Detection of calcium phosphate crystals in the joint fluid of patients with osteoarthritis – analytical approaches and challenges

Yavorskyy, Alexander; Hernandez-Santana, Aaron; McCarthy, Géraldine; McMahon, Gillian

doi:10.1039/b716791a

Cited by 95 publications

(87 citation statements)

References 162 publications

(243 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The elevated ESR might reflect their action on innate immunity cells, inducing inflammatory signals and amplification of already generated. Moreover, the increase of BCP concentration correlates with the severity of the disease (Yavorskyy et al, 2008). To provoke an inhibition of crystal deposition or their degradation represents a novel and tempting approach for application in chronic phase which can limit joint damage.…”

Section: Rankl Expression On Oa Neutrophilsmentioning

confidence: 99%

How Important are Innate Immunity Cells in Osteoarthritis Pathology

Dimitrova¹,

Ivanovska²

2012

Principles of Osteoarthritis- Its Definition, Character, Derivation and Modality-Related Recognition

View full text Add to dashboard Cite

Section: Rankl Expression On Oa Neutrophilsmentioning

confidence: 99%

How Important are Innate Immunity Cells in Osteoarthritis Pathology

Dimitrova¹,

Ivanovska²

2012

Principles of Osteoarthritis- Its Definition, Character, Derivation and Modality-Related Recognition

View full text Add to dashboard Cite

“…Both near infrared spectroscopy (NIR) and Fourier-transform infra-red (FTIR) spectroscopy have been utilized for the investigation of synovial fluid chemical composition in disease states but cannot identify individual components of synovial fluid (Esmonde-White, Mandair et al 2009). FTIR uses automated pattern recognition but has the disadvantage that water interferes with certain parts of the spectra which can result in misinterpretation (Yavorskyy, Hernandez-Santana et al 2008). Raman spectroscopy provides a specific noninvasive, non-destructive and reagentless tool for the investigation of biological tissues (Esmonde-White, Mandair et al 2009).…”

Section: Vibrational Spectroscopymentioning

confidence: 99%

“…Raman spectroscopy provides a specific noninvasive, non-destructive and reagentless tool for the investigation of biological tissues (Esmonde-White, Mandair et al 2009). Water does not interfere with Raman spectroscopy and unique spectra are available for biological molecules (Yavorskyy, Hernandez-Santana et al 2008). However it is expensive with fewer library spectra available (Yavorskyy, Hernandez-Santana et al 2008).…”

Section: Vibrational Spectroscopymentioning

confidence: 99%

“…Water does not interfere with Raman spectroscopy and unique spectra are available for biological molecules (Yavorskyy, Hernandez-Santana et al 2008). However it is expensive with fewer library spectra available (Yavorskyy, Hernandez-Santana et al 2008). Synovial fluid aspirates provide an easy source of biomaterial for the investigation of changes in composition and viscosity in early OA (Esmonde-White, Mandair et al 2009).…”

Section: Vibrational Spectroscopymentioning

confidence: 99%

“…Both NIR and FTIR can be used to identify early OA with a classification rate of greater than 95% using the overall chemical composition generated spectral pattern (Esmonde-White, Mandair et al 2009). Background fluorescence especially from proteins can interfere with the spectra (Yavorskyy, Hernandez-Santana et al 2008). Further refinement with drop deposition to allow rough component separation and segregation of impurities in conjunction with Raman spectroscopy can improve the diagnostic potential of vibrational spectroscopy (Esmonde-White, Mandair et al 2009).…”

Section: Vibrational Spectroscopymentioning

confidence: 99%

See 2 more Smart Citations

The Application of Imaging in Osteoarthritis

Hing¹,

Harris²,

Ejindu³

et al. 2012

Principles of Osteoarthritis- Its Definition, Character, Derivation and Modality-Related Recognition

View full text Add to dashboard Cite

Calcification of articular cartilage in human osteoarthritis

Fuerst

Bertrand

Lammers

et al. 2009

Arthritis & Rheumatism

283

236

View full text Add to dashboard Cite

Objective. Hypertrophic chondrocyte differentiation is a key step in endochondral ossification that produces basic calcium phosphates (BCPs). Although chondrocyte hypertrophy has been associated with osteoarthritis (OA), chondrocalcinosis has been considered an irregular event and linked mainly to calcium pyrophosphate dihydrate (CPPD) deposition. The aim of this study was to determine the prevalence and composition of calcium crystals in human OA and analyze their relationship to disease severity and markers of chondrocyte hypertrophy.Methods. One hundred twenty patients with endstage OA undergoing total knee replacement were prospectively evaluated. Cartilage calcification was studied by conventional x-ray radiography, digital-contact radiography (DCR), field-emission scanning electron microscopy (FE-SEM), and synovial fluid analysis. Cartilage calcification findings were correlated with scores of knee function as well as histologic changes and chondrocyte hypertrophy as analyzed in vitro.Results. DCR revealed mineralization in all cartilage specimens. Its extent correlated significantly with the Hospital for Special Surgery knee score but not with age. FE-SEM analysis showed that BCPs, rather than CPPD, were the prominent minerals. On histologic analysis, it was observed that mineralization correlated with the expression of type X collagen, a marker of chondrocyte hypertrophy. Moreover, there was a strong correlation between the extent of mineralization in vivo and the ability of chondrocytes to produce BCPs in vitro. The induction of hypertrophy in healthy human chondrocytes resulted in a prominent mineralization of the extracellular matrix.Conclusion. These results indicate that mineralization of articular cartilage by BCP is an indissociable process of OA and does not characterize a specific subset of the disease, which has important consequences in the development of therapeutic strategies for patients with OA.Osteoarthritis (OA) is the most common joint disorder and is characterized by cartilage loss, new bone formation at the margins of the joints (osteophytes), changes in subchondral bone, and recurrent synovitis. The incidence of OA increases with age. Calcium pyrophosphate dihydrate (CPPD) crystals are known to cause acute attacks of pseudogout in the joints, but crystal deposition has also been reported to be associated with OA (1). Aside from CPPD crystals, basic calcium phosphates (BCPs), such as carbonatesubstituted hydroxyapatite (HA), tricalcium phosphate, and octacalcium phosphate, have been found in the synovial fluid (SF), synovium, and cartilage from patients with OA (2-4). The data concerning the distribution and frequency of their occurrence vary, depending on patient selection and crystal identification methods (5-7). Identification of BCP crystals in OA

show abstract

Detection of calcium phosphate crystals in the joint fluid of patients with osteoarthritis – analytical approaches and challenges

Cited by 95 publications

References 162 publications

How Important are Innate Immunity Cells in Osteoarthritis Pathology

How Important are Innate Immunity Cells in Osteoarthritis Pathology

The Application of Imaging in Osteoarthritis

Calcification of articular cartilage in human osteoarthritis

Contact Info

Product

Resources

About