2016
DOI: 10.3389/fpls.2016.00279
|View full text |Cite
|
Sign up to set email alerts
|

Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay

Abstract: Genetic engineering offers an attractive alternative in sugarcane breeding for increasing cane and sugar yields as well as disease and insect resistance. Bar transgenic sugarcane employing the herbicide tolerance is a useful agronomical trait in weed control. In this study, a loop-mediated isothermal amplification (LAMP) assay for rapid detection of the bar gene in transgenic sugarcane has been developed and evaluated. A set of six primers was designed for LAMP-based amplification of the bar gene. The LAMP rea… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
9
0

Year Published

2017
2017
2024
2024

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 20 publications
(10 citation statements)
references
References 60 publications
1
9
0
Order By: Relevance
“…Fernández- Soto et al (2014) reported 100% activity of Bst DNA polymerase in the range of 60 °C-65 °C which reaches just 20% beyond 70 °C. Zhou et al (2016) also found that Bst DNA polymerase dosage ranging from 6.0 to 8.0 U showed good results in LAMP reaction. Bst DNA polymerase is a Mg + 2 dependent enzyme, which utilizes magnesium as a chelate with nucleotidyl di-or tri-phosphates or the NTP substrate and the metal cofactor serves as a mediator of phosphoryl or nucleotidyl transfer (Cowan 2002).…”
Section: Discussionmentioning
confidence: 81%
“…Fernández- Soto et al (2014) reported 100% activity of Bst DNA polymerase in the range of 60 °C-65 °C which reaches just 20% beyond 70 °C. Zhou et al (2016) also found that Bst DNA polymerase dosage ranging from 6.0 to 8.0 U showed good results in LAMP reaction. Bst DNA polymerase is a Mg + 2 dependent enzyme, which utilizes magnesium as a chelate with nucleotidyl di-or tri-phosphates or the NTP substrate and the metal cofactor serves as a mediator of phosphoryl or nucleotidyl transfer (Cowan 2002).…”
Section: Discussionmentioning
confidence: 81%
“…For performing LAMP assay on qPCR thermocycler, supernatant collected from heat‐treated serum (described in the sample preparation section) was serially diluted 10‐fold, and 4 μL of 1/100 diluted sample was used to set the LAMP reaction. Real‐time LAMP assay was performed in a thermocycler (StepOne™, Applied Biosystems; USA) using the same reaction mixture described above, except for the addition of 20X EVAGreen™ (Biotium, Inc. CA, USA: Catalog No‐31000) as the intercalating dye . The reactions were subjected to 40 cycles at 63°C for 1 minute followed by 85°C for 5 minute.…”
Section: Methodsmentioning
confidence: 99%
“…Catalog No-31000) as the intercalating dye. 26 The reactions were subjected to 40 cycles at 63°C for 1 minute followed by 85°C for 5 minute. During real-time amplification, the fluorescence data were obtained on the 6-carboxyfluorescein (FAM) channel ( Figure 1D).…”
Section: Lamp Assay Followed By Detection In Agarose Gel and Qpcr Tmentioning
confidence: 99%
See 2 more Smart Citations