Detection of Ca2+-induced acetylcholine released from leukemic T-cells using an amperometric microfluidic sensor

Akhtar, Mahmood Hassan; Hussain, Khalil K.; Gurudatt, N.G.; Shim, Yoon‐Bo

doi:10.1016/j.bios.2017.07.003

Cited by 44 publications

(26 citation statements)

References 29 publications

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“…The microfluidic biosensor showed a wide range of detection from 0.7 nM to 1500 mM with a detection limit of 0.6 + 0.1 nM. Moreover, the sensor was stable and able to detect Ach from biological samples [82].…”

Section: Smart Biosensorsmentioning

confidence: 94%

“…However, enzyme-based electro-biochemical sensors are sensitive, costeffective and robust [80,81]. These biosensors are based on the enzymes acetylcholine esterase (AChE) and choline oxidase (ChoX), which lead to the detection of component hydrogen peroxide after reaction with ACh [82]. Current biosensors for ACh detection are based on co-conjugation of two enzymes on the same electrode [83], and they suffer from the problems of low sensitivity, short-term stability and low detection range.…”

Section: Smart Biosensorsmentioning

confidence: 99%

See 1 more Smart Citation

An update on smart biocatalysts for industrial and biomedical applications

Shakya

Nandakumar

2018

J. R. Soc. Interface.

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Recently, smart biocatalysts, where enzymes are conjugated to stimuli-responsive (smart) polymers, have gained significant attention. Based on the presence or absence of external stimuli, the polymer attached to the enzyme changes its conformation to protect the enzyme from the external environment and regulate the enzyme activity, thus acting as a molecular switch. Owing to this behaviour, smart biocatalysts can be separated easily from a reaction mixture and re-used several times. Several such smart polymer-based biocatalysts have been developed for industrial and biomedical applications. In addition, they have been used in biosensors, biometrics and nano-electronic devices. This review article covers recent advances in developing different kinds of stimuli-responsive enzyme bioconjugates, including conjugation strategies, and their applications.

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Section: Smart Biosensorsmentioning

confidence: 94%

Section: Smart Biosensorsmentioning

confidence: 99%

An update on smart biocatalysts for industrial and biomedical applications

Shakya

Nandakumar

2018

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…Since acetylcholine concentration is slightly lower in Alzheimer's patients than in healthy individuals (5.0-7.8 nM vs. 8.2-11.3 nM, respectively), this system is capable to discriminate both populations (Chauhan et al, 2017). In order to overcome the drawbacks related to enzyme immobilization (mainly related to protein leakage) and enhance the sensitivity, Akhtar et al (2017) covalently co-immobilized both enzymes (AChE and ChO). With this approach, the sensitivity was reduced significantly, reporting a detection limit of ca.…”

Section: Use Of Conductive Polymersmentioning

confidence: 99%

Tunable Polymeric Scaffolds for Enzyme Immobilization

Rodriguez‐Abetxuko

Sánchez-deAlcázar

Muñumer

et al. 2020

Front. Bioeng. Biotechnol.

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The number of methodologies for the immobilization of enzymes using polymeric supports is continuously growing due to the developments in the fields of biotechnology, polymer chemistry, and nanotechnology in the last years. Despite being excellent catalysts, enzymes are very sensitive molecules and can undergo denaturation beyond their natural environment. For overcoming this issue, polymer chemistry offers a wealth of opportunities for the successful combination of enzymes with versatile natural or synthetic polymers. The fabrication of functional, stable, and robust biocatalytic hybrid materials (nanoparticles, capsules, hydrogels, or films) has been proven advantageous for several applications such as biomedicine, organic synthesis, biosensing, and bioremediation. In this review, supported with recent examples of enzyme-protein hybrids, we provide an overview of the methods used to combine both macromolecules, as well as the future directions and the main challenges that are currently being tackled in this field.

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“…Ca 2+ -induced ACh from leukemic cells could be monitored electrochemically, which may lead to the development of detection kits for Alzheimer's and Parkinson's diseases. In addition, variations in the levels of Ach disrupts behavior, learning, and sleep [91,92]. In the electrochemical oxidation of ACh, two-electron transfer is involved and leads to choline and acetate as byproducts.…”

Section: L-glutamate (L-mentioning

confidence: 99%

Nanomaterials-Based Electrochemical Sensors for In Vitro and In Vivo Analyses of Neurotransmitters

et al. 2018

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Neurotransmitters are molecules that transfer chemical signals between neurons to convey messages for any action conducted by the nervous system. All neurotransmitters are medically important; the detection and analysis of these molecules play vital roles in the diagnosis and treatment of diseases. Among analytical strategies, electrochemical techniques have been identified as simple, inexpensive, and less time-consuming processes. Electrochemical analysis is based on the redox behaviors of neurotransmitters, as well as their metabolites. A variety of electrochemical techniques are available for the detection of biomolecules. However, the development of a sensing platform with high sensitivity and selectivity is challenging, and it has been found to be a bottleneck step in the analysis of neurotransmitters. Nanomaterials-based sensor platforms are fascinating for researchers because of their ability to perform the electrochemical analysis of neurotransmitters due to their improved detection efficacy, and they have been widely reported on for their sensitive detection of epinephrine, dopamine, serotonin, glutamate, acetylcholine, nitric oxide, and purines. The advancement of electroanalytical technologies and the innovation of functional nanomaterials have been assisting greatly in in vivo and in vitro analyses of neurotransmitters, especially for point-of-care clinical applications. In this review, firstly, we focus on the most commonly employed electrochemical analysis techniques, in conjunction with their working principles and abilities for the detection of neurotransmitters. Subsequently, we concentrate on the fabrication and development of nanomaterials-based electrochemical sensors and their advantages over other detection techniques. Finally, we address the challenges and the future outlook in the development of electrochemical sensors for the efficient detection of neurotransmitters.

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Detection of Ca2+-induced acetylcholine released from leukemic T-cells using an amperometric microfluidic sensor

Cited by 44 publications

References 29 publications

An update on smart biocatalysts for industrial and biomedical applications

An update on smart biocatalysts for industrial and biomedical applications

Tunable Polymeric Scaffolds for Enzyme Immobilization

Nanomaterials-Based Electrochemical Sensors for In Vitro and In Vivo Analyses of Neurotransmitters

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