Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay

Ilhan, Ziya; Solmaz, Hasan; Aksakal, Abdulbaki; Gülhan, Timur; Ekin, İsmail Hakkı; Boynukara, Banur

doi:10.4067/s0301-732x2008000200005

Cited by 21 publications

(17 citation statements)

References 22 publications

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“…In this study, 9. It has been reported that the detection limit of the PCR in milk samples range from 10 bacteria/ml [26], 1000 CFU/ml [11], 2.8 × 10 4 CFU/ml [18] to 4.2 × 10 4 CFU/ml [28]. In this study, 1.7 × 10 3 -1.7 × 10 4 CFU/ml of B. melitensis 16 M strain was detected.…”

Section: Discussionmentioning

confidence: 48%

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Molecular detection of <i>Brucella melitensis</i> in sheep and goat milk in Iran

Shakerian¹,

Deo²,

Rahimi³

et al. 2016

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 48%

“…The extraction procedure used in this study had successfully been employed in the detection of B. melitensis DNA in sheep/goat milk [26]. In a PCR study by Hamdy and Amin [27], 39 milk samples were collected from 21 sheep and 18 goats.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of <i>Brucella melitensis</i> in sheep and goat milk in Iran

Shakerian¹,

Deo²,

Rahimi³

et al. 2016

Trop. J. Pharm Res

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“…As the dairy products are controlled before being distributed, it seems that commercial quality control of these products is not sufficient and exact. Therefore, reducing the possibility of being infected by this pathogen by using accurate 4 Arch Clin Infect Dis. In Press(In Press):e12673.…”

Section: Discussionmentioning

confidence: 99%

“…are accumulated in mammary glands and supra-mammary lymph nodes of infected animals and therefore the milk of these animals will be a source of pathogens (4). The clinical features of Brucellosis are not disease-specific; but almost every organ can be affected (5,6).…”

Section: Introductionmentioning

confidence: 99%

Detection of Brucella spp. in Dairy Products by Real-Time PCR

Moslemi¹,

Soltandalal²,

Beheshtizadeh³

et al. 2018

Arch Clin Infect Dis

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Background and Objectives: Brucella is an intracellular gram-negative bacterium that can infect many kinds of mammals like humans, sheep, cattle, etc. Brucellosis is a contagious occupational disease caused by Brucella spp. that affects individuals who have close contact with infected animals. The clinical features of Brucellosis are not disease-specific and almost every organ can be affected. This zoonotic disease is a great health concern and economically important in many countries, such as Iran. The aim of this study was to detect Brucella spp. in pasteurized and non-pasteurized dairy products. Methods: In this study, 208 samples, including goat, sheep, and cow raw and pasteurized milk as well as pasteurized and nonpasteurized cheese, were collected in Tehran province. The DNA was extracted, and then the real-time PCR was used for detection of the Brucella spp. gene. Results:The prevalence of Brucella spp. contamination in the dairy products was: 45.5% in goat's raw milk, 39.1% in non-pasteurized cheese, 27.3% in sheep's raw milk, 26.3% in cow's raw milk, 25% in pasteurized cheese, and 14.7% in pasteurized milk. Conclusions: Rapid and exact detection of pathogens in dairy products is the most significant factor to prevent foodborne diseases. In addition, the real-time PCR assay is sensitive and specific enough to detect a low number of Brucella spp. in dairy products.

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“…After addition of 10 μl of proteinase K (stock 10 mg/ml distilled water) to each mixture, tubes were incubated at 65°C for 1 h (with shaking). Genomic DNA was extracted using the phenol organic method developed in the laboratory (Ilhan et al 2008).…”

Section: Dna Extraction (Bosshard 2006 With Modifications)mentioning

confidence: 99%

Pathology and molecular diagnosis of paratuberculosis of camels

Alharbi

Al-Swailem

Aldubaib

et al. 2011

Trop Anim Health Prod

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Camels are the prime source of meat and milk in many desert regions of the world including Saudi Arabia. Paratuberculosis of camels, locally called Silag, is a serious and invariably fatal disease in the Arabian camel. Six camels were used in this study. Five camels with clinical paratuberculosis were used to study the pathology of the disease and confirm its aetiology. The sixth camel was clinically healthy and used as a control. The camels were examined clinically and bled for haematological and blood chemistry analysis. They were then humanely killed with a high intravenous dose of thiopental sodium (10 mg/kg) for pathological studies as well as obtaining tissues for microbiological and molecular studies. The clinical signs of the disease were emaciation, diarrhoea, alopecia, wry neck and pale mucous membranes. Laboratory diagnosis showed reduced haemoglobin concentration, low haematocrit and high activity of the serum enzyme alanine aminotransferase. Serum creatinine concentration was normal. These results indicated the infected camels were anaemic and the function of their livers was affected. Postmortem examination showed thickened and corrugated intestinal mucosa, enlarged granulomatous mesenteric lymph nodes, miliary and diffuse granulomas in the liver (in four camels), generalized lymph node granulomas (in one camel), splenic granuloma (in one camel) and mediastinal lymph node granuloma (in two camels). Histopathological examination showed diffuse infiltration of macrophages in all organs showing lesions. Ziehl-Neelsen staining of tissue scraping and tissue sections showed masses of acid fast bacilli, except for the spleen. Infection with Mycobacterium avium subsp. paratuberculosis was confirmed by PCR by targeting the IS900 gene.

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Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay

Cited by 21 publications

References 22 publications

Molecular detection of <i>Brucella melitensis</i> in sheep and goat milk in Iran

Molecular detection of <i>Brucella melitensis</i> in sheep and goat milk in Iran

Detection of Brucella spp. in Dairy Products by Real-Time PCR

Pathology and molecular diagnosis of paratuberculosis of camels

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