Detection of cellular prion protein in exosomes derived from ovine plasma

Berrone, Elena; Corona, Cristiano; Mazza, Maria; Costassa, Elena Vallino; Faro, Mònica; Properzi, Francesca; Guglielmetti, Chiara; Maurella, Cristiana; Caramelli, Maria; Deregibus, Maria Chiara; Camussi, Giovanni; Casalone, Cristina

doi:10.1099/jgv.0.000291

Cited by 17 publications

(12 citation statements)

References 41 publications

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“…In addition, IFNT induces ISG15 gene expression in jugular blood and liver tissue and several ISGs including ISG15, STAT1, and STAT2 in CL via uterine veins [ 43 , 44 ]. It has been reported that blood also contains exosomes for organ-to-organ communications [ 45 , 46 ]. Results from our studies together with these observations suggest that exosomes including IFNT could be involved in the regulation of ISGs in extra-uterine tissues as well.…”

Section: Discussionmentioning

confidence: 99%

Induction of IFNT-Stimulated Genes by Conceptus-Derived Exosomes during the Attachment Period

et al. 2016

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Biochemical and/or physical communication between the conceptus and the uterine endometrium is required for conceptus implantation to the maternal endometrium, leading to placentation and the establishment of pregnancy. We previously reported that in vitro co-culture system with bovine trophoblast CT-1 cells, primary uterine endometrial epithelial cells (EECs), and uterine flushings (UFs) mimics in vivo conceptus attachment process. To identify molecules in UFs responsible for this change, we first characterized protein contents of UFs from day 17 cyclic (C17) and pregnant (P17) ewes through the use of two dimensional-Polyacrylamide Gel Electrophoresis (2D-PAGE), followed by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) analysis. These analyses identified 266 proteins specific for P17 UFs, from which 172 proteins were identified as exosomal proteins. Among 172 exosomal proteins, 8 proteins that had been identified as exosomal proteins were chosen for further analysis, including macrophage-capping protein (CAPG), aldo-keto reductase family 1, member B1 protein (AKR1B1), bcl-2-like protein 15 (BCL2L15), carbonic anhydrase 2 (CA2), isocitrate dehydrogenase 2 (IDH2), eukaryotic translation elongation factor 2 (EEF2), moesin (MSN), and ezrin (EZR). CAPG and AKR1B1 were again confirmed in P15 and P17 UFs, and more importantly CAPG and AKR1B1, mRNA and protein, were found only in P15 and P17 conceptuses. Moreover, exosomes were isolated from C15, C17, P15, or P17 UFs. Only P15 and P17 exosomes, originated from the conceptus, contained interferon tau (IFNT) as well as CAPG and AKR1B1, and up-regulated STAT1, STAT2, MX1, MX2, BST2, and ISG15 transcripts in EECs. These observations indicate that in addition to endometrial derived exosomes previously described, conceptus-derived exosomes are present in UFs and could function to modify endometrial response. These results suggest that exosomes secreted from conceptuses as well as endometria are involved in cell to cell interactions for conceptus implantation to the maternal endometrium.

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Section: Discussionmentioning

confidence: 99%

Induction of IFNT-Stimulated Genes by Conceptus-Derived Exosomes during the Attachment Period

et al. 2016

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“…Interestingly, it is likely that conceptus secretes not only IFNT itself, but also IFNT incorporated into EVs, both of which act on the uterine endometrium, respectively. It is well documented that blood contains EVs for organ-to-organ communications [ 72 , 73 ]. Recently, extrauterine or endocrine effects of IFNT have also been noted [ 74 , 75 ], suggesting that EVs including IFNT could directly or indirectly function on extrauterine tissues as well.…”

Section: Roles Of Ev Interaction With Progesterone Ifnt and Lipimentioning

confidence: 99%

Emerging Role of Extracellular Vesicles in Embryo–Maternal Communication throughout Implantation Processes

Nakamura

Kusama

Suda

et al. 2020

IJMS

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In ruminants, the establishment of proper conceptus–endometrial communication is essential for conceptus implantation and subsequent successful placentation. Accumulated evidence supports the idea that extracellular vesicles (EVs) present in uterine lumen are involved in conceptus–endometrial interactions during the preimplantation period. EVs make up a new field of intercellular communicators, which transport a variety of bioactive molecules, including soluble and membrane-bound proteins, lipids, DNA, and RNAs. EVs thus regulate gene expression and elicit biological effects including increased cell proliferation, migration, and adhesion in recipient cells. Uterine EVs are interactive and coordinate with ovarian progesterone (P4), trophectoderm-derived interferon tau (IFNT) and/or prostaglandins (PGs) in the physiological or pathological microenvironment. In this review, we will focus on intrauterine EVs in embryo–maternal interactions during the early stage of pregnancy, especially the implantation period in ruminant ungulates.

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“…For example, EVs delivering pathological prions could affect normal cells and EVs carrying miR-29b could impede the neuroprotective function in HIV patients (Hu et al, 2012; Berrone et al, 2015; Caruso Bavisotto et al, 2019). Cellular prion protein (PrPC) from plasma-derived EVs might contribute to the pathogenesis and propagation of prion diseases such as transmissible spongiform encephalopathies (TSEs), a type of neurodegenerative disease (Berrone et al, 2015; Properzi et al, 2015b). The study by Nonaka et al (2013) reported TAR DNA-binding protein 43 (TDP-43) aggregates to be potentially conveyed from one cell to the other, to some extent, via EVs.…”

Section: Prospective Role Of Evs In Sci Treatmentmentioning

confidence: 99%

Combinational Treatment of Bioscaffolds and Extracellular Vesicles in Spinal Cord Injury

Wang

Botchway

Zhang

et al. 2019

Front. Mol. Neurosci.

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Spinal cord injury (SCI) can result in an irreversible disability due to loss of sensorimotor function below the lesion. Presently, clinical treatments for SCI mainly include surgery, drugs and postoperative rehabilitation. The prospective roles of bioscaffolds and exosomes in several neurological diseases have been reported. Bioscaffolds can reconnect lesion gaps as well as transport cells and bioactive factors, which in turn can improve axonal and functional regeneration. Herein, we explicate the respective roles of bioscaffolds and exosomes in SCI, and elucidate on the usage of combinational therapy involving bioscaffolds and extracellular vesicles (EVs) in improving SCI.

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Detection of cellular prion protein in exosomes derived from ovine plasma

Cited by 17 publications

References 41 publications

Induction of IFNT-Stimulated Genes by Conceptus-Derived Exosomes during the Attachment Period

Induction of IFNT-Stimulated Genes by Conceptus-Derived Exosomes during the Attachment Period

Emerging Role of Extracellular Vesicles in Embryo–Maternal Communication throughout Implantation Processes

Combinational Treatment of Bioscaffolds and Extracellular Vesicles in Spinal Cord Injury

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