Detection of Canine Oral Papillomavirus DNA in Conjunctival Epithelial Hyperplastic Lesions of Three Dogs

Brandes, K.; Fritsche, Jens; Mueller, N.; Koerschgen, B.; Dierig, B.; Strebelow, Günter; Teifke, Jens Peter

doi:10.1354/vp.46-1-34

Cited by 14 publications

(15 citation statements)

References 16 publications

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“…According to the owners of the horse, the horse was often licking and biting his penile SCC and after a few months the mouth lesion appeared. The detection of EcPV2 DNA in this lesion suggests a broad tropism of EcPV2, able to productively infect histologically different mucosal tissues in different anatomical locations, as has been also reported for COPV, infecting dogs and causing both oral and ocular lesions (Brandes et al, 2009). The possibility of transmission from infected to non-infected horses is unknown.…”

Section: Discussionsupporting

confidence: 53%

RETRACTED: EcPV2 DNA in equine squamous cell carcinomas and normal genital and ocular mucosa

Vanderstraeten¹,

Bogaert²,

Bravo³

et al. 2011

Veterinary Microbiology

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Squamous cell carcinoma (SCC) represents the most common malignant tumour of the eye and external genitals in horses. Comparable to humans, papillomaviruses (PV) have been proposed as etiological agents of cancer in horses and recently, Equine papillomavirus type 2 (EcPV2) has been identified in genital SCCs. Hitherto it had never been demonstrated in ocular SCCs. The first goal of this study was to determine the prevalence of EcPV2 DNA in tissue samples from equine genital and ocular SCCs, genital papillomas and penile intraepithelial neoplasia (PIN) lesions, using EcPV2-specific PCR. The second goal was to investigate the possibility of latent EcPV2 infection in the genital and ocular mucosa of healthy horses on swabs obtained from the eye, penis, vulvovaginal region and cervix. EcPV2 DNA was detected in all genital SCCs (17/17), genital papillomas (8/8), PIN lesions (11/11) and ocular SCCs (9/9). In healthy horses, EcPV2 DNA was detected in 43% (17/40) of penile swabs, 53% (9/17) of vulvovaginal swabs, 47% (8/17) of cervical swabs and 57% (32/56) of ocular swabs. This study confirms the presence of EcPV2 DNA in equine genital SCCs. Moreover, we demonstrate for the first time its involvement in other genital lesions and in ocular SCCs and latent EcPV2 infections in normal genital (including cervical) and ocular equine mucosa. The close relatives of EcPV2 are associated to cutaneous lesions, and this virus is not related to high-risk human papillomaviruses causing cervical cancer. Thus, similar viral tropism does not imply close evolutionary relationship.

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Section: Discussionsupporting

confidence: 53%

RETRACTED: EcPV2 DNA in equine squamous cell carcinomas and normal genital and ocular mucosa

Vanderstraeten¹,

Bogaert²,

Bravo³

et al. 2011

Veterinary Microbiology

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“…1,4,5 Vascular endothelial cell tumors of the lateral limbus or the leading edge of the third eyelid constitute 27% of conjunctival tumors in dogs and tend to occur in the nonpigmented conjunctiva in Bassett hounds, springer spaniels and beagles. 8 Canine squamous papilloma is a benign papillary tumor of unknown etiology and makes up 9% of conjunctival tumor biopsies. Older (mean = 11 years), female, Weimaraner, and possibly German shepherd/large-breed dogs may be predisposed to conjunctival melanomas.…”

Section: Incidence and Risk Factorsmentioning

confidence: 99%

Ocular Tumors

Miller¹,

Dubielzig²

2013

Withrow and MacEwen's Small Animal Clinical Oncology

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“…All DNA samples were amplified for CPV detection by using the general primer set-CP4/5 (forward: 5 -ATGGTACARTGGGCATWTGA-3 ; reverse: 5 -GAGGYTGCAACCAAAAMTGRCT-3 ) [34]. The other primer pair, COPV L1+ (5 -CTTGTTTGGGGCTTAAGAGG-3 ) and COPV L1-(5 -TGCAGTGTGTACCTGTCCTG-3 ) [35], was further utilized to specifically detect COPV among the SCC cases and papilloma cases which present with lesions on the oral mucosa. For each reaction, 2.5 µL of DNA, 5 µL of 10× PCR buffer (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), 1 µL of 10 mM dNTP mixture(Invitrogen), 1.5 µL of 50 mM MgCl (Invitrogen), 1 µL of each primer (10 mM), 0.5 µL of Taq DNA polymerase (Invitrogen) and 37.5 µL of PCR-grade water were mixed.…”

Section: Polymerase Chain Reaction (Pcr) For the Detection Of Cpvsmentioning

confidence: 99%

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

Chang

Chen

Haga

et al. 2020

Viruses

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Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.

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Detection of Canine Oral Papillomavirus DNA in Conjunctival Epithelial Hyperplastic Lesions of Three Dogs

Cited by 14 publications

References 16 publications

RETRACTED: EcPV2 DNA in equine squamous cell carcinomas and normal genital and ocular mucosa

RETRACTED: EcPV2 DNA in equine squamous cell carcinomas and normal genital and ocular mucosa

Ocular Tumors

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

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