Detection of Bacillus Spores by Surface-Enhanced Raman Spectroscopy

Farquharson, Stuart; Shende, Chetan; Gift, Alan D.; Inscore, Frank

doi:10.5772/31859

Cited by 8 publications

(12 citation statements)

References 44 publications

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“…While high quality spectra of 10 9 spores/mL B. anthracis-Sterne were repeatedly obtained, sensitivity quickly decreased at lower concentrations, and no signal was obtained at 10 7 spores/mL. While this sensitivity is similar to other work [34], it is considerably less than previous work that included the use of acetic acid, but not the use of a binding peptide, in which 2.2 × 10 5 spores/mL could be measured [22]. At present this loss is partially attributed to the peptide reducing the amount of silver surface available to the DPA molecules.…”

Section: Resultssupporting

confidence: 72%

“…For SERS measurements the capillaries were mounted horizontally on an XY plate reader (Conix Research, Springfield, OR), and in-house software was used to measure five points spaced along the length of the metal-doped sol-gel plug. The five-point relative standard deviation was typically 20% [22], and the reported spectra are the average of these five 1 min measurements. The spot size for both RS and SERS was approximately 300 microns in diameter.…”

Section: Raman and Surface-enhanced Ramanmentioning

confidence: 99%

“…In the past few years we developed a method capable of detecting a few hundred spores on a surface, well below the infectious dose, within 5 minutes using a portable Raman analyzer and a SERS-active capillary to collect the sample [20]. Key to successful measurements was the use of acetic acid to digest the spores and release DPA, which occurs within 1 minute at room temperature [21,22]. The value of this method is predicated on the fact that only two common spore-forming bacteria, Bacillus and Clostridium, contain CaDPA.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Bacillus anthracis Spores Using Peptide Functionalized SERS-Active Substrates

Sengupta

Shende

Farquharson

et al. 2012

International Journal of Spectroscopy

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The need for portable technologies that can rapidly identify biological warfare agents (BWAs) in the field remains an international priority as expressed at the 2011 Biological Weapons Convention. In recent years, the ability of surface-enhanced Raman spectroscopy (SERS) to rapidly detect various BWAs at very low concentrations has been demonstrated. However, in the specific case of Bacillus anthracis, differentiation at the species level is required since other bacilli are common in the environment, representing potential false-positive responses. To overcome this limitation, we describe the use of a peptide attached to the SERS-active metal that selectively binds Bacillus anthracis-Sterne as the target analyte. Using this approach, 109 B. anthracis-Sterne spores/mL produced an intense dipicolinic acid spectrum upon the addition of acetic acid, while the same concentration and treatment of B. cereus and B. subtilis did not.

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Section: Resultssupporting

confidence: 72%

Section: Raman and Surface-enhanced Ramanmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Bacillus anthracis Spores Using Peptide Functionalized SERS-Active Substrates

Sengupta

Shende

Farquharson

et al. 2012

International Journal of Spectroscopy

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“…The joint use of GbRS and SERS opened a new interesting route for investigating the complex physio-chemical processes occurring in biological systems. GbRS and SERS can be particularly useful for studying the response of spores to stressing stimuli and improving the knowledge of underlying mechanisms [14][15][16][17][18][19]29]. In this work, we focused our attention on the spore secretions, which are strongly correlated to the spore state.…”

Section: Gbrs and Sers Advantages And Drawbacksmentioning

confidence: 99%

“…The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. Additional information on the biochemical changes can also be obtained by means of Raman spectroscopy [14][15][16][17] or Surface-Enhanced Raman Spectroscopy (SERS) [18,19]. In particular, a great interest is devoted to SERS due to its high sensitivity and selectivity [20].…”

Section: Introductionmentioning

confidence: 99%

Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure

Camerlingo

Meo²,

Lepore

et al. 2020

Sensors

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Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of π -bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium Parageobacillus thermantarcticus isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and γ -rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli.

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Highly accurate classification of biological spores by culture medium for forensic attribution using multiple chemical signature types and machine learning

et al. 2020

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Detection of Bacillus Spores by Surface-Enhanced Raman Spectroscopy

Cited by 8 publications

References 44 publications

Detection of Bacillus anthracis Spores Using Peptide Functionalized SERS-Active Substrates

Detection of Bacillus anthracis Spores Using Peptide Functionalized SERS-Active Substrates

Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure

Highly accurate classification of biological spores by culture medium for forensic attribution using multiple chemical signature types and machine learning

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