Detection of Catechol Using a Biosensor Based on Biosynthesized Silver Nanoparticles and Polyphenol Oxidase Enzymes

Sandeep, S.; Santhosh, Abhirami; Swamy, Ningappa Kumara; Suresh, G.; Melo, Jose Savio

doi:10.4152/pea.201904257

Cited by 21 publications

(5 citation statements)

References 24 publications

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“…As observed in Figure 7 , the current intensity increased linearly with the catechol concentration from 24.9 to 172 µM, following the equation y = (−197.9 ± 8.61) × (−139.98 ± 0.95)(R 2 = 0.9759). The limit of detection (LOD), calculated following the 3·σ/m criterion, was 2.7·10 −6 M for AgNWs-Tyr, whereas the LOD obtained using an AgNPs-Tyr biosensor (prepared under the same conditions) was 3.2 × 10 −5 M. In addition, the sensitivity calculated for the AgNW-Tyr biosensor was 197.9 µA·mM −1 which is one to two orders of magnitude higher than the sensitivity shown by other sensors combining tyrosinase and silver nanoparticles reported in recent papers [ 16 , 43 ]. Some results obtained with biosensors containing AgNPs or AuNPs are collected in Table 1 .…”

Section: Resultsmentioning

confidence: 58%

“…In the last few years, a number of electrochemical biosensors combining phenol oxidases with a variety of materials, such as polymeric matrices [ 5 , 6 ] or nanomaterials, have been developed [ 7 , 8 ]. Carbon nanotubes (CNTs) [ 9 , 10 ] graphene [ 11 , 12 ], carbon fibers [ 13 ], metallic nanoparticles, metal oxide nanoparticles, nanostars [ 14 , 15 , 16 , 17 , 18 ], molecular imprinted polymers [ 19 ], and nanostructured thin films [ 20 , 21 ] have all been shown to be excellent electron mediators. One-dimensional (1D) nanostructures can be an interesting alternative to zero-dimensional (OD) nanoparticles due to their high surface-to-volume ratios.…”

Section: Introductionmentioning

confidence: 99%

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Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

Salvo-Comino

Martín-Pedrosa

Garcı́a

et al. 2021

Sensors

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The integration of nanomaterials as electron mediators in electrochemical biosensors is taking on an essential role. Due to their high surface-to-volume ratio and high conductivity, metallic nanowires are an interesting option. In this paper, silver nanowires (AgNWs) were exploited to design a novel catechol electrochemical biosensor, and the benefits of increasing the aspect ratio of the electron mediator (nanowires vs. nanoparticles) were analyzed. Atomic force microscopy (AFM) studies have shown a homogeneous distribution of the enzyme along the silver nanowires, maximizing the contact surface. The large contact area promotes electron transfer between the enzyme and the electrode surface, resulting in a Limit of Detection (LOD) of 2.7 × 10−6 M for tyrosinase immobilized onto AgNWs (AgNWs-Tyr), which is one order of magnitude lower than the LOD of 3.2 × 10−5 M) obtained using tyrosinase immobilized onto silver nanoparticles (AgNPs-Tyr). The calculated KM constant was 122 mM. The simultaneous use of electrochemistry and AFM has demonstrated a limited electrochemical fouling that facilitates stable and reproducible detection. Finally, the biosensor showed excellent anti-interference characteristics toward the main phenols present in wines including vanillin, pyrogallol, quercetin and catechin. The biosensor was able to successfully detect the presence of catechol in real wine samples. These results make AgNWs promising elements in nanowired biosensors for the sensitive, stable and rapid voltammetric detection of phenols in real applications.

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Section: Resultsmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

Salvo-Comino

Martín-Pedrosa

Garcı́a

et al. 2021

Sensors

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“…Polyphenol oxidase was then immobilized on the decorated electrode. The fabricated biosensor was capable of detecting catechol reproducibly [248] .…”

Section: Biosensors For Hydrogen Peroxide and Phenolic Compoundsmentioning

confidence: 99%

Electroconductive multi-functional polypyrrole composites for biomedical applications

Zare

Agarwal

Zarepour

et al. 2021

Applied Materials Today

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“…Polymer nanocomposites have drawn immense attention of researchers from different fields due to their special properties such as light weight, low oxidation, ease of processability, long term stability and tailorable electrical, mechanical and electrochemical properties 1–5 . The incorporation of nanostructured materials as fillers into conventional polymers imparts extra‐ordinary characteristics that suit photonic and optoelectric applications 6 .…”

Section: Introductionmentioning

confidence: 99%

Graphene nanoribbon doped PVA nanocomposites for improved UVC blocking and luminescence down‐shifting applications

Swamy,

Mohana,

Madhukar

2023

Polymers for Advanced Techs

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Graphene nanoribbon (GNR) is quasi‐one dimensional carbon based nanomaterial, distinct from graphene and less explored nanofiller in optical and optoelectronic applications. Herein flexible poly vinyl alcohol (PVA) nanocomposites of 0, 0.25, 0.5 and 0.75 wt % compositions of GNR were prepared by using solution intercalation technique and characterized by spectroscopic methods to assess their structural and optical characteristics. The characterization of PVA@GNR nanocomposites revealed Vander Waals binding of GNR to PVA chains and also considerable crystallographic changes in PVA matrix upon the incorporation of GNR. The optical investigations showed UV‐visible absorption in the wavelength range of 200–280 and 350–520 nm which enhanced on increase in GNR dopant concentration. The decrease in the optical band gap and Urbach energy was also observed for the nanocomposites with increase in dopant concentrations thereby suggesting the formation of localized states in the forbidden band region. The refractive index, absorption coefficient, extinction coefficient and optical conductivity of PVA nanocomposites showed excellent agreement with the band gap revelations and indicated UVC light blocking properties in the wavelength of 200–280 nm. Further, the GNR incorporated PVA nanocomposites exhibited improved thermal stability, wettability transitions (hydrophilic to near hydrophilic) and excellent luminescent down‐conversion properties with a high Stokes's shift (100 nm) compared to pristine PVA nanocomposites. The study concludes that the proposed PVA@GNR nanocomposites can ideally fit optical and photovoltaic device applications owing to their transparency coupled with UV blocking and luminescence down‐shifting attributes.

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Detection of Catechol Using a Biosensor Based on Biosynthesized Silver Nanoparticles and Polyphenol Oxidase Enzymes

Cited by 21 publications

References 24 publications

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

Electroconductive multi-functional polypyrrole composites for biomedical applications

Graphene nanoribbon doped PVA nanocomposites for improved UVC blocking and luminescence down‐shifting applications

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