Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

El-Herte, Rima I.; Araj, George F.; Matar, Ghassan M.; Baroud, Maysa; Kanafani, Zeina A.; Kanj, Souha S.

doi:10.3855/jidc.2340

Cited by 47 publications

(41 citation statements)

References 24 publications

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“…In 2009, a study from Lebanon revealed that 2.5% of E. coli and 7.84% of K. pneumoniae were ESBL producers and carbapenem resistant [antimicrobial brochure at the American University of Beirut Medical Center (AUB-MC)]. Most recently, E. coli and K. pneumoniae with NDM-1 were recovered from Iraqi patients referred for treatment in France [27] and in Lebanon [28]. A summary of the different carbapenemases recovered from patients in Lebanon and its neighboring countries is presented in Table 2.…”

Section: Carbapenemases Producing Enterobacteriaceae Reported From Lementioning

confidence: 98%

“…As of 2009, NDM-1 had spread from India and Pakistan via medical tourists to the UK, USA, France, Germany, Australia and other countries [26]. In the Gulf region, NDM-1 was first reported in Oman [8], Iraq [27] and, more recently, Kuwait [9] and Lebanon [28]. The bacteria carrying this enzyme are Klebsiella pneumoniae, Escherichia coli, Citrobacter freundii, Enterobacter cloacae, Providencia spp.…”

Section: Classificationmentioning

confidence: 99%

See 1 more Smart Citation

The threat of carbapenem-resistant Enterobacteriaceae in Lebanon: An update on the regional and local epidemiology

El-Herte

Kanj

Matar

et al. 2012

Journal of Infection and Public Health

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Section: Carbapenemases Producing Enterobacteriaceae Reported From Lementioning

confidence: 98%

Section: Classificationmentioning

confidence: 99%

The threat of carbapenem-resistant Enterobacteriaceae in Lebanon: An update on the regional and local epidemiology

El-Herte

Kanj

Matar

et al. 2012

Journal of Infection and Public Health

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“…The tested isolates showed the lowest resistance to fosfomycin, polymyxin B, colistin and tigecycline (0, 19.6, 26.8, and 33.9%, respectively), but these antibiotics also have limitations and adverse effects (El-Herte et al, 2012). Although Colistin and polymyxin B seem to be the last treatment choice for these isolates; our finding of colistin and polymyxin B resistant MBL-producers is of major concern.…”

Section: Discussionmentioning

confidence: 84%

Phenotypic and genotypic methods for detection of metallo beta lactamases among carbapenem resistant Enterobacteriaceae clinical isolates in Alexandria Main University Hospital

El-Ghazzawy¹,

Meheissen²,

Younis³

2016

Afr. J. Microbiol. Res.

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Dissemination of carbapenem resistant Enterobacteriaceae (CRE) poses a considerable threat to public health. The aim of the present work was to estimate the prevalence of metallo beta lactamases (MBL) among CRE isolated from Alexandria Main University Hospital, Egypt, to evaluate the performance of different phenotypic methods for the detection of MBL, and to investigate the local antimicrobial sensitivity profile of these isolates. Eighty CRE were tested for MBL production by Etest® MBL MP/MPI, EDTA double disc synergy test, and EDTA combined disc test. All isolates were confirmed as MBL producers by polymerase chain reaction (PCR). Antibiotic sensitivity testing was performed using disc diffusion method. Among the 80 CRE, 56 isolates (70%) were MBL by PCR. Fifty-four isolates were positive for NDM (96.4%). Meropenem EDTA CDT was the most sensitive test (94.6%). Blood was the most frequent sample from which MBL were isolated (51.7%). Majority of the isolates were isolated from intensive care units (82.1%). All MBL were multidrug resistant; Colistin and polymyxin B showed the lowest resistance rate (26.8 and 19.6%, respectively). The EDTA-CDT will provide a reliable, convenient, and cost-effective approach for detection of MBL in laboratories, which cannot afford to perform molecular tests.

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“…Recent studies conducted in Middle East countries also suggests that this region might be an additional reservoir of NDM producers as it has been reported from Oman [13], Lebanon [14], Saudi Arabia [15,16], and Qatar [17].…”

Section: Introductionmentioning

confidence: 99%

“…Molecular detection of carbapenemase genes using polymerase chain reaction (PCR) based methods was found to be quick, accurate and effective in the detection of specific carbapenemases genes, such as KPC, NDM-1, OXA-48, etc. [13][14][15][16][17]. Simple PCR assays targeting a single carbapenemase type have been used successfully in numerous studies.…”

Section: Introductionmentioning

confidence: 99%

Frequency of Carbapenemase Producing Klebsiella pneumoniae in Makkah, Saudi Arabia

Khan¹

2016

Journal of Microbiology and Infectious Diseases

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Objective: Carbapenemase producing Klebsiella pneumoniae is emerging as an important pathogen worldwide which has alarming clinical threat with serious effects on patients' outcome. The aim of our study is to determine prevalence of carbapenemase producing K. pneumoniae and its antibiotic susceptibility pattern in tertiary care hospitals. Methods:A prospective study was performed on 260 clinical isolates of K. pneumoniae collected from five different hospitals of Makkah, Saudi Arabia from January to July 2015. All clinical isolates were confirmed as K. pneumoniae using Vitek 2 system. These isolates were then screened for potential carbapenemase producers by determining reduced susceptibility to carbapenems using representative antibiotic disks of third generation cephalosporins and carbapenems in the disk diffusion test following guidelines of Clinical and Laboratory Standards Institute. Minimum inhibitory concentration of isolates found positive in screening test were determined for cephalosporins, carbapenems, aminoglycosides and tigecycline and colistin using Vitek-2 system. Modified Hodge test was performed to detect carbapenemase production on the isolates suspected for carbapenemase production.Results: Out of 260 K. pneumoniae isolates, 31 (11.9%) have shown 100% resistance to all cephalosporins. Of 31 K. pneumoniae isolates, 15 (48.4%) were found positive for carbapenemase. All carbapenem resistant isolates were sensitive to colistin and tigecycline. However, these isolates have shown resistance to amikacin (41.9%) and gentamicin (51.6%), respectively. Conclusion:This is the first report from Makkah reporting carbapenamse producing K.pneumoniae. Of 12% potential carbapenemase producing K.pneumoniae, 48.4% were found positive for carbapenemase production. Molecular characterization of these strains will help to determine the type of carbapenemase prevalent in this area. J Microbiol Infect Dis 2016;6(3): 121-127

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Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

Cited by 47 publications

References 24 publications

The threat of carbapenem-resistant Enterobacteriaceae in Lebanon: An update on the regional and local epidemiology

The threat of carbapenem-resistant Enterobacteriaceae in Lebanon: An update on the regional and local epidemiology

Phenotypic and genotypic methods for detection of metallo beta lactamases among carbapenem resistant Enterobacteriaceae clinical isolates in Alexandria Main University Hospital

Frequency of Carbapenemase Producing Klebsiella pneumoniae in Makkah, Saudi Arabia

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