Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

Saito, Taís B.; Alfieri, A.A.; Wosiacki, Sheila Rezler; Negrão, Fábio Juliano; Morais, Helio S. Autran de

doi:10.1016/j.rvsc.2005.03.002

Cited by 57 publications

(44 citation statements)

References 12 publications

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“…A virúria é considerada uma forma freqüente de excreção do CDV (Shen et al, 1981;Saito et al, 2006b). Estes resultados ratificam que a urina deve ser incluída como material clínico de eleição para a realização do diagnóstico ante mortem da cinomose canina por meio da RT-PCR.…”

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Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados

Negrão

Alfieri

2007

Arq. Bras. Med. Vet. Zootec.

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Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados

Negrão

Alfieri

2007

Arq. Bras. Med. Vet. Zootec.

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“…In order to contribute with both veterinarian clinicians and veterinary scientific literature, we established inclusion criteria that selected mainly animals presented without the classical clinical picture of distemper. The most of the current papers about nervous distemper (clinical, pathological, molecular, and diagnostic aspects) have been working with dogs presented with typical signs of CDV infection such as myoclonus and respiratory/gastrointestinal signs (SHIN et al, 1995;BAUMGÄRTNER, 2000;JÓŽWIK;FRYMUS, 2002;KOUTINAS et al, 2002;GEBARA et al, 2004b;SAITO et al, 2006;LAN et al, 2006;SILVA et al, 2007); however, the clinical diagnosis of distemper encephalomyelitis is often difficult in cases presented without these conventional evidences of CDV infection (TIPOLD; VANDEVELDE; JAGGY, 1992), and in such situations the clinical diagnosis of nervous distemper may be a challenge for the clinicians (TIPOLD; VANDEVELDE; JAGGY, 1992; VANDEVELDE; CACHIN, 1993;BAUMGÄRTNER, 2000;AMUDE et al, 2006c;AMUDE et al, 2007b). Additionally, although distemper in immature dogs has been the most common presentation of CDV infection (BRAUND, 1994;FRISK et al, 1999;KOUTINAS et al, 2002;VITE, 2005), in this investigation distemper encephalomyelitis in mature dogs were presented.…”

Section: Discussionmentioning

confidence: 99%

Clinical syndromes of nervous distemper in dogs initially presented without conventional evidences of CDV infection

2012

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Despite large vaccination practice, canine distemper virus (CDV) is yet an important infectious agent that leads to nervous disease in canine populations worldwide. Unfortunately, the clinical diagnosis of distemper encephalomyelitis is often difficult in cases presented without conventional evidences of CDV infection such as systemic signs and myoclonus. Therefore, the aim of this study was to evaluate the neurological deficits and the clinical syndromes of nervous distemper in dogs suffering from neurological disease admitted in the absence of the conventional evidences of CDV infection. Dogs presented with central nervous disease in which toxic/traumatic or a CDV-free etiology could be excluded ante mortem were prospectively followed up and which that died (natural death or euthanasia, despite medical treatment) and necropsy was carried out were included in this study. Ten out of 35 evaluated dogs were post mortem diagnosed with CDV encephalomyelitis by both CDV RNA detection through RT-PCR assay in nervous tissue and observation of distemper-compatible neuroparenchymal lesions. According to the nervous signs, clinical history, and the age of presentation, the distemper dogs were grouped in three clinical syndromes of CDV encephalomyelitis: i) canine distemper encephalitis in immature dogs (CDEID) (n=3); ii) multifocal distemper encephalomyelitis in mature dogs (MDEMD) (n=6); and iii) old dog encephalitis (ODE)-like syndrome (n=1). The respective nervous deficits expected from each one of the syndromes herein presented were discussed. Key words: Dog, canine distemper virus, distemper, encephalomyelitis, RT-PCR ResumoApesar da prática de vacinação, o vírus da cinomose canina (CDV) ainda é um importante agente infeccioso que leva à doença neurológica na população canina em todo o mundo. Infelizmente, o diagnóstico clínico da encefalomielite pela cinomose é difícil nos casos apresentados sem evidências clínicas convencionais de infecção pelo CDV, tais como sinais sistêmicos e mioclonia. Portanto, o objetivo deste estudo foi avaliar os déficits neurológicos e as síndromes clínicas da apresentação neurológica da cinomose em cães portadores de doença neurológica apresentados na ausência de evidências convencionais de infecção pelo CDV. Foram prospectivamente acompanhados cães que apresentaram doença do sistema nervoso central, onde a abordagem diagnóstica ante mortem excluiu outras afecções que não cinomose (etiologia tóxica, traumática, degenerativa, neoplásica, etc) incluídos neste estudo os animais que vieram a óbito (morte natural ou eutanásia, apesar do tratamento médico) e foram necropsiados. Dez de 35 cães avaliados foram diagnosticados no post-mortem com encefalomielite pelo CDV pela detecção de RNA viral pelo RT-PCR no tecido nervoso e observação de lesões neuroparenquimatosas compatíveis com cinomose. De acordo com os sinais neurológicos, a história clínica e a idade de apresentação, os cães foram agrupados em três síndromes clínicas da forma neurológica da cinomose: i) encefalite pela cinomose ca...

