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Assimilating accurate behavioral events over a long period can be labor-intensive and relatively expensive. If an automatic device could accurately record the duration and frequency for a given behavioral event, it would be a valuable alternative to the traditional use of human observers for behavioral studies. Therefore, the objective of this study was to determine the accuracy in the time spent at the waterer and the number of visits to the waterer by individually housed nursery pigs between human observers scoring video files using Observer software (OBS) and an automatic water meter Hobo (WM, control) affixed onto the waterline. Eleven PIC USA genotype gilts (22 ± 2 d of age; 6.5 ± 1.4 kg of BW) were housed individually in pens with ad libitum access to a corn-based starter ration and one nipple waterer. Behavior was collected on d 0 (day of weaning), 7, and 14 of the trial using 1 color camera positioned over 4 attached pens and a RECO-204 DVR at 1 frame per second. For the OBS method, 2 experienced observers recorded drinking behavior from the video files, which was defined as when the gilt placed her mouth over the nipple waterer. Data were analyzed using nonparametric methods and the general linear model and regression procedures in SAS. The experimental unit was the individual pen housing 1 gilt. The GLM model included the method of observation (WM vs. OBS) and time (24 h) as variables, and the gilt nested within method was used as the error term. Gilts consumed more water (P = 0.04) on d 14 than on d 0. The time of day affected (P < 0.001) the number of visits and the time spent at the waterer regardless of the method. However, the OBS method underestimated (P < 0.001) the number of visits to the waterer (3.48 ± 0.33 visits/h for OBS vs. 4.94 ± 0.33 for WM) and overestimated (P < 0.001) the time spent at the waterer (22.6 ± 1.46 s/h for OBS vs. 13.9 ± 1.43 for WM) compared with WM. The relationship between the 2 methods for prediction of time spent at the waterer and number of visits made by the gilts was weak (R 2 = 0.56 and 0.69, respectively). Collectively, these data indicate that the use of the traditional OBS method for quantifying drinking behavior in pigs can be misleading. Quantifying drinking behavior and perhaps other behavioral events via the OBS method must be more accurately validated. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. ABSTRACT: Assimilating accurate behavioral events over a long period can be labor-intensive and relatively expensive. If an automatic device could accurately record the duration and frequency for a given behavioral event, it would be a valuable alternative to the traditional use of human observers for behavioral studies. Therefore, the objective of this study was to determine the accuracy in the time spent at the waterer and the number of visits to the waterer by individually housed nursery pigs between human observers scoring...

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and ImplicationsThere are currently no analgesic drugs specifically approved for pain relief in livestock by the U.S Food and Drug Administration (FDA). Therefore, the objective of this study was to (1) identify the active hours of sound sows and (2) use this information to watch these active hours of the same sows when lame and after pain mitigation treatments. To avoid confounding injury due to aggression, twelve, clinically normal, mixed-parity, crossbred sows were purchased from a commercial producer in Iowa and housed in individual pens at Iowa State University. One 12 v black and white CCTV camera was affixed above the pen. Video was captured onto a DVR at 10 frames per second in black and white mode. Scoring of video began at 0600 and ended at 1800 and two postures (active and inactive), out of pen or unknown were collected. All behavioral data were expressed as percentages and were subjected to a square root arcsine transformation process to normalize the distribution. Transformed data were analyzed using the PROC MIXED procedure in SAS ® . A P value of P ≤ 0.05 was considered to be significant. No differences were observed for out of pen (P = 0.24) or unknown (P = 0.71) for the sows. There was a difference for the time spent active (P = 0.0003) versus inactive (P = 0.0052) over the 12-h for sows. Sows were most active at 0800 (59 %) and at 1600 (66 %) which corresponded to the feeding schedule. The least active hours were 0600, 0700, and 1700 h respectively. In conclusion the critical active hours for these sows were around feeding schedules. Therefore, it is beneficial to watch from 0800 to 1659 to capture when the sows are most active in their home pen environment.

In this trial sampling technique did impact levels of bacterial contamination present in synovial fluid. The differences in contamination levels were highly variable between sampling techniques, ranging from no bacteria present to bacterial concentrations of 10^6. Needle aspiration proceeded by skinning of the joint resulted in the lowest level of contamination in samples indicating the skin is a major source of contamination and the use of needle aspiration over swab collection significantly reduces contamination levels.