Detection of ceftiofur and oxytetracycline in oral fluids of swine with a pen‐side competitive ELISA test after intramuscular injection

Meiszberg, Allison M.; Karriker, Locke A.; Zimmerman, Jeff; Irwin, Christa; Coetzee, Johann F.

doi:10.1111/j.1365-2885.2010.01259.x

Cited by 9 publications

(9 citation statements)

References 4 publications

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“…Therefore, oral fluids can be used to detected tetracyclines in pig oral fluid using this novel biochip arraybased immunoassay. Oxytetracycline and ceftiofur in oral fluids of swine was also reported in a previous study 10 . However their assay was qualitative, with results reported as either positive or negative, using a pen-side competitive ELISA.…”

Section: Resultssupporting

confidence: 78%

“…However their assay was qualitative, with results reported as either positive or negative, using a pen-side competitive ELISA. In addition, if the ceftiofur pass from the blood to oral fluid as mentioned the previous study 10 , ceftiofur might also be detected easily with the BABIT in field conditions. The average recovery was 55% in the oral fluids collected from pig farm.…”

Section: Resultsmentioning

confidence: 60%

“…Oral fluid samples were collected from pigs as described in a recent study 10 by hanging 100% cotton rope (Wep Ringing Suplly, Inc., Lake Barrington, IL, USA) in each pen for a minimum for 20 min in the morning. Oral fluids were extracted by wringing the ropes through a manual wringer.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Significant research has also been conducted on the use of oral fluid as a diagnostic medium for detection of some viral infections [7][8][9] . Recently, there is a report 10 on the detection of antibacterials such as ceftiofur and oxytetracycline in swine oral fluids. In this study 10 , ceftiofur and oxytetracycline were qualitatively detected in swine oral fluid as positive or negative using a pen-side competitive ELISA.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, there is a report 10 on the detection of antibacterials such as ceftiofur and oxytetracycline in swine oral fluids. In this study 10 , ceftiofur and oxytetracycline were qualitatively detected in swine oral fluid as positive or negative using a pen-side competitive ELISA. In veterinary diagnostic medicine, oral fluid specimens could potentially be used for detection of some drugs for food safety applications.…”

Section: Introductionmentioning

confidence: 99%

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Domuz Ağız Sıvısında Altı Farklı Grup Antimikrobiyal İlacın Aynı Anda Biochip Array-Based Immunoassay ile Tayini

Oruç

Rumbeiha²,

Ensley³

et al. 2013

Kafkas Univ Vet Fak Derg

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The objectives of this study were 1) to determine whether a biochip array-based immunoassay could be used to detect 6 different group antimicrobials in porcine oral fluids collected under experimental conditions; 2) to determine the feasibility of antimicrobial detection in clean versus dirty oral fluid samples; and 3) to determine if the assay could be used to detect chlortetracycline in oral fluids of swine ingesting a routine diet containing chlortetracycline. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different group six antimicrobials in milk, urine, meat, honey, and feed. The assay had not been investigated for potential use in oral fluids. Following evaluation of different extraction procedures, a centrifugation method was chosen. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in both clean and dirty oral fluid samples. However, tetracycline was not well recovered in dirty samples. Chlortetracycline from tetracycline group was detected in all oral fluid samples collected from the field at concentrations ranging from 176.0-698.6 ppb. In conclusion, this assay can detect all target antibacterials from different groups in clean and dirty oral fluid samples with the exception of tetracycline which was not well-recovered from in dirty samples. Keywords: Antimicrobials, Biochip array-based immunoassay, Oral fluid, Swine Domuz Ağız Sıvısında Altı Farklı Grup Antimikrobiyal İlacın Aynı Anda Biochip Array-Based Immunoassay ile Tayini ÖzetÇalışmanın amacı Biochip array-based immunoassay yöntemi ile 6 farklı grup antibakteriyel ilacın deneysel şartlarda toplanmış domuz ağız sıvısında tespit edilip edilemediğini; temiz ve kirli domuz ağız sularında bu antimikrobiyallerin tespit edilebilirliğini ve bu metotla, rasyonlarında sürekli olarak klortetrasiklin bulunan domuzların ağız sıvılarında klortetrasiklinin tespit edilebilirliğini belirlemektir. Bu metod, aynı anda altı farklı grup antimikrobiyalin süt, idrar, et, bal ve yem numunelerinde miktarlarının belirlenmesinde kullanılmaktadır, ancak ağız sıvılarında tespit edilebilirlikleri henüz araştırılmamıştır. Yapılan farklı ekstraksiyon çalışmalarından sonra bir santrifüj metodu seçildi. Bu metodla hedef ilaç olan norfloksasin, seftifor, florfenikol, streptomisin, taylosin ve tetrasiklin temiz ve kirli domuz ağız sıvısında tespit edildi. Ancak, kirli ağız sularında tetrasiklinin geri kazanımı oldukça düşük olarak elde edildi. Klortetrasiklin çiftlikteki domuzların ağız sıvılarında 176.0-698.6 ppb arasında tespit edildi. Sonuç olarak, bu test ile kirli ağız sıvısında tetrasiklin aranması dışında, altı farklı gruptaki hedef antibakteriyel ilaç domuzların ağız sıvısında kolaylıkla tespit edilebilmektedir.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 60%

Section: Sample Collectionmentioning

confidence: 99%