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“…The use of RT-PCR and PCR asseys to detect CDV RNA, and CAdV-1 and CAdV-2 DNA in the urine of dogs with clinical signs of these diseases has been reported (NEGRÃO; ALFIERI; ALFIERI, 2007;HEADLEY et al, 2013). Urine is easier to collect than other body fluids and is useful in the ante mortem diagnosis of distemper and other viral diseases (SAITO et al, 2006;HEADLEY et al, 2013). The current study evaluated the use of urine in RT-PCR and PCR assays for the detection of CDV, CAdV-1, CAdV-2, and CPV-2.…”

Section: Introductionmentioning

confidence: 99%

“…The RT-PCR assay targeted a 287 base pair (bp) fragment of the N gene of CDV (FRISK et al, 1999), with modifications (SAITO et al, 2006). The amplification of CAdV-1 and CAdV-2 viral DNA was performed by using a PCR assay designed to detect the 508 bp and 1,030 bp fragments of the E gene of CAdV-1 and CAdV-2, respectively (HU et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of canine distemper virus (CDV), canine adenovirus A type 1 and 2 (CAdV-1 and CAdV-2), and canine parvovirus type 2 (CPV-2) in the urine of naturally infected dogs

et al. 2014

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Canine distemper virus (CDV) causes systemic, respiratory, cutaneous, and neurological manifestations in dogs and other mammalians. Canine adenovirus A (CAdV) is divided in two types, CAdV-1 and CAdV-2, and cause infectious canine hepatitis and infectious tracheobronchitis, respectively. Canine parvovirus type 2 (CPV-2)-induced enteritis is one of the most common infectious cause of diarrhea in puppies. The aim of this study was to detect the urinary excretion of major viral pathogens that cause systemic infections in dogs. From December 2011 to December 2012, 41 urine samples were collected from dogs that presented systemic clinical signs of infectious diseases. The samples were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) for CDV and by PCR for CAdV-1, CAdV-2, and CPV-2. RT-PCR amplified a partial fragment of the CDV N gene (287 bp) in 15 (36.6%) urine samples. PCR for E gene of CAdV-1 (508 bp) and CAdV-2 (1,030 bp) was positive in 4 (9.8%) and 1 (2.4%) sample, respectively; CPV-2 partial VP2 capsid protein gene (583 bp) was amplified in 6 (14.6%) samples. These results suggest that urine can be used as a clinical sample for the ante mortem diagnosis by molecular tools such as RT-PCR and PCR for CDV, CAdV-1 and 2, and CPV-2 systemic infections in dogs. Urinary excretion might be an important route for maintaining these viruses within the environment, and should be considered as a source of infection for healthy dogs. Key words: Canine viral diseases, viruria, RT-PCR, PCR, coinfections ResumoO vírus da cinomose canina (CDV) é um Morbilivirus que causa manifestações clínicas sistêmicas, respiratórias, cutâneas e neurológicas em cães e outros mamíferos. O adenovírus canino A (CAdV) pode ser diferenciado em dois tipos, CAdV-1 e CAdV-2, e causam hepatite infecciosa canina e traqueobronquite infecciosa, respectivamente. Enterite pelo parvovírus canino tipo 2 (CPV-2) é uma das diarreias infecciosas mais comuns em cães, especialmente em filhotes. O objetivo deste estudo foi detectar a excreção urinária de alguns dos principais vírus que ocasionam infecções sistêmicas em cães. No período de dezembro de 2011 a dezembro de 2012 foram colhidas 41 amostras de urina de cães com sinais clínicos sistêmicos. As amostras foram avaliadas por reação em cadeia da polimerase precedida por transcrição reversa (RT-PCR) para o CDV e por PCR para CAdV-1, CAdV-2 e CPV-2. A RT-PCR

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Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

Cited by 57 publications

References 12 publications

Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados

Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados

Clinical syndromes of nervous distemper in dogs initially presented without conventional evidences of CDV infection

Molecular detection of canine distemper virus (CDV), canine adenovirus A type 1 and 2 (CAdV-1 and CAdV-2), and canine parvovirus type 2 (CPV-2) in the urine of naturally infected dogs

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