Detection of Biofilm Genes (IcaA and IcaD) in Staphylococcus spp.

Sulaiman, Aws I.; Abdulla, Basima A.

doi:10.33899/rjs.2018.159338

Cited by 1 publication

(1 citation statement)

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“…Previous study in Mosul, Iraq showed the species S. hominis, S. epidermidis, S. aureus and S. xylosus contain the gene icaD in 42.8% of the isolates [46]. Another study conducted in Basra, Iraq of 150 isolates of S. aureus showed that 100% of the isolates harboured icaD gene [47].…”

Section: Discussionmentioning

confidence: 99%

The Ability of Staphylococcus aureus to Establish Biofilm on Acrylic, Plastic, and Metallic Denture Materials

Mohammed

Al-Mathkhury²

2023

Iraqi Journal of Science

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Staphylococcus aureus is a common pathogenic agent due to its ability to cause various types of infections, ranging from mild skin infections to sever systemic diseases. One of the most virulence factors of this bacterium is its ability to from biofilms on solid surfaces by anchoring the planktonic cells and by producing a protective layer of extra polymeric substances. Biofilm formation is controlled through many genes. The most important ones are icaA and icaD. Dentures are prosthetic devices that are made of different materials to replace lost teeth. The aim of this study is to examine the ability of different types of denture materials to support the biofilm formation of S. aureus at phenotypic level by detecting bacterial growth on them using crystal violet and scanning electron microscope, as well as genotypic level through detection and estimation of gene icaA and icaD expression. Our findings showed that the denture materials do support biofilm formation and there is elevation in gene expression of icaA and icaD.

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Section: Discussionmentioning

confidence: 99%