Detection of aster yellows phytoplasma in false flax based on PCR and RFLP

Khadhair, A. H.; Tewari, J. P.; Howard, R. J.; Paul, V. H.

doi:10.1078/0944-5013-00100

Cited by 17 publications

(9 citation statements)

References 26 publications

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“…For the past decade, the application of molecular techniques has become the most reliable approach in characterizing and studying the phylogenetic relationships of phytoplasmas. Among these techniques, polymerase chain reaction (PCR) is considered the most sensitive method for phytoplasma identification and classification (Ahrens and Seemüller, 1992; Namba et al., 1993; Schneider et al., 1993; Gundersen et al., 1994a; Khadhair et al., 2001). Development of universal and specific primers based on conserved regions of 16S rDNA, 16S–23S rDNA, ribosomal protein and other conserved chromosomal genes or DNA sequences has facilitated the detection and classification of a number of unknown phytoplasmas by PCR assay (Deng and Hiruki, 1991; Lee et al., 1993, 1994; Gundersen et al., 1994b; Kirkpatrick et al., 1994; Leifting et al., 1996).…”

Section: Introductionmentioning

confidence: 99%

Detection, Identification and Molecular Characterization of a Phytoplasma Associated with Arabian Jasmine (Jasminum sambac L.) Witches’ Broom in Oman

Al-Zadjali¹,

Natsuaki²,

Okuda³

2007

Journal of Phytopathology

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Arabian jasmine (Jasminum sambac L.) plants showing witchesÕ broom (WB) symptoms were found in two regions in the Sultanate of Oman. Polymerase chain reaction (PCR) amplification of the 16S rRNA gene and the 16S-23S spacer region utilizing phytoplasmaspecific universal and designed primer pairs, and transmission electron microscopy of phytoplasma-like structures in phloem elements confirmed phytoplasma infection in the symptomatic plants. PCR products primed with the P1/P7 primer pair were 1804 bp for jasmine witchesÕ broom (JasWB) and 1805 bp for alfalfa (Medicago sativa L.) witchesÕ broom (AlfWB). Actual and putative restriction fragment length polymorphic analysis indicated that jasmine and AlfWB phytoplasmas were molecularly indistinguishable from each other and closely related to papaya yellow crinkle (PYC), as well as being distinct from lime WB (LWB) and Omani alfalfa WB (OmAlfWB) phytoplasmas. A sequence homology search of JasWB and AlfWB showed 99.8% similarity with PYC from New Zealand and 99.6% similarity with each other (JasWB/AlfWB). The jasmine and AlfWB phytoplasmas were also shown to be related to the peanut WB group (16SrII) of 16S rRNA groups based on a phylogenetic tree generated from phytoplasma strains primed with the P1/P7 primer pair and representing the 15 phytoplasma groups.www.blackwell-synergy.com

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Section: Introductionmentioning

confidence: 99%

Detection, Identification and Molecular Characterization of a Phytoplasma Associated with Arabian Jasmine (Jasminum sambac L.) Witches’ Broom in Oman

Al-Zadjali¹,

Natsuaki²,

Okuda³

2007

Journal of Phytopathology

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“…(d) Phytoplasmas * Camelina sativa plants in experimental plots in Alberta, Canada, were found to be infected by the aster yellows phytoplasma disease (Khadhair et al 2001). …”

Section: Response To Other Human Manipulationmentioning

confidence: 99%

The biology of Canadian weeds. 142. Camelina alyssum (Mill.) Thell.; C. microcarpa Andrz. ex DC.; C. sativa (L.) Crantz.

Francis¹,

Warwick²

2009

Can. J. Plant Sci.

