Detection of Bovine Viral Diarrhoea Virus Infected Cattle – Testing Tissue Samples Derived from Ear Tagging Using an E<b><sup>rns</sup></b>Capture ELISA

Kühne, Sabine; Schroeder, Cornelia; Holmquist, Göran; Wolf, Georg; Horner, Sharon D.; Brem, Gottfried; Ballagi, A.

doi:10.1111/j.1439-0450.2005.00861.x

Cited by 30 publications

(24 citation statements)

References 27 publications

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“…7,13,15,48 IHC analysis of ear notch tissue specimens is particularly attractive because ear tagging and tissue sampling can be undertaken in one step. 7,26 The presence of BVDV RNA can be detected by RT-PCR analysis. Moreover, this technique offers high sensitivity, making it suitable for testing specimens with potentially low quantities of virus, such as bulk milk, pooled samples of serum or plasma from transiently infected, as well as PI animals, or other biological materials.…”

Section: Overview Of Current Diagnostic Testsmentioning

confidence: 99%

Test Strategies in Bovine Viral Diarrhea Virus Control and Eradication Campaigns in Europe

Houe¹,

Lindberg

Moennig³

2006

J VET Diagn Invest

166

140

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Abstract. Several European countries have initiated national and regional control-and-eradication campaigns for bovine viral diarrhea virus (BVDV). Most of these campaigns do not involve the use of vaccines; in Germany, vaccination is used only in states in which it is considered necessary because of high BVDV prevalence. In European countries without organized BVDV control programs, vaccination is commonly used to control BVDV. Diagnostic test strategies are fundamental to all control-and-eradication campaigns; therefore, the purpose of this review is to describe how the available diagnostic tests are combined into test strategies in the various phases of control-and-eradication campaigns in Europe. Laboratory techniques are available for BVDV diagnosis at the individual animal level and at the herd level. These are strategically used to achieve 3 main objectives: 1) initial tests to classify herd status, 2) follow-up tests to identify individual BVDV-infected animals in infected herds, and 3) continued monitoring to confirm BVDVfree status. For each objective or phase, the validity of the diagnostic tests depends on the mode of BVDV introduction and duration of infection in test-positive herds, and on how long noninfected herds have been clear of BVDV. Therefore, the various herd-level diagnostic tools-such as antibody detection in bulk milk or in blood samples from young stock animals, or BVDV detection in bulk milk-need to be combined appropriately to obtain effective strategies at low cost. If the individual diagnostic tests are used with due consideration of the objectives of a specific phase of a BVDV control program, they are effective tools for controlling and eradicating BVDV in regions not using vaccination and where vaccination is a part of the control or eradication program.

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Section: Overview Of Current Diagnostic Testsmentioning

confidence: 99%

Test Strategies in Bovine Viral Diarrhea Virus Control and Eradication Campaigns in Europe

Houe¹,

Lindberg

Moennig³

2006

J VET Diagn Invest

166

140

View full text Add to dashboard Cite

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“…However, there were a few biopsy tools for the livestock trade. For example Kuhne et al (2005) describe two tools (Typifix, Agrobiogen GmbH, Thalmannsdorf, Germany; and FlexoPLUS Geno, Caisley, Bocholt, Germany) to apply a cattle ear tag while taking a tissue sample. Through direct experience in the collection and processing of tissue samples for a large-scale close-kin mark-recapture study for SBT (Bravington et al 2014), it was evident that large cost savings and improvements in data management and security could be made through the design of a more efficient and secure sampling tool.…”

Section: Discussionmentioning

confidence: 99%

A new tool in the toolbox for large-scale, high-throughput fisheries mark-recapture studies using genetic identification

Bradford

Hill

Davies

et al. 2016

Mar. Freshwater Res.

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Abstract. The lack of independently verifiable estimates of catches and fisheries independent estimates of abundance and fishing mortality are major sources of uncertainty in the management of many fisheries. DNA profiling provides the potential to substantially improve the quality of data for assessments and act as an additional deterrent to illegal, unreported, and unregulated (IUU) fishing. Barriers to the implementation of this technology include cost of sample collection and processing, forensic grade quality control, and the ability to apply undetectable tags. We present the results of a comparison of two current and one new (gene tag tool, GTT) sampling techniques, using the highly valued southern bluefin tuna as an example. We demonstrate that fish sampled with two techniques are highly unlikely to be recognised as 'tagged', whereas one technique was easily recognisable after 73 days. The GTT reduced handling before DNA extraction, whereas both other techniques require additional labour, adding to cost and potential contamination of the evidentiary chain. Evidence of cross-contamination in the Whatman FTA Elute samples suggests they may not be as suitable for at-sea field applications. Two of the three sampling techniques are capable of obtaining high quality tissue samples for stock assessment and chain of custody purposes in a cost-effective and unidentifiable manner.

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“…Commonly used methods include virus isolation, different immune based antigen detection assays, such as ELISA or immunohistochemistry (IHC), and reverse transcriptase-polymerase chain reaction (RT-PCR). Virus isolation and AC-ELISA, however may be negatively influenced by maternal antibody, while, IHC and PCR have proved to be effective even in the presence of antibodies (Zimmer et al, 2004;Kuhne et al, 2005;Njaa et al, 2000;Horner et al, 1995).…”

Section: Detection Of Bvdvmentioning

confidence: 99%

Diagnostic Approaches for Detection of Bovine Viral Diarrhea Virus Persistent Infection

Ahmad¹

2014

J. Inf. Mol. Biol.

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Bovine viral diarrhea is one of the most important diseases of cattle which is causing continuous economic losses to the cattle industry primarily due to decreased reproductive performance. Without doubt, direct contact between BVDV persistently infected, and susceptible animals is the most important transmission route of virus. All control programs which are in use in many countries of the world, mainly depend upon the detection of PI animals, eliminating them and preventing their return into the herds. Various diagnostic tests using ear notch biopsies and serum samples are in use with certain advantages and disadvantages. In detecting the BVDV persistent infection, a complete agreement (P value = 1) was observed between Real time RT-PCR, AC-ELISA, VI and IHC. All four assays were found specific but real time RT-PCR was found to be more sensitive. Both, VI and IHC were found labour intensive, as diagnosis may take more than one week to be made. Further peroxidase based IHC interpretation was found to be difficult somewhat due to subjective reasoning. It is generally recognized, that real time RT-PCR is more sensitive for BVDV detection than the other methods employed in the study period. However, use of real time RT-PCR for screening of individual animal cases is cost prohibited. AC-ELISA while relatively less sensitive was shown to has sufficient sensitivity for cost effective identification of PI. The results appear to warrant the use of AC-ELISA on ear notch biopsies for routine diagnosis of PI animals followed by real time RT-PCR on suspicious samples.All copyrights reserved to Nexus® academic publishers

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Detection of Bovine Viral Diarrhoea Virus Infected Cattle – Testing Tissue Samples Derived from Ear Tagging Using an E^rnsCapture ELISA

Cited by 30 publications

References 27 publications

Test Strategies in Bovine Viral Diarrhea Virus Control and Eradication Campaigns in Europe

Test Strategies in Bovine Viral Diarrhea Virus Control and Eradication Campaigns in Europe

A new tool in the toolbox for large-scale, high-throughput fisheries mark-recapture studies using genetic identification

Diagnostic Approaches for Detection of Bovine Viral Diarrhea Virus Persistent Infection

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Detection of Bovine Viral Diarrhoea Virus Infected Cattle – Testing Tissue Samples Derived from Ear Tagging Using an ErnsCapture ELISA

Cited by 30 publications

References 27 publications

Test Strategies in Bovine Viral Diarrhea Virus Control and Eradication Campaigns in Europe

Test Strategies in Bovine Viral Diarrhea Virus Control and Eradication Campaigns in Europe

A new tool in the toolbox for large-scale, high-throughput fisheries mark-recapture studies using genetic identification

Diagnostic Approaches for Detection of Bovine Viral Diarrhea Virus Persistent Infection

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Product

Resources

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Detection of Bovine Viral Diarrhoea Virus Infected Cattle – Testing Tissue Samples Derived from Ear Tagging Using an E^rnsCapture ELISA