Detection of arginase through the optical behaviour of liquid crystals due to the pH-dependent adsorption of stearic acid at the aqueous/liquid crystal interface

Duong, Duong Song Thai; Jang, Chang‐Hyun

doi:10.1016/j.snb.2021.129906

Cited by 22 publications

(7 citation statements)

References 31 publications

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“…In this study, an LC-based assay was developed to detect CHL based on the change in the orientation of LCs in response to the electrostatic interaction between DOPG and PRO and the enzymatic reaction between PRO and PAP. According to previous reports [ 19 , 20 ], PAP activity was inhibited in the presence of CHL. We hypothesized that, in the absence of CHL, PAP hydrolyzes PRO into protein fragments, preventing the electrostatic binding of PRO to the DOPG layer.…”

Section: Resultssupporting

confidence: 52%

“…When the system is immersed in the analytical solution, a change in pH or a physical or chemical reaction will result in a change in the orientation of the liquid crystal. [ 19 , 22 – 24 ]. As the orientation of LCs rapidly responds to analytes at high sensitivity and spatial resolution, surfactant-laden aqueous/LC interfaces have been widely used in LC-based sensing systems to detect enzymatic activity [ 20 , 25 – 27 ] and biomolecular interactions [ 15 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Determination of chlorothalonil levels through inhibitory effect on papain activity at protein-decorated liquid crystal interfaces

Duong

Jang

2022

Microchim Acta

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A liquid crystal (LC)-based assay was developed to detect chlorothalonil (CHL). The detection principle is based on (i) the electrostatic interaction between the positively charged protein protamine (PRO) with the negatively charged phospholipid dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DOPG) and (ii) the CHL-mediated inhibition of papain (PAP) activity. The aqueous/LC interface was decorated with a monolayer of DOPG and PRO that self-assembled via electrostatic interactions. PAP can hydrolyze PRO, resulting in the realignment of an LC by DOPG, inducing a shift in the LC response from bright to dark. The addition of CHL can inhibit the activity of PAP, leading to the attraction of PRO to DOPG and the consequent disruption of the LC orientation. The orientation change of the LC in the presence or absence of CHL can be observed from the changes in its optical appearance using a polarized light microscope. Under optimal conditions, the developed assay achieved a detection limit of 0.196 pg mL −1 within a range of determination of 0.65–200 pg mL −1 . The selectivity of the assay was verified in the presence of carbendazim and imidacloprid. The practical application of the proposed assay was demonstrated by its use to determine the levels of CHL in food extracts and environmental samples, which yielded recoveries and relative standard deviations (RSD) in the ranges of 87.39–99.663% and 1.03–6.32%, respectively. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05396-1.

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Section: Resultssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Determination of chlorothalonil levels through inhibitory effect on papain activity at protein-decorated liquid crystal interfaces

Duong

Jang

2022

Microchim Acta

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“…The amphiphilic molecules are protonated and randomly dispersed in the LC phase under an acidic medium. 75,76 In this case, the orientation of LC is planar, and the bright image is observed under cross-polarizers. On the other hand, the amphiphilic molecules are deprotonated under basic conditions in a way that causes them to align at LC/aqueous interface with hydrophobic tails perpendicular to the interface thereby resulting into homeotropic alignment of LC molecules.…”

Section: Resultsmentioning

confidence: 97%

Liquid crystal droplet design by using pseudopeptidic bottlebrush polymer additives

et al. 2022

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“…The correlation coefficients are 0.993 and 0.995, respectively. The LOD was then calculated to be 0.98 wt % for red wine and vodka …”

Section: Resultsmentioning

confidence: 99%

“…The LOD was then calculated to be 0.98 wt % for red wine and vodka. 41 ■ CONCLUSIONS Methanol is a toxic material usually available in homemade or industrial wines. An unsafe amount of methanol in alcoholic spirits could pose numerous adverse effects on people's health.…”

Section: T H Imentioning

confidence: 99%

Rapid and Label-Free Methanol Identification in Alcoholic Beverages Utilizing a Textile Grid Impregnated with Chiral Nematic Liquid Crystals

et al. 2022

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Methanol contamination of alcoholic drinks can lead to severe health problems for human beings including poisoning, headache, blindness, and even death. Therefore, having access to a simple and inexpensive way for monitoring beverages is vital. Herein, a portable, low cost, and easy to use sensor is fabricated based on the exploitation of chiral nematic liquid crystals (CLCs) and a textile grid for detection of methanol in two distinct alcoholic beverages: red wine and vodka. The working principle of the sensor relies on the reorientation of the liquid crystal molecules upon exposure to the contaminated alcoholic beverages with different concentrations of methanol (0, 2, 4, and 6 wt %) and the changes in the observed colorful textures of the CLCs as well as the intensity of the output light. The proposed sensor is label free and rapid.

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Detection of arginase through the optical behaviour of liquid crystals due to the pH-dependent adsorption of stearic acid at the aqueous/liquid crystal interface

Cited by 22 publications

References 31 publications

Determination of chlorothalonil levels through inhibitory effect on papain activity at protein-decorated liquid crystal interfaces

Determination of chlorothalonil levels through inhibitory effect on papain activity at protein-decorated liquid crystal interfaces

Liquid crystal droplet design by using pseudopeptidic bottlebrush polymer additives

Rapid and Label-Free Methanol Identification in Alcoholic Beverages Utilizing a Textile Grid Impregnated with Chiral Nematic Liquid Crystals

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