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The Biology of Canadian Weeds. 142. Camelina alyssum (Mill.) The most common of the three species in North America, C. microcarpa, arrived in the late 19th century, and subsequently appeared at numerous crop and uncultivated sites across the country, probably in cargo as the railways expanded. Camelina alyssum appeared in the early 20th century at restricted sites on the prairies, mostly in Saskatchewan. All three species have diminished in importance as crop weeds in western Canada over the past few decades. This reduction could be related to increased weed control by herbicides. Herbicide-resistant biotypes have recently been reported in C. microcarpa. Camelina sativa has attracted renewed interest as an oil crop, because of an adaptation to various climatic conditions, low nutrient requirements and resistance to disease and pests. In Europe, where it is now widely grown, it has shown considerable potential in the food, animal feed, nutraceutical, paint, dye, cosmetic and biofuel industries. In North America, it is being grown on a trial basis mainly for its potential as a biofuel in Alberta, Saskatchewan, the Maritime Provinces, and the northern United States of America. Cet article dresse le bilan de l'information connue sur la biologie de trois adventices du groupe des crucife`res : Camelina alyssum, C. microcarpa et C. sativa. Camelina sativa qu'on utilise ou cultive pour son huile depuis plusieurs sie`cles en Europe, a e´te´la premie`re a`atteindre l'Ame´rique du Nord comme mauvaise herbe, vers le milieu du XIX e sie`cle. Elle s'est ensuite e´tendue peu a`peu aux Prairies, surtout dans les cultures, pour parvenir en Colombie-Britannique et jusque dans les Territoires du Nord-Ouest. C. microcarpa, la plus commune des trois espe`ces en sol nord-ame´ricain, a e´te´introduite vers la fin du XIX e sie`cle avant d'apparaıˆtre dans de multiples cultures et terrains incultes du continent, sans doute avec l'expansion du transport des marchandises et du trafic ferroviaire. Camelina alyssum est survenue au de´but du XX e sie`cle a`quelques endroits des Prairies, principalement en Saskatchewan. Les trois espe`ces ont perdu de l'importance en tant que mauvaises herbes dans l'ouest du Canada ces dernie`res de´cennies. On le doit peut-eˆtre a`l'usage accru des herbicides pour lutter contre les adventices. Des biotypes re´sistants aux herbicides ont re´cemment e´te´signale´s pour C. microcarpa. Camelina sativa a suscite´un regain d'inte´reˆt comme ole´agineux, en raison de sa facilite´d'adaptation aux conditions climatiques, de ses faibles exigences nutritives et de sa re´sistance a`la maladie et aux ravageurs. En Europe, ou`on la cultive couramment, cette plante pre´sente un potentiel conside´rable comme culture vivrie`re ou fourrage`re, comme nutraceutique, ainsi que pour les industries de la peinture, des colorants, des cosme´tiques et des biocarburants. En Ame´rique du Nord, on la cultive expe´rimentalement en Alberta, en Saskatchewan, dans les Maritimes et dans le nord des É .-U., principalement en raison de son u...

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“…2). This primer pair has been used previously to amplify the partial 16S RNA gene of a number of phytoplasmas from various groups (Khadhair et al., 2001, 2002, 2003; Lee et al., 1998; Gundersen and Lee, 1996; Kirkpatrick et al., 1994). The other primer pair P3 / P7 produced the expected PCR products of 320 bp for all of the tested samples (Fig.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Aster Yellows Phytoplasma Associated with Valerian and Sowthistle Plants by PCR–RFLP Analyses

Khadhair

Hiruki²,

Deyholos³

2008

Journal of Phytopathology

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In Alberta, Canada, valerian grown for medicinal purposes and sowthistle, a common weed, showed typical aster yellows symptoms. Molecular diagnosis was made using a universal primer pair (P1 ⁄ P7) designed to amplify the entire 16S rRNA gene and the 16 ⁄ 23S intergenic spacer region in a direct polymerase chain reaction (PCR) assay. This primer pair amplified the DNA samples from valerian and sowthistle and reference controls (AY-27, CP, PWB, AY of canola, LWB). They produced the expected PCR products of 1.8 kb, which were diluted and used as templates in a nested PCR. Two primer pairs R16F2n ⁄ R2 and P3 ⁄ P7 amplified the DNA templates giving PCR products of 1.2 and 0.32 kb, respectively. No PCR product was obtained with either set of primers and DNA isolated from healthy plants. Restriction fragment length polymorphism (RFLP) was used to analyse the partial 16S rDNA sequences (1.2 kb) of all phytoplasma DNA samples after restriction with four endonucleases (AluI, HhaI, MseI and RsaI). The restriction patterns of these strains were found to be identical with the RFLP pattern of the AY phytoplasma reference control (AY-27 strain). Based on the RFLP data, the two strains are members of subgroup A of the AY 16Sr1 group. We report here the first molecular study on the association of AY phytoplasmas with valerian and sowthistle plants.

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Detection of aster yellows phytoplasma in false flax based on PCR and RFLP

Cited by 17 publications

References 26 publications

Detection, Identification and Molecular Characterization of a Phytoplasma Associated with Arabian Jasmine (Jasminum sambac L.) Witches’ Broom in Oman

Detection, Identification and Molecular Characterization of a Phytoplasma Associated with Arabian Jasmine (Jasminum sambac L.) Witches’ Broom in Oman

The biology of Canadian weeds. 142. Camelina alyssum (Mill.) Thell.; C. microcarpa Andrz. ex DC.; C. sativa (L.) Crantz.

Molecular Characterization of Aster Yellows Phytoplasma Associated with Valerian and Sowthistle Plants by PCR–RFLP Analyses

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