

Ueno

Imamura

et al. 2004

Laboratory Investigation

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Transforming growth factor-b (TGF-b) is an important cytokine in the fibrogenesis in many organs, including the pancreas. Using an adenoviral vector expressing the entire extracellular domain of type II human TGF-b receptor (AdTb-ExR), we investigated whether inhibition of TGF-b action is effective against persistent pancreatic fibrosis, and whether it exerts a beneficial effect on the pancreas in the process of chronic injury. To induce chronic pancreatic injury and pancreatic fibrosis, mice were subjected to three episodes of acute pancreatitis induced by six intraperitoneal injections of 50 lg/kg body weight cerulein at hourly intervals, per week for 3 consecutive weeks. Mice were infected once with AdTb-ExR, or with a control adenoviral vector expressing bacterial b-galactosidase (AdLacZ). Pancreatic fibrosis was evaluated by histology and hydroxyproline content. Activation of pancreatic stellate cells (PSCs) was assessed by immunostaining for a-smooth muscle actin. Apoptosis and proliferation of acinar cells were assessed by immunostaining of ssDNA and Ki-67, respectively. Three-week cerulein injection induced pancreatic fibrosis and pancreatic atrophy with proliferation of activated PSCs. In AdTb-ExR-injected mice, but not AdLacZ-injected mice, pancreatic fibrosis was significantly attenuated. This finding was accompanied by a reduction of activated PSCs. AdTb-ExR, but not AdLacZ, significantly increased pancreas weight after chronic pancreatic injury. AdTb-ExR did not change the proportion of proliferating acinar cells, whereas it reduced the number of apoptotic acinar cells. Our results demonstrate that inhibition of TGF-b action not only decreases pancreatic fibrosis but also protects the pancreas against chronic injury by preventing acinar cell apoptosis.

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Detection of apoptotic cells in archival tissue from diffuse astrocytomas using a monoclonal antibody to single-stranded DNA

Cited by 27 publications

References 23 publications

Lymphoid Apoptosis in Acute Canine Distemper

Lymphoid Apoptosis in Acute Canine Distemper

Hepatoprotective Effects of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z, on Acute Liver Injury Induced by Carbon Tetrachloride in Rats

Inhibition of transforming growth factor β decreases pancreatic fibrosis and protects the pancreas against chronic injury in mice

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