Detection of bacterial colonization by the spectral changes of surface-enhanced Raman reporters

Gorbachevskii, M. V.; Filatova, Sofia V.; Filimonova, Alla V.; Копицын, Д. С.; Панченко, А. А.; Винокуров, В. А.; Новиков, А. А.

doi:10.1016/j.bbrc.2021.01.099

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“…Biological samples (e.g., viruses and bacteria) can be analyzed after proper purification but often require complicated spectra processing for interpretation [ 27 , 28 , 29 , 30 ], as the bacterial SERS spectra are not only highly similar but dependent on many factors, with a significant contribution from excreted purine moieties [ 31 ]. Indirect SERS-based approaches can be employed for bacterial colonization monitoring [ 32 ] and cytotoxicity evaluation [ 33 ]. Furthermore, gold–silver nanoparticles may serve as bactericidal agents and SERS substrates, simultaneously providing antimicrobial action and antibiotic-resistant bacteria detection [ 34 ].…”

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confidence: 99%

Antibiotic Susceptibility Testing with Raman Biosensing

et al. 2022

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Antibiotics guard us against bacterial infections and are among the most commonly used medicines. The immediate consequence of their large-scale production and prescription is the development of antibiotic resistance. Therefore, rapid detection of antibiotic susceptibility is required for efficient antimicrobial therapy. One of the promising methods for rapid antibiotic susceptibility testing is Raman spectroscopy. Raman spectroscopy combines fast and contactless acquisition of spectra with good selectivity towards bacterial cells. The antibiotic-induced changes in bacterial cell physiology are detected as distinct features in Raman spectra and can be associated with antibiotic susceptibility. Therefore, the Raman-based approach may be beneficial in designing therapy against multidrug-resistant infections. The surface-enhanced Raman spectroscopy (SERS) and resonance Raman spectroscopy (RRS) additionally provide excellent sensitivity. In this review, we present an analysis of the Raman spectroscopy–based optical biosensing approaches aimed at antibiotic susceptibility testing.

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