Detection of bacterial ATP by reversed flow-injection analysis with luminescence detection

In vivo early plaque formation on pure titanium and ceramic abutments: a comparative microbiological and SEM analysis

“…Recently, alternative procedures of bacterial count based on indirect estimation tests have been developed. Among them, the ATP method, based on the release of light after reaction with the 1uciferne-Iuciferase complex have been demonstrated to be a fast and reliable system for calculating the degree of bacterial contamination (Blackburn et al 1989;Chen & Cushion 1994;Connolly et al 1993;Miller et al 1992;Selan et al 1992). The advantages of this method, applied to this study of oral microbiology, are the rapidity of this technique and, more important, the ability of this system to calculate the amount of microbial cells in an independent way to media and culture conditions.…”

“…The aim of the present study was to investigate and compare the colonization process of titanium and ceramic abutments by oral microflora in an in vivo system during the first 14 days by means of microbiological methods, both direct count plate and ATP analysis (Blackburn et al 1989;Miller et al 1992), and scanning electron microscopy (SEM). An additional goal was to evaluate the ATP analysis technique as a method for estimating the plaque formation process in the oral cavity.…”

Use of ATP Bioluminescence for Rapid Detection and Enumeration of Contaminants: The Milliflex Rapid Microbiology Detection and Enumeration System

“…Availability of sensitive luminometers as well as many commercial ATP-bioluminescent kits has allowed the development of various protocols and applications in industrial microbiology. Currently, ATP-bioluminescence is an accepted and common technology used to monitor contamination in areas such as food and beverage, ecology, cosmetic, and clinical (Andreotti & Berthold, 1999;Chen & Godwin, 2006;Davidson et al, 1999;Deininger & Lee, 2001;Frundzhyan & Ugarova, 2007;Miller et al, 1992;Nielsen & Van Dellen, 1989;Selan et al, 1992;Yan et al, 2011). 3. Milliflex rapid microbiological detection and enumeration system 3.1 System description RMDS offers a way to detect and quantify living microorganisms grown on a membrane.…”

Spectroscopic study of the interaction between adenosine disodium triphosphate and gatifloxacin–Al3+ complex and its analytical application

“…The most common methods used for the determination of ATP including bioluminometric (3)(4)(5)(6)(7), resonance light scattering (8), capillary electrophoresis (9), enzyme immobilized biosensor (10), high-performance liquid chromatography (11)(12)(13)(14), luminescence (15,16), electrochemical (17), spectrofluorimetric (18)(19)(20)(21)(22) methods, etc. Some of these methods have suffered from various drawbacks including being time consuming, sample separation and treatment, expensive instruments and reagents, low sensitivity, inconvenient reagent pretreatment, unable to assay real sample etc.…”