Detection of Brucella antigen in the aborted ovine fetal stomach contents using a modified ELISA test

Al-Nima, Th. M.; Badrani, B. A. Al; Al-Farwachi, M. I.

doi:10.33899/ijvs.2010.5577

Cited by 4 publications

(7 citation statements)

References 13 publications

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“…On one hand, the general occurrence of Brucella antibodies in the raw milk samples was 11.6% and 9.7% according to MRT and ELISA, respectively. These findings are harmonious with published similar studies from Pakistan (14), Iraq (10,13,15), and India (16). On the other hand, higher rates of anti-Brucella antibodies were documented in studies in Libya 21% (17), India 27% (18), Egypt 36.4%-56.2% (19), and Iran 45% (20).…”

Section: Discussionsupporting

confidence: 83%

“…Clinical human and animal brucellosis takes overabundance of synonyms like Malta fever, undulant fever, Mediterranean fever, Rock of Gibraltar fever, Neapolitan fever, Crimean fever, Corps disease, contagious abortion, and Bang's disease, and most of these names are still used in different regions of the world. It is one of the most significant zoonotic foodborne diseases in the world, brucellosis, is still present in Iraq, and is causing much distress to the country (2,10,13). This study addresses fast and reliable techniques for screening of Brucella antibodies among sheep and goats raw milk in Erbil Governorate.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, higher rates of anti-Brucella antibodies were documented in studies in Libya 21% (17), India 27% (18), Egypt 36.4%-56.2% (19), and Iran 45% (20). Such variations might be attributed to various factors including the extent of encounters with wildlife (natural reservoirs), seasonality, level of available veterinary care, and testing methodology (10,13).…”

Section: Discussionmentioning

confidence: 99%

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Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Almashhadany¹

2021

IJVS

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This study aimed to evaluate the occurrence of brucellosis in sheep and goats' raw milk samples by detecting anti-Brucella antibodies and Brucella species at Erbil governorate. A total of 320 raw milk samples (150 sheep milk and 170 goats milk) were irregularly collected from dairy females. The overall occurrence of Brucella antibodies in samples of sheep and goats raw milk was 11.6% and 9.7% according to MRT and indirect ELISA, respectively. Isolation of Brucella (B.) species from samples of raw milk was 7.8%. The isolated species of Brucella were B. melitensis and B. abortus. A noticeable increase in frequency during September to November was observed. In conclusion, brucellosis is still a considerable public health threat in the Erbil. Based on the tests performance, the study recommends MRT in standard observing of brucellosis in milk aggregate farms, centers, and dairy manufactories. Customers are also recommended to adequately pasteurize the milk in order to damage this milk-borne pathogen before ingestion or saleable handling.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Almashhadany¹

2021

IJVS

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“…The systematic review identified 17 studies conducted between 1979 and 2019 that reported the prevalence of brucellosis in different animals including sheep, goats, cattle, and/or buffalo. Animals used in the identified studies included those from slaughterhouse (e.g., [2,24,27]), flocks (e.g., [3,7,28]), random animals (e.g., [27,32]), aborted ewes (e.g., [8,10,34], ewes regardless of the abortion or vaccination status (e.g., [29,30], or rams only [35]. The identified studies have focused on the standardized techniques used for collecting and handling blood samples, as well as the diagnostic tests used for classifying infected and uninfected animals.…”

Section: Systematic Reviewmentioning

confidence: 99%

“…Abortion rates in ewes and buffalo flocks affected with brucellosis in Mosul were estimated at 17.6% and 6.7%, respectively [7]. In a more recent study, the abortion rate in two flocks of sheep was 11.7% [8]. Moreover, in two studies conducted on aborted ewes used RBT as a detection method, one study found 18.5% of aborted ewes tested seropositive for brucellosis [9], and the other study reported that 70% of aborted ewes tested seropositive for brucellosis [10].…”

Section: Introductionmentioning

confidence: 99%

Brucellosis in food-producing animals in Mosul, Iraq: A systematic review and meta-analysis

Dahl

2020

PLoS ONE

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Brucellosis is an endemic disease in food-producing animals in Mosul, Iraq. The objectives of the study reported here were: (i) to identify and assess the evidence and knowledge gaps in published studies that have examined brucellosis in different food-producing animals in Mosul, Iraq; using systematic review approach, and (ii) to quantify the seroprevalence of brucellosis in the city using meta-analysis approach. Google Scholar was used as a search engine to track pertinent peer-reviewed research reports. The search was conducted on November 24, 2019. Keywords used were: brucella, animal, Mosul, Iraq. Peer-reviewed published studies, MSc theses, and PhD dissertations written in Arabic or English were included. Duplicate records were removed, and the screening process was conducted at three levels: titles, abstracts, and full-text articles. Identified studies that have reported the seroprevalence of brucellosis were included in a meta-analysis to calculate an overall prevalence. A total of 214 records were initially identified. Seventeen research reports were added from personal contact and qualified articles' references list. Thirty six articles were qualified for review after removing 35 duplicate records, 155 titles, 11 abstracts, and 5 full text articles. Seventeen studies reported the prevalence of brucellosis, 11 studies assessed different serological tests for diagnosis of brucellosis, 9 studies isolated Brucella spp. from animal specimens and/or animal products, and 4 studies assessed vaccination procedures against brucellosis. The overall seroprevalence of brucellosis in food-producing animals in Mosul over a period of 40 years was 14.14%, including 14.46% for sheep, 12.99% for goats, 11.69% for cattle, and 22.64% for buffalo. The study concluded that the disease is evident in the city with increasing trends over the years, buffalo shows high seroprevalence, the degree of agreement of Rose-Bengal test as a screening test is fair compared to more accurate serological tests such as ELISA; and the disease constitutes a public health concern in the city. Additional studies are important to identify the overlooked predisposing factors, estimate the abortion rate attributable to brucellosis in food-producing animals, and evaluate efficacy of vaccination programs in reducing the prevalence of brucellosis and/or abortion rate.

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Detection of Brucella Antibodies of Sheep in Al-Anbar Province by Using Some Serological Tests

Al-Samarrae

2013

Iraqi J. Vet. Med.

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An investigation was carried out to detect anti-Brucella antibodies of sheep in Al-Anbar province by using few serological tests; rose Bengal plate test (RBPT) and tube agglutination test (TAT). A total of 754 sheep blood samples (94 Ram and 660 Ewe) were collected randomly distributed in Al-Anbar province from unvaccinated sheep flocks with different ages. A total percentage of infected sheep depending on RBPT was 10.21% with significant differences at (P< 0.01), it was 9.69% in ewes, whereas in rams it elevated to reach 13.82%, in addition to the disease was higher seroprevalence at > 3 years (12.78%) in both gender with significant differences at (P<0.05) than lower ages. Out of 77 RBPT positive sera, 38(49.35%) were positive using TAT, there was 14 seropositive with significant differences at (P< 0.01). The study reported that brucellosis was distributed in Al-Anbar province with variable percent (3.26% to 21.27%), as well as emphasized on different epidemiological aspects.

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Detection of Brucella antigen in the aborted ovine fetal stomach contents using a modified ELISA test

Cited by 4 publications

References 13 publications

Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Brucellosis in food-producing animals in Mosul, Iraq: A systematic review and meta-analysis

Detection of Brucella Antibodies of Sheep in Al-Anbar Province by Using Some Serological Tests

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