Detection of base-pair mismatches in DNA using graphene-based nanopore device

Kundu, Sourav; Karmakar, S. N.

doi:10.1088/0957-4484/27/13/135101

Cited by 13 publications

(6 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A second way of sequencing DNA is to monitor the modulation in the transverse current through a narrow graphene ribbon (or other conductive 2D materials) with a nanopore, on interaction with translocating DNA molecules (Figures b and a). The base-specific interaction between DNA and nanopore (that is, each base type has a characteristic coupling strength with the ribbon) affects the local density of states around the nanopore, thereby modulating the transverse conductance of the nanoribbon. − As a voltage was applied between the ends of the graphene nanoribbon, the induced transverse current through the ribbon could be used to read the DNA sequence.…”

Section: Transverse Current Detectionmentioning

confidence: 99%

Nanopores in Graphene and Other 2D Materials: A Decade’s Journey toward Sequencing

2021

View full text Add to dashboard Cite

Nanopore techniques offer a low-cost, label-free, and high-throughput platform that could be used in single-molecule biosensing and in particular DNA sequencing. Since 2010, graphene and other two-dimensional (2D) materials have attracted considerable attention as membranes for producing nanopore devices, owing to their subnanometer thickness that can in theory provide the highest possible spatial resolution of detection. Moreover, 2D materials can be electrically conductive, which potentially enables alternative measurement schemes relying on the transverse current across the membrane material itself and thereby extends the technical capability of traditional ionic current-based nanopore devices. In this review, we discuss key advances in experimental and computational research into DNA sensing with nanopores built from 2D materials, focusing on both the ionic current and transverse current measurement schemes. Challenges associated with the development of 2D material nanopores toward DNA sequencing are further analyzed, concentrating on lowering the noise levels, slowing down DNA translocation, and inhibiting DNA fluctuations inside the pores. Finally, we overview future directions of research that may expedite the emergence of proof-of-concept DNA sequencing with 2D material nanopores.

show abstract

Section: Transverse Current Detectionmentioning

confidence: 99%

Nanopores in Graphene and Other 2D Materials: A Decade’s Journey toward Sequencing

2021

View full text Add to dashboard Cite

show abstract

“…Previous experimental and theoretical investigations payed much attention to detect charge transport through nucleobases using graphene as electrodes [1,3,7,16,17]. While the effects of metal electrodes are less clear.…”

Section: Introductionmentioning

confidence: 99%

Transverse electronic transport properties of single DNA nucleobase pairs between different electrode materials

Liang

et al. 2023

J. Phys.: Condens. Matter

View full text Add to dashboard Cite

Detection of gene mutation through electronic transport properties measurements is an attractive research topic. For this purpose, we computed the current-voltage characteristics of adenine-thymine and guanine-cytosine nucleobase pairs, using a combination method of density-functional theory with non-equilibrium Green's function. Gene mutation was also simulated by structural change in nucleobase pairs by a double proton transfer mechanism. Four different metal electrodes were tested. Comparing the results, nucleobase pairs between platinum surfaces showed distinct electronic transport properties. Such as reverse rectifying direction and negative differential resistance behaviors. The discrepancy can be explained from series of electronic and structural analyses. All these results made identification of structural changes in individual DNA nucleobase pairs possible.

show abstract

“…Biological nanopores are made of proteins with funnel-shaped structures, such as a-hemolysin [25][26][27][28][29][30][31][32], Msp A [18] and Phi29 [33]. Solid-state nanopores are made of nonbiological materials [34,35], such as silicon nitride [36,37], quartz [38], and graphene [39][40][41][42][43]. Currently, both types of nanopores have shown promise in providing critical information regarding protein characteristics using a small amount of protein.…”

Section: Introductionmentioning

confidence: 99%