Detection of causative agents of tick-borne rickettsioses in Western Siberia, Russia: identification of Rickettsia raoultii and Rickettsia sibirica DNA in clinical samples

Igolkina, Yana; Краснова, Е. И.; Рар, В. А.; Savelieva, M.; Epikhina, Tamara; Tikunov, Artem; Хохлова, Н. И.; Проворова, В. В.; Tikunova, Nina

doi:10.1016/j.cmi.2017.06.003

Cited by 26 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In China, this causative agent has been found in at least 12 tick species on the basis of literatures and sequences in GenBank (Fang et al, ; Guo et al, ; Han et al, ). Importantly, human cases caused by R. raoultii have also been reported in France (Parola et al, ), Poland (Switaj, Chmielewski, Borkowski, Tylewska‐Wierzbanowska, & Olszynska‐Krowicka, ), Russia (Igolkina et al, ) and China (Jia et al, ; Li et al, ). In this study, R. raoultii was identified in Hy.…”

Section: Discussionmentioning

confidence: 97%

Molecular detection of spotted fever group rickettsiae in hard ticks, northern China

Guo

Wang

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Spotted fever group (SFG) rickettsiae are important causative agents of (re)emerging tick‐borne infectious diseases in humans, and ticks play a key role in their maintenance and transmission. In this study, hard ticks were collected from five sampling sites in North China in 2017 and 2018. Of them, Haemaphysalis longicornis, Rhipicephalus microplus and Dermacentor nuttalli were collected from livestock (sheep and goats) and the vegetation, Hyalomma asiaticum from sheep, goats and camels, and Hyalomma marginatum from sheep and goats. The SFG rickettsiae were identified in these ticks by amplifying the partial rrs and complete 17‐kDa genes, with an overall infection rate of 52.9%. In addition, the nearly full‐length rrs and gltA and partial ompA genes were recovered to classify the species of SFG rickettsiae further. Phylogenetic analysis revealed the presence of three human pathogenic species in Hy. asiaticum, Hy. marginatum, Ha. longicornis and De. nuttalli, including two cultured ones (Rickettsia raoultii and Rickettsia aeschlimannii) and one uncultured (Candidatus R. jingxinensis). Furthermore, partial groEL gene was also obtained, and phylogenetic trees were also reconstructed to better understand the genetic relationship with known sequences in each SFG rickettsiae species detected in the current study. Notably, the R. aeschlimannii sequences described in this study were closely related to those from abroad rather than from another part of China, indicating their different origin. However, the R. raoultii and Ca. R. jingxinensis sequences presented close relationship with variants from other parts of China. In sum, our data revealed SFG rickettsiae species in northern China, highlighting the need for surveillance of their infection in local humans.

show abstract

Section: Discussionmentioning

confidence: 97%

Molecular detection of spotted fever group rickettsiae in hard ticks, northern China

Guo

Wang

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Another study from China [ 17 ] identified R. raoultii DNA in clinical samples, apart from positive serological reports in patients from other countries. Based on these findings, R. raoultii is considered a human pathogen [ 26 ]. The observations of the current study indicate the presence of R. raoultii in ticks in the ROK, which warrants further research.…”

Section: Discussionmentioning

confidence: 99%

First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea

et al. 2021

View full text Add to dashboard Cite

Background Rickettsial diseases associated with the spotted fever group constitute a growing number of newly identified Rickettsia pathogens and their tick vectors in various parts of the world. At least 15 distinct tick species belonging to six genera have shown the presence of Rickettsia raoultii. Herein, we report the detection of R. raoultii in ticks from the Republic of Korea (ROK). Methods Thirty-five ticks were collected from 29 patients with tick bites in Gwangju Metropolitan City, Jeollanam Province, ROK. The ticks were identified using molecular, morphological, and taxonomic characteristics. All samples were screened for presence of Rickettsia species using nested polymerase chain reactions of their outer membrane protein (ompA) and citrate synthase (gltA) genes. The amplified products were sequenced for subsequent phylogenetic analyses. Results Sequencing data showed the DNA sequences of R. raoultii in three Haemaphysalis longicornis ticks. All three tick samples were 99.4–100% similar to previously reported partial sequences of ompA of R. raoultii strains CP019435 and MF002523, which formed a single clade with the reference strains. Conclusions We provide the first description and molecular identification of R. raoultii detected in H. longicornis ticks in the ROK. This observation extends the geographical distribution of R. raoultii. Screening of human samples for this pathogen will provide information about the prevalence of rickettsial infections in this region.

show abstract

“…Another study from China [18] identi ed R. raoultii DNA in clinical samples, besides the positive serological reports in patients from other countries. Based on these ndings, R. raoultii has been considered as a human pathogen [27].…”

Section: Discussionmentioning

confidence: 99%

First Molecular Detection of Human Pathogen Rickettsia Raoultii in Ticks from Republic of Korea

Tariq

Seo

Kim

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Rickettsial diseases, associated with the spotted fever group (SFG), constitute a growing number of newly identified rickettsia pathogens and their tick vectors, in various parts of the world. At least 15 distinct tick species owing to six genera have shown the presence of Rickettsia raoultii. Here, we report the detection of R. raoultii in ticks from the Republic of Korea (ROK).Methods: A total of 35 ticks, collected from patients of tick bites in Gwangju Metropolitan City, Jeollanam Province, ROK. The ticks were were identified through their molecular, morphological, and taxonomic characteristics. All samples were screened by nested polymerase chain reactions of their outer membrane protein (ompA) and citrate synthase (gltA) genes. The amplified products were sequenced and their phylogenetic analyses were carried out.Results: Sequencing data showed that the DNA sequences of R. raoultii found in the three H. longicornis ticks. All 3 tick samples were 99.4-100% analogous to the previously reported partial sequences of ompA of R. raoultii strains CP019435 and MF002523, forming a single clade with the reference strains.Conclusions: Present study provides the first description and molecular identification of R. raoultii detected in H. longicornis ticks in ROK. This observation extends the geographical distribution of R. raoultii. Screening of human samples for this pathogen will provide information about the prevalence of rickettsial infection in this region.

show abstract

Detection of causative agents of tick-borne rickettsioses in Western Siberia, Russia: identification of Rickettsia raoultii and Rickettsia sibirica DNA in clinical samples

Abstract: We report the first finding of R. raoultii DNA in clinical samples from Russian patients. The clinical manifestations of this rickettsiosis were nonspecific and differed from those caused by R. sibirica.

Cited by 26 publications

References 12 publications

Molecular detection of spotted fever group rickettsiae in hard ticks, northern China

Molecular detection of spotted fever group rickettsiae in hard ticks, northern China

First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea

First Molecular Detection of Human Pathogen Rickettsia Raoultii in Ticks from Republic of Korea

Contact Info

Product

Resources

About