Detection of biological threats. A challenge for directed molecular evolution

Petrenko, Valery A.; Sorokulova, Iryna

doi:10.1016/j.mimet.2004.04.004

Cited by 103 publications

(79 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since then, numerous molecular recognition methods have been elaborated for detection of bacteria, most attempting to incorporate speed and simplicity (Madonna et al, 2001;Mansfield and Forsythe, 2000;Meckes and MacDonald, 2003), as reviewed in Petrenko and Sorokulova, 2004. Simplified, rapid identification is especially important for pathogenic food-borne bacteria.…”

Section: Introductionmentioning

confidence: 99%

Landscape phage probes for Salmonella typhimurium

Sorokulova

Olsen

Chen

et al. 2005

Journal of Microbiological Methods

109

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Landscape phage probes for Salmonella typhimurium

Sorokulova

Olsen

Chen

et al. 2005

Journal of Microbiological Methods

109

View full text Add to dashboard Cite

“…Landscape phages have been shown to serve as substitutes for antibodies and detection probes in enzyme-linked immuno assay (ELISA) and biosensors [13][14][15][16][17][18][19][20][21][22][23], as immunogens [24], gene-delivery vehicles [25] and affinity matrices [30,32].…”

Section: Landscape Phages As Substitute Antibodiesmentioning

confidence: 99%

“…Phage probes against biological threat agents, such as Bacillus anthracis spores and Salmonella typhimurium were isolated in a nonbiased multistage selection procedure using immobilized spores or bacteria as a selector [18][19][20]. Binding of the selected phages to their respective targets was characterized by a precipitation test, fluorescence-activated cell sorting, enzyme-linked immunosorbent assay (ELISA), and fluorescent, optical and electron microscopy.…”

Section: Landscape Phages As Detection Probesmentioning

confidence: 99%

See 1 more Smart Citation

Landscape phage as a molecular recognition interface for detection devices

Petrenko

2008

Microelectronics Journal

View full text Add to dashboard Cite

Filamentous phages are thread-shaped bacterial viruses. Their outer coat is a tube formed by thousands equal copies of the major coat protein pVIII. Libraries of random peptides fused to pVIII domains were used for selection of phages probes specific for a panel of test antigens and biological threat agents. Because the viral carrier in the phage borne bio-selective probes is infective, they can be cloned individually and propagated indefinitely without needs of their chemical synthesis or reconstructing. As a new bioselective material, landscape phages combine unique characteristics of affinity reagents and self assembling proteins. Biorecognition layers formed by the phage-derived probes bind biological agents with high affinity and specificity and generate detectable signals in analytical platforms. The performance of phage-derived materials as biorecognition interface was illustrated by detection of Bacillus anthracis spores and Salmonella typhimurium cells. With further refinement, the phage-derived analytical platforms for detecting and monitoring of numerous threat agents may be developed, since phage interface against any bacteria, virus or toxin may be readily selected from the landscape phage libraries. As an interface in the field-use detectors, they may be superior to antibodies, since they are inexpensive, highly specific and strong binders, resistant to high temperatures and environmental stresses.

show abstract

“…Several approaches to the development of antibody-like (and therefore biomimetic) binders have also been described in recent years. These include bioimprinted sol-gel polymers for binding whole yeast cells (83), peptide-based binders (84,85,86) and "affibodies" -non-antibody binders derived from randomizable small protein scaffolds (e.g. staphylococcal protein A) (87,88).…”

Section: Biomimeticsmentioning

confidence: 99%

Methods for Whole Cell Detection of Microorganisms

Brehm-Stecher

2008

ACS Symposium Series

View full text Add to dashboard Cite

Microbes are ubiquitous, and can be found occupying nearly every imaginable niche on Earth. These include organic and inorganic surfaces, interfacial boundaries and within macroscopically solid matrices, such as the pore space of rocks. Because phylogenetically divergent microbes may be visually indistinguishable, understanding the species distribution and ecological significance of environmental microbes requires diagnostic tools that extend beyond simple phenotypic description. Methods for microbial diagnostics can be divided into two broad categories: cellular and acellular. Acellular techniques, such as the polymerase chain reaction or certain immunoassay formats may be effective at detecting molecular, structural or biochemical targets associated with specific cell types, but this information is provided out of its "natural", and arguably most meaningful context -that of the individual microbial cell. In contrast, cellular methods have the potential to preserve an abundance of valuable information. Apart from molecular identity, this includes information regarding cell morphology and other physiological characteristics, cell number and distribution within a sample, and physical or spatial associations with other cell types. The aim of this chapter is to provide an overview of whole cell methods for microbial detection, including both existing approaches and those still in development. The tools described here are expected to find wide application for the detection of microbes on surfaces or within complex matrices across a number of parallel or allied fields, including environmental, food and clinical microbiologies.

show abstract

Detection of biological threats. A challenge for directed molecular evolution

Cited by 103 publications

References 142 publications

Landscape phage probes for Salmonella typhimurium

Landscape phage probes for Salmonella typhimurium

Landscape phage as a molecular recognition interface for detection devices

Methods for Whole Cell Detection of Microorganisms

Contact Info

Product

Resources

About