Detection of B<i>κ</i>-casein variant (<i>CSN3*B</i>) in Burlina dairy cattle by PCR-TTGE

Pacini, Federico; Fellin, Annalisa; Andrighetto, Christian; Zotto, R. Dal; Marchi, Massimo De; Cassandro, Martino; Lombardi, Angiolella

doi:10.1051/dst:2007018

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…First, the HPLC method did not allow the detection and the quantification of some whey protein fractions, such as immunoglobulins and bovine serum albumin, as well as non-protein nitrogen, determining an overestimation of casein index. Second, Pacini et al (2008) highlighted a greater frequency of j-CN B allele in BU cattle breed, which is associated with greater casein content (Vallas et al 2012). De Marchi et al (2009), Bonfatti et al (2011) and Niero et al (2016) reported values of casein index that were similar to the one calculated in the present study.…”

Section: Protein Fractionsmentioning

confidence: 99%

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed^*

Niero

Visentin

Ton

et al. 2016

Italian Journal of Animal Science

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The aim of the present study was to characterise milk of Burlina local cattle breed for traits of technological and nutritional relevance, such as milk coagulation properties (MCP), and protein, major mineral and fatty acid (FA) composition. Burlina is mainly reared in mountain areas of Veneto Region (Italy) and it has been inserted in conservation plans aiming to avoid biodiversity loss and marginal pasture areas abandonment. Eighty-one individual milk samples were collected in four farms. Milk coagulation properties were determined using Formagraph, and protein, mineral and FA composition were analysed in high performance liquid chromatography, inductively coupled plasma optical emission spectrometry and gas chromatography, respectively. Results evidenced good protein percentage (3.38%) and considerable casein content (28.89 mg/mL), as well as a desirable FA profile, with x-6 to x-3 ratio of 4.04. Somatic cell score, averaging 3.13, is a trait that should be enhanced through the improvement of farm management. This would have positive effects on MCP. Among milk minerals, the most and less abundant were K (1493.53 mg/kg) and Mg (110.07 mg/kg), respectively. Overall, herd, parity and lactation stage explained moderate to low variation of the studied traits. Results of the present study could be useful to valorise Burlina local breed and preserve biodiversity in marginal areas. ARTICLE HISTORY

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Section: Protein Fractionsmentioning

confidence: 99%

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed^*

Niero

Visentin

Ton

et al. 2016

Italian Journal of Animal Science

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“…A regional conservation programme was set up since 1980s aiming to contrast the reduction of population size and the increasing of inbreeding (Bittante et al, 1992). More recently, Pretto et al (2009) compared milk production parameters of BUR to that of Italian Holstein Friesian (IHF) breed; Penasa et al (2010) analysed genetic parameters of production and quality traits of BUR and Pacini et al (2008) studied the k-casein variants using polymerase chain reaction-temperature gradient gel electrophoresis (PCR-TTGE) for the same breed. The importance of BUR, from an economical point of view, is related to the production of a typical cheese called Morlacco and, from a rearing point of view, to its frugal characteristic that along with its longevity and better functional performances are of particular interest especially in marginal areas (Pretto et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Temporal Variation in Genetic Diversity and Population Structure of Burlina Cattle Breed

Maretto

Cassandro

2014

Italian Journal of Animal Science

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We analysed the temporal variation of inbreeding, genetic variability and population structure in the Burlina (BUR) cattle breed. A total of 279 individuals were chosen for the analysis representing a period of 19 years (1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010) and analysed using 24 microsatellite markers. A total of 235 alleles were detected in the population with a mean of 9.79±3.91 alleles per locus. In the 19-year period, a stable pattern in the mean number of alleles was found. The mean observed heterozygosity was 0.63 and it was slightly lower than the expected in all birth year groups. Neither an increase nor a decrease in heterozygosity and inbreeding estimates were detected over the years, with the exception of the FIS index which was close to zero in two birth year groups: 2001-2002 and 2006. Absence of bottleneck events was proved and structure analysis revealed an increase in breed complexity over the years and a clear differentiation with the Italian Holstein Friesian cattle breed. Molecular markers were successfully applied in the monitoring of the genetic variability of BUR thus enabling the planning and the application of strategies for the in situ conservation of genetic resources and the improving of breed identity.

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Bayesian inference of genetic parameters for test-day milk yield, milk quality traits, and somatic cell score in Burlina cows

et al. 2010

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The aim of the study was to infer (co)variance components for daily milk yield, fat and protein contents, and somatic cell score (SCS) in Burlina cattle (a local breed in northeast Italy). Data consisted of 13,576 monthly test-day records of 666 cows (parities 1 to 8) collected in 10 herds between 1999 and 2009. Repeatability animal models were implemented using Bayesian methods. Flat priors were assumed for systematic effects of herd test date, days in milk, and parity, as well as for permanent environmental, genetic, and residual effects. On average, Burlina cows produced 17.0 kg of milk per day, with 3.66 and 3.33 percent of fat and protein, respectively, and 358,000 cells per mL of milk. Marginal posterior medians (highest posterior density of 95%) of heritability were 0.18 (0.09-0.28), 0.28 (0.21-0.36), 0.35 (0.25-0.49), and 0.05 (0.01-0.11) for milk yield, fat content, protein content, and SCS, respectively. Marginal posterior medians of genetic correlations between the traits were low and a 95 percent Bayesian confidence region included zero, with the exception of the genetic correlation between fat and protein contents. Despite the low number of animals in the population, results suggest that genetic variance for production and quality traits exists in Burlina cattle.

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Detection of Bκ-casein variant (CSN3B*) in Burlina dairy cattle by PCR-TTGE

Cited by 4 publications

References 14 publications

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed^*

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed^*

Temporal Variation in Genetic Diversity and Population Structure of Burlina Cattle Breed

Bayesian inference of genetic parameters for test-day milk yield, milk quality traits, and somatic cell score in Burlina cows

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Detection of Bκ-casein variant (CSN3*B) in Burlina dairy cattle by PCR-TTGE

Cited by 4 publications

References 14 publications

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed*

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed*

Temporal Variation in Genetic Diversity and Population Structure of Burlina Cattle Breed

Bayesian inference of genetic parameters for test-day milk yield, milk quality traits, and somatic cell score in Burlina cows

Contact Info

Product

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Detection of Bκ-casein variant (CSN3B*) in Burlina dairy cattle by PCR-TTGE

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed^*

Phenotypic characterisation of milk technological traits, protein fractions, and major mineral and fatty acid composition of Burlina cattle breed^*