Detection of casein kinase II by aggregation-induced emission

Luan, Zhenzhu; Li, Zhao; Liu, Chao; Song, Weiling; He, Peng; Zhang, Xiaoru

doi:10.1016/j.talanta.2019.04.036

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…Following a similar detection principle, AIEgens comprising dissolution-promoting moieties attached to AIE-active units have been employed for the sensitive determination of β-galactosidase, α-amylase, and lipase activities [ 143 , 144 , 145 , 146 ]. Moreover, hydrophilic peptides have been utilized as enzyme-responsive units and water-solubility promoters for the “turn-on” detection of other enzymes, including proteases, casein kinase II, furin, and autophagy-specific enzymes [ 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 ]. Several excellent review articles have extensively covered this topic, focusing on the AIE effect [ 155 , 156 , 157 , 158 ].…”

Section: Fluorescent Assaysmentioning

confidence: 99%

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Deng,

Chang,

Liu

et al. 2023

Biosensors

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Over the past few decades, molecular self-assembly has witnessed tremendous progress in a variety of biosensing and biomedical applications. In particular, self-assembled nanostructures of small organic molecules and peptides with intriguing characteristics (e.g., structure tailoring, facile processability, and excellent biocompatibility) have shown outstanding potential in the development of various biosensors. In this review, we introduced the unique properties of self-assembled nanostructures with small organic molecules and peptides for biosensing applications. We first discussed the applications of such nanostructures in electrochemical biosensors as electrode supports for enzymes and cells and as signal labels with a large number of electroactive units for signal amplification. Secondly, the utilization of fluorescent nanomaterials by self-assembled dyes or peptides was introduced. Thereinto, typical examples based on target-responsive aggregation-induced emission and decomposition-induced fluorescent enhancement were discussed. Finally, the applications of self-assembled nanomaterials in the colorimetric assays were summarized. We also briefly addressed the challenges and future prospects of biosensors based on self-assembled nanostructures.

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Section: Fluorescent Assaysmentioning

confidence: 99%

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Deng,

Chang,

Liu

et al. 2023

Biosensors

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“…54 The combination of AIE molecules and peptides are expected to be used for mechanism analysis/diagnosis/treatment of chemotherapy, drug screening, and anti-cancer. 55 Based on this idea, Zhang and his colleagues designed a new type of fluorescent probe specifically by coupling a hydrophilic peptide sequence (GRRRADDSDDDDD) with a hydrophobic propeller-type AIE fluorescent probe to detect protein kinases. The presence of carboxypeptidase Y (Cpy) can cause the peptide to be hydrolyzed, and the hydrophobic AIEgens exhibits strong TPE fluorescence emission.…”

Section: μM (C) Fluorescence Spectra Of Different Concentrations Of Alkaline Phosphatase (Alp) (D) Different Enzymes Enhance the Fluorescmentioning

confidence: 99%

Peptide-Based AIEgens: From Molecular Design, Stimuli Responsiveness to Biomedical Application

et al. 2022

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Luminogens with Aggregation-Induced Emission (AIEgens) have a wide range of biomedical applications in bioimaging, anti-cancer, antibacterial and other fields owing to its unique photophysical properties. The precise structural design and modification of AIE molecule have aroused great interest in the past years. As a peptides-AIE molecules hybrid material, peptide-based AIEgens generally have better solubility, biocompatibility and lower systemic toxicity. The functional diversity, modularity and portability of peptides provide more possibilities for intelligent structure and functional design of AIEgens. This review summarizes the recent research progress of peptide-based AIEgens nanomaterials, from molecular design, stimuli responsiveness to biomedical application, focusing on the advantages of peptides and AIE molecules conjugates. Finally, a summary of the challenges and opportunities of peptide-based AIEgens nanomaterials for future clinical biomedical applications is given.

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“…Among the different kinds of AIEgens, TPE is most the commonly used for the modification of protease substrates because of its advantages of easy synthesis and functionalization and excellent AIE characteristics [ 41 ]. Zhang et al designed a fluorescent light-up probe for chymase detection.…”

Section: Proteasesmentioning

confidence: 99%

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

et al. 2022

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Enzymes play a critical role in most complex biochemical processes. Some of them can be regarded as biomarkers for disease diagnosis. Taking advantage of aggregation-induced emission (AIE)-based biosensors, a series of fluorogens with AIE characteristics (AIEgens) have been designed and synthesized for the detection and imaging of enzymes. In this work, we summarized the advances in AIEgens-based probes and sensing platforms for the fluorescent detection of enzymes, including proteases, phosphatases, glycosidases, cholinesterases, telomerase and others. The AIEgens involve organic dyes and metal nanoclusters. This work provides valuable references for the design of novel AIE-based sensing platforms.

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Detection of casein kinase II by aggregation-induced emission

Cited by 7 publications

References 31 publications

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Peptide-Based AIEgens: From Molecular Design, Stimuli Responsiveness to Biomedical Application

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